Detection of Fasciola hepatica by indirect ELISA in sheep and goats from Boavita, Colombia

The aim of this study was to determine the seroprevalence of Fasciola hepatica by indirect ELISA in sheep and goats from Boavita (Boyaca, Colombia). A descriptive cross-sectional study with simple random sampling was carried out. A total of 297 blood samples from sheep and 337 from goats of differen...

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Bibliographic Details
Authors: Cruz- Estupiñan, Sharon E., Bulla-Castañeda, Diana M., Lancheros-Buitrago, Deisy J., Garcia-Corredor, Diego J., Giraldo Forero, Julio C., Pulido-Medellín, Martín O.
Format: article
Status:Published version
Publication Date:2023
Country:Perú
Institution:Universidad Nacional Mayor de San Marcos
Repository:Revistas - Universidad Nacional Mayor de San Marcos
Language:Spanish
OAI Identifier:oai:revistasinvestigacion.unmsm.edu.pe:article/26378
Online Access:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/26378
Access Level:Open access
Keyword:Fasciola hepatica
sheep
goats
indirect ELISA
seroprevalence
ovejas
cabras
ELISA indirecto
seroprevalencia
Description
Summary:The aim of this study was to determine the seroprevalence of Fasciola hepatica by indirect ELISA in sheep and goats from Boavita (Boyaca, Colombia). A descriptive cross-sectional study with simple random sampling was carried out. A total of 297 blood samples from sheep and 337 from goats of different breeds and age groups were collected. The sera were analyzed by the indirect ELISA technique with the commercial ELISA kit BIO K 211 - Monoscreen AbELISA F. hepatica. Additionally, an epidemiological survey was carried out. The seroprevalence of F. hepatica was 67.34% (200/297) in sheep and 59.94% (202/337) in goats. Likewise, seroprevalence was higher in males (sheep: 77.78%, 21/27; goats: 63.89%, 23/36), in sheep older than 3 years (83.33%, 50/60) and in goats less than 1 year of age. (69.77%, 60/86), as well as in Creole breed (sheep: 69.84%; goats: 61.79%). Sheep over 3 years of age were determined as a risk factor, while in goats the Creole breed variables and extensive grazing were identified as risk factors.

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