Development of a 16S rRNA PCR-RFLP Assay for Bartonella Identification: Applicability in the Identification of Species Involved in Human Infections

Abstract We designed a 16S rRNA gene PCR-RFLP scheme to identify all currently described Bartonella spp. The 16S rRNA genes of all Bartonella spp. were in-silico analyzed in order to design a RFLP technique able to discriminate among different species. The restriction enzymes selected were MaeIII, M...

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Bibliographic Details
Authors: Del Valle, Luis J., Jaramillo, Michael L., Talledo, Miguel, Pons, Maria J., Flores, Lidia, Quispe, Ruth L., Ramírez, Pablo, García de la Guarda, Ruth, Alvarado, Débora, Espinoza-Culupú, Abraham, Del Valle Mendoza, Juana, Vargas, Martha, Ruíz, Joaquim
Format: article
Publication Date:2014
Country:Perú
Institution:Universidad Peruana de Ciencias Aplicadas
Repository:UPC-Institucional
Language:English
OAI Identifier:oai:repositorioacademico.upc.edu.pe:10757/322342
Online Access:https://doi.org/10.13189/ujmr.2014.020102
http://hdl.handle.net/10757/322342
Access Level:Open access
Keyword:Bartonella
PCR-RFLP
16s rRNA Gene
Identification
Description
Summary:Abstract We designed a 16S rRNA gene PCR-RFLP scheme to identify all currently described Bartonella spp. The 16S rRNA genes of all Bartonella spp. were in-silico analyzed in order to design a RFLP technique able to discriminate among different species. The restriction enzymes selected were MaeIII, MseI, Sau96I, BsaAI, DrdI, FokI, BssHII, BstUI, AluI, TspDTI and HphI which, according to a decision-making tree, facilitated the differentiation of all the currently described species of Bartonella.The technique was experimentally tested in different species of Bartonella, including human pathogenic B. bacilliformis and B. henselae with a 100% of concordance with the in-silico predicted patterns.This novel RFLP assay could be used to identify both human and non-human pathogenic Bartonella in diagnostic, phylogenetic and epidemiologic studies.

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