Características histológicas de la dentina bovina mediante tinción Tricrómica de Masson

Objectives: Identify the histological characteristics of bovine dentin using histological technique by decalcification with Masson's Trichrome staining. Materials and Methods: This was an observational and descriptive study using units of analysis. Bovine teeth were used, which were subjected t...

Descripción completa

Detalles Bibliográficos
Autores: Lezcano, Melisa Raquel, Enz, Nathalie, Affur, María Constanza, Gili, María Alejandra
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:Perú
Institución:Universidad Científica del Sur
Repositorio:Revistas - Universidad Científica del Sur
Idioma:español
OAI Identifier:oai:revistas.cientifica.edu.pe:article/1732
Acceso en línea:https://revistas.cientifica.edu.pe/index.php/odontologica/article/view/1732
Access Level:acceso abierto
Palabra clave:dentin
bovine tooth
decalcification
Masson's trichrome
dentina
diente bovino
descalcificación
tricrómica de Masson
Descripción
Sumario:Objectives: Identify the histological characteristics of bovine dentin using histological technique by decalcification with Masson's Trichrome staining. Materials and Methods: This was an observational and descriptive study using units of analysis. Bovine teeth were used, which were subjected to a decalcification technique and Masson's trichrome staining. Microscopic observation and registration of the structures was carried out. Results: In the microscopic observation of the teeth subjected to decalcification, dentin, predentin and the structural organization of the tubular and peritubular dentin were observed. The odontoblasts arranged in a palisade with the odontoblastic process included within the dentinal tubule and the periprocedural space. The presence of interglobular dentin is not observed in areas of coronary dentin. The histomorphological pattern corresponding to dentin-predentin-pulp is arranged in a similar way to human teeth. Conclusions: Bovine dentin constitutes an ideal substrate for in vitro research work with dental biomaterials, since it presents histological characteristics similar to bovine teeth; the absence of interglobular dentin would not represent a significant difference according to what was observed in this study.