Cytological characterization of the bone marrow during fetal development in the alpaca (Vicugna pacos)

The present study aimed to investigate the morphology and morphometry of progenitor cells from the bone marrow through cytological examination during foetal development of the alpaca (Vicugna pacos). Bone marrow samples were collected from 18 culled and clinically healthy alpaca foetuses in Pasco, P...

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Authors: Calderón F., Elvis, Espinoza B., Juan, Li E., Olga, Barrios-Arpi, Manuel
Format: article
Status:Published version
Publication Date:2020
Country:Perú
Institution:Universidad Nacional Mayor de San Marcos
Repository:Revistas - Universidad Nacional Mayor de San Marcos
Language:Spanish
OAI Identifier:oai:revistasinvestigacion.unmsm.edu.pe:article/18156
Online Access:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/18156
Access Level:Open access
Keyword:alpaca
fetis
bone marrow
citology
feto
médula ósea
citología
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spelling Cytological characterization of the bone marrow during fetal development in the alpaca (Vicugna pacos)Caracterización citológica de la médula ósea durante el desarrollo fetal en la alpaca (Vicugna pacos)Calderón F., ElvisEspinoza B., JuanLi E., OlgaBarrios-Arpi, Manuelalpacafetisbone marrowcitologyalpacafetomédula óseacitologíaThe present study aimed to investigate the morphology and morphometry of progenitor cells from the bone marrow through cytological examination during foetal development of the alpaca (Vicugna pacos). Bone marrow samples were collected from 18 culled and clinically healthy alpaca foetuses in Pasco, Peru. The eighth and ninth ribs were removed using the cost-chondral junction as reference and smears were made with the bone marrow samples, and then stained with Wright. The percentages of cellularity for each third of pregnancy were 16 ± 0.2 (n= 3), 35 ± 0.16 (n= 5) and 78 ± 0.12 (n=10), respectively, showing the first hematopoietic cells from 201.5 days old. Through the differential count of 500 cells, the myeloid: erythroid ratio was established, being 0.47 ± 0.27 and 0.69 ± 0.19 for the first, second and last third of pregnancy, respectively. In the second third of pregnancy 10.17% of erythroid cells, 5.03% of myeloid cells and 84.80% of other cell types were detected, while in the last third it was 39.99% of erythroid cells, 26.99% of myeloid cells and 33.21% of other cell types. In the morphometric examination, hematopoietic cells revealed characteristics like those found in other species.El presente estudio tuvo como objetivo investigar la morfología y morfometría de las células progenitoras de la médula ósea a través del examen citológico durante el desarrollo fetal de la alpaca (Vicugna pacos). Se colectaron muestras de médula ósea provenientes de 18 fetos de alpacas clínicamente sanas y destinadas a beneficio en Pasco, Perú. Se seccionaron la octava y novena costillas tomando como referencia la unión costo-condral y se hicieron frotis con las muestras de médula ósea, que fueron coloreadas con la tinción Wright. Los porcentajes de celularidad para cada tercio de gestación fueron 16 ± 0.2 (n=3), 35 ± 0.16 (n=5) y 78 ± 0.12 (n=10), respectivamente, evidenciándose las primeras células hematopoyéticas a partir de los 201.5 días de edad. Mediante el conteo diferencial de 500 células se estableció la relación mieloide:eritroide, siendo de 0, 0.47 ± 0.27 y 0.69 ± 0.19 para el primer, segundo y tercer tercio de gestación, respectivamente. En el segundo tercio de gestación se detectó 10.17% de células eritroides, 5.03% de células mieloides y 84.80% de otros tipos celulares, mientras que en el último tercio fue de 39.99% de células eritroides, 26.99% de células mieloides y 33.21% de otros tipos celulares. En el examen morfométrico, las células hematopoyéticas revelaron características similares a las encontradas en otras especies.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2020-08-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1815610.15381/rivep.v31i3.18156Revista de Investigaciones Veterinarias del Perú; Vol. 31 No. 3 (2020); e18156Revista de Investigaciones Veterinarias del Perú; Vol. 31 Núm. 3 (2020); e181561682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/18156/15478Derechos de autor 2020 Elvis Calderón F., Juan Espinoza B., Olga Li E., Manuel Barrios-Arpihttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:revistasinvestigacion.unmsm.edu.pe:article/181562020-08-11T16:20:53Z
dc.title.none.fl_str_mv Cytological characterization of the bone marrow during fetal development in the alpaca (Vicugna pacos)
Caracterización citológica de la médula ósea durante el desarrollo fetal en la alpaca (Vicugna pacos)
title Cytological characterization of the bone marrow during fetal development in the alpaca (Vicugna pacos)
spellingShingle Cytological characterization of the bone marrow during fetal development in the alpaca (Vicugna pacos)
Calderón F., Elvis
alpaca
fetis
bone marrow
citology
alpaca
feto
médula ósea
citología
title_short Cytological characterization of the bone marrow during fetal development in the alpaca (Vicugna pacos)
title_full Cytological characterization of the bone marrow during fetal development in the alpaca (Vicugna pacos)
title_fullStr Cytological characterization of the bone marrow during fetal development in the alpaca (Vicugna pacos)
title_full_unstemmed Cytological characterization of the bone marrow during fetal development in the alpaca (Vicugna pacos)
title_sort Cytological characterization of the bone marrow during fetal development in the alpaca (Vicugna pacos)
dc.creator.none.fl_str_mv Calderón F., Elvis
Espinoza B., Juan
Li E., Olga
Barrios-Arpi, Manuel
author Calderón F., Elvis
author_facet Calderón F., Elvis
Espinoza B., Juan
Li E., Olga
Barrios-Arpi, Manuel
author_role author
author2 Espinoza B., Juan
Li E., Olga
Barrios-Arpi, Manuel
author2_role author
author
author
dc.subject.none.fl_str_mv alpaca
fetis
bone marrow
citology
alpaca
feto
médula ósea
citología
topic alpaca
fetis
bone marrow
citology
alpaca
feto
médula ósea
citología
description The present study aimed to investigate the morphology and morphometry of progenitor cells from the bone marrow through cytological examination during foetal development of the alpaca (Vicugna pacos). Bone marrow samples were collected from 18 culled and clinically healthy alpaca foetuses in Pasco, Peru. The eighth and ninth ribs were removed using the cost-chondral junction as reference and smears were made with the bone marrow samples, and then stained with Wright. The percentages of cellularity for each third of pregnancy were 16 ± 0.2 (n= 3), 35 ± 0.16 (n= 5) and 78 ± 0.12 (n=10), respectively, showing the first hematopoietic cells from 201.5 days old. Through the differential count of 500 cells, the myeloid: erythroid ratio was established, being 0.47 ± 0.27 and 0.69 ± 0.19 for the first, second and last third of pregnancy, respectively. In the second third of pregnancy 10.17% of erythroid cells, 5.03% of myeloid cells and 84.80% of other cell types were detected, while in the last third it was 39.99% of erythroid cells, 26.99% of myeloid cells and 33.21% of other cell types. In the morphometric examination, hematopoietic cells revealed characteristics like those found in other species.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/18156
10.15381/rivep.v31i3.18156
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/18156
identifier_str_mv 10.15381/rivep.v31i3.18156
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/18156/15478
dc.rights.none.fl_str_mv Derechos de autor 2020 Elvis Calderón F., Juan Espinoza B., Olga Li E., Manuel Barrios-Arpi
https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2020 Elvis Calderón F., Juan Espinoza B., Olga Li E., Manuel Barrios-Arpi
https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
dc.source.none.fl_str_mv Revista de Investigaciones Veterinarias del Perú; Vol. 31 No. 3 (2020); e18156
Revista de Investigaciones Veterinarias del Perú; Vol. 31 Núm. 3 (2020); e18156
1682-3419
1609-9117
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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