Interleukin 10 (IL-10) and transforming growth factor beta (TGF- ß) expression in intestinal mucosa of alpaca crias (Vicugna pacos)
The aim of the present study was to evaluate the relative expression levels of cytokines IL-10 and TGF-β in the intestinal mucosa of clinically healthy alpacas from 2 to 47 days old. The animals were classified in three age groups (six animals per group): 2-8 days old (group 1), 10-21days old (group...
| Autores: | , , , , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2017 |
| País: | Perú |
| Recursos: | Universidad Nacional Mayor de San Marcos |
| Repositorio: | Revistas - Universidad Nacional Mayor de San Marcos |
| Idioma: | español |
| OAI Identifier: | oai:revistasinvestigacion.unmsm.edu.pe:article/13082 |
| Acesso em linha: | https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/13082 |
| Access Level: | acceso abierto |
| Palavra-chave: | IL-10 TFG-ß T regulador alpaca intestino RT-PCR tiempo real TFG-B T regulator intestine real time RT-PCR |
| Resumo: | The aim of the present study was to evaluate the relative expression levels of cytokines IL-10 and TGF-β in the intestinal mucosa of clinically healthy alpacas from 2 to 47 days old. The animals were classified in three age groups (six animals per group): 2-8 days old (group 1), 10-21days old (group 2) and 26-47-days old (group 3), regardless sex or breed. The midportion of jejunum of each animal was collected; total RNA was extracted and then analyzed by real-time RT-PCR technique using IL-10 and TGF-β specific primers. The relative mRNA expression analysis was done using the comparative 2-ΔΔCt method. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as housekeeping gene and jejunum of three 11-month-old fetuses were used as calibrators. The results showed that IL-10 mRNA expression levels were 7.21±1.02 (group 1), 13.53±1.26 (group 2), and 18.77±1.48 (group 3) times as expressed by the calibrate group with significant difference between age groups (p<0.05). Also, TGF-β mRNA expression levels were 2.18±0.23 (group 1), 3.03±0.18 (group 2), and 4.06±0.15 (group 3) times as expressed by the calibrate group with significant difference between age groups (p<0.05). The expression of IL-10 and TGF-β mRNA was age dependent, increasing significantly in older animals. |
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