Análisis electroforético de proteínas del veneno de Bothrops atrox “jergón” (Ofidia: Viperidae) de distintas zonas geográficas de la Amazonia Peruana
The snake´s venoms are endogenous secretions constituted for proteins and can produce different symptomatology and in some cases the dead. Using the electrophoretic technique in polyacrylamide discontinuous gel of 7.5% and 12% whit sodium dodecylsulphate (PAGE-SDS) in reductions conditions were carr...
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2019 |
| País: | Perú |
| Recursos: | Centro de Preparación para la Ciencia y Tecnología |
| Repositorio: | ECIPERÚ |
| Idioma: | español |
| OAI Identifier: | oai:revistas.eciperu.net:article/243 |
| Acesso em linha: | https://revistas.eciperu.net/index.php/ECIPERU/article/view/243 |
| Access Level: | acceso abierto |
| Palavra-chave: | venenos, proteínas, electroforesis, poliacrilamida, cuantificación. venoms, proteins, electrophoretic, polyacrylamide, quantification. |
| Resumo: | The snake´s venoms are endogenous secretions constituted for proteins and can produce different symptomatology and in some cases the dead. Using the electrophoretic technique in polyacrylamide discontinuous gel of 7.5% and 12% whit sodium dodecylsulphate (PAGE-SDS) in reductions conditions were carried on a protein analysis of the venom in 5 Bothrops atrox snake´s from 5 different geographical zones to the Peruvian Amazon using centrifuge crude venom, additionally the proteins were quantification for using an spectrophotometer following the Biuret method. The results evidence the presents of different bands in the proteins, in ranges of 14 kD to 116 kD, finding 9, 11,8,7,8 bands of proteins for the populations of Moena Caño, Isla Iquitos, Panguana, Genaro Herrera and Nina Rumi, while the quantification show the presents to major quantity of protein in the snake from Isla Iquitos (67.41 mg/ml) and a less quantity in the specie from Genaro Herrera (32.13 mg/ml). We conclude mentioning the existence of variations in the electrophoretic profile and quantification to proteins from the venom of Bothrops atrox to the Peruvian Amazon. |
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