NMR-based leaf metabolic profiling of V. planifolia and three endemic Vanilla species from the Peruvian Amazon

The fruit of Vanilla planifolia is broadly preferred by the agroindustry and gourmet markets due to its refined flavor and aroma. Peruvian Vanilla has been proposed as a possible source for genetic improvement of existing Vanilla cultivars, but, little has been done to facilitate comprehensive studi...

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Detalles Bibliográficos
Autores: Leyva V.E., Lopez J.M., Zevallos-Ventura A., Cabrera R., Cañari-Chumpitaz C., Toubiana D., Maruenda H.
Tipo de recurso: artículo
Fecha de publicación:2021
País:Perú
Institución:Consejo Nacional de Ciencia Tecnología e Innovación
Repositorio:CONCYTEC-Institucional
Idioma:inglés
OAI Identifier:oai:repositorio.concytec.gob.pe:20.500.12390/2314
Acceso en línea:https://hdl.handle.net/20.500.12390/2314
https://doi.org/10.1016/j.foodchem.2021.129365
Access Level:acceso abierto
Palabra clave:V. ribeiroi
4-Hydroxybenzyl alcohol glucoside
Glucosides A and B
NMR metabolomics
Quantitative NMR
V. palmarum
V. planifolia
V. pompona
http://purl.org/pe-repo/ocde/ford#3.02.18
Descripción
Sumario:The fruit of Vanilla planifolia is broadly preferred by the agroindustry and gourmet markets due to its refined flavor and aroma. Peruvian Vanilla has been proposed as a possible source for genetic improvement of existing Vanilla cultivars, but, little has been done to facilitate comprehensive studies of these and other Vanilla. Here, a nuclear magnetic resonance (NMR) metabolomic platform was developed to profile for the first time the leaves – organ known to accumulate vanillin putative precursors – of V. planifolia and those of Peruvian V. pompona, V. palmarum, and V. ribeiroi, with the aim to determine metabolic differences among them. Analysis of the NMR spectra allowed the identification of thirty-six metabolites, twenty-five of which were quantified. One-way ANOVA and post-hoc Tukey test revealed that these metabolites changed significantly among species, whilst multivariate-analyses allowed the identification of malic and homocitric acids, together with two vanillin precursors, as relevant metabolic markers for species differentiation. © 2021 Elsevier Ltd