MOLECULAR CHARACTERIZATION OF CRUDE SEED EXTRACTS FROM Moringa oleifera

Some natural coagulants have shown to be efficient in removing turbidity and color from water, including the extract of Moringa oleifera seeds. In this study, the nature of the coagulant in M. oleifera was determined by establishing the protein content in extracted solutions and the corresponding mo...

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Autores: María Martha Sandoval Arreola, José Ramón Laines Canepa, José Roberto Hernández Barajas
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:México
Institución:Universidad Juárez Autónoma de Tabasco
Repositorio:Redalyc-UJAT
OAI Identifier:oai:redalyc.org:33946993006
Acceso en línea:https://www.redalyc.org/articulo.oa?id=33946993006
Access Level:acceso abierto
Palabra clave:Multidisciplinarias (Ciencias Sociales)
Seed Extract
Electrophoresis
Moringa oleifera
Coagulating Protein
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spelling MOLECULAR CHARACTERIZATION OF CRUDE SEED EXTRACTS FROM Moringa oleiferaMaría Martha Sandoval ArreolaJosé Ramón Laines CanepaJosé Roberto Hernández BarajasMultidisciplinarias (Ciencias Sociales)Seed ExtractElectrophoresisMoringa oleiferaCoagulating ProteinSome natural coagulants have shown to be efficient in removing turbidity and color from water, including the extract of Moringa oleifera seeds. In this study, the nature of the coagulant in M. oleifera was determined by establishing the protein content in extracted solutions and the corresponding molecular weight. The extraction of coagulant from M. oleifera was performed using three types of solvents: distilled water, seawater, and a NaCl 1N solution. The protein content of the extracts was determined by the Pierce BCA colorimetric assay, using a Genesis 10 S UV- VIS Spectrofotometer ® . For the determination of protein molecu - lar weight, the electrophoresis in polyacrylamide gel (SDS) tech - nique in 12% gel was used, following Laemmli protocol. Each coagulant was prepared using the Okuda method, modifying the extraction process using liquid nitrogen. Protein content was 3160, 3400, and 3640mg BSA·l -1 , for distilled water, seawater, and NaCl solution, respectively. The molecular weight deter - mined for the Moringa coagulant extracted with distilled water corresponds to a dimer with a weight of 16 and 14kDa for each band. The molecular weight for the salt solutions was the same, 32.35, 6.00 and 5.00kDa for each band. The results show that the modified method for the extraction of the coagulant liquid N 2 yielded homogeneous protein content in each of the solutions and a higher fragmentation with salt solutions. This fragmenta - tion allowed saline solutions to achieve greater removal of tur - bidity and color. Therefore, Moringa seeds is a promising alter - native for water treatment.Asociación Interciencia2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdf0378-1844https://www.redalyc.org/articulo.oa?id=33946993006Interciencia (República Bolivariana de Venezuela) Num.8 Vol.41reponame:Redalyc-UJATinstname:Universidad Juárez Autónoma de Tabascoinstacron:UJATenhttp://www.redalyc.org/revista.oa?id=339Intercienciainfo:eu-repo/semantics/openAccessoai:redalyc.org:339469930062024-08-23T16:10:14Z
dc.title.none.fl_str_mv MOLECULAR CHARACTERIZATION OF CRUDE SEED EXTRACTS FROM Moringa oleifera
title MOLECULAR CHARACTERIZATION OF CRUDE SEED EXTRACTS FROM Moringa oleifera
spellingShingle MOLECULAR CHARACTERIZATION OF CRUDE SEED EXTRACTS FROM Moringa oleifera
María Martha Sandoval Arreola
Multidisciplinarias (Ciencias Sociales)
Seed Extract
Electrophoresis
Moringa oleifera
Coagulating Protein
title_short MOLECULAR CHARACTERIZATION OF CRUDE SEED EXTRACTS FROM Moringa oleifera
title_full MOLECULAR CHARACTERIZATION OF CRUDE SEED EXTRACTS FROM Moringa oleifera
title_fullStr MOLECULAR CHARACTERIZATION OF CRUDE SEED EXTRACTS FROM Moringa oleifera
title_full_unstemmed MOLECULAR CHARACTERIZATION OF CRUDE SEED EXTRACTS FROM Moringa oleifera
title_sort MOLECULAR CHARACTERIZATION OF CRUDE SEED EXTRACTS FROM Moringa oleifera
dc.creator.none.fl_str_mv María Martha Sandoval Arreola
José Ramón Laines Canepa
José Roberto Hernández Barajas
author María Martha Sandoval Arreola
author_facet María Martha Sandoval Arreola
José Ramón Laines Canepa
José Roberto Hernández Barajas
author_role author
author2 José Ramón Laines Canepa
José Roberto Hernández Barajas
author2_role author
author
dc.subject.none.fl_str_mv Multidisciplinarias (Ciencias Sociales)
Seed Extract
Electrophoresis
Moringa oleifera
Coagulating Protein
topic Multidisciplinarias (Ciencias Sociales)
Seed Extract
Electrophoresis
Moringa oleifera
Coagulating Protein
description Some natural coagulants have shown to be efficient in removing turbidity and color from water, including the extract of Moringa oleifera seeds. In this study, the nature of the coagulant in M. oleifera was determined by establishing the protein content in extracted solutions and the corresponding molecular weight. The extraction of coagulant from M. oleifera was performed using three types of solvents: distilled water, seawater, and a NaCl 1N solution. The protein content of the extracts was determined by the Pierce BCA colorimetric assay, using a Genesis 10 S UV- VIS Spectrofotometer ® . For the determination of protein molecu - lar weight, the electrophoresis in polyacrylamide gel (SDS) tech - nique in 12% gel was used, following Laemmli protocol. Each coagulant was prepared using the Okuda method, modifying the extraction process using liquid nitrogen. Protein content was 3160, 3400, and 3640mg BSA·l -1 , for distilled water, seawater, and NaCl solution, respectively. The molecular weight deter - mined for the Moringa coagulant extracted with distilled water corresponds to a dimer with a weight of 16 and 14kDa for each band. The molecular weight for the salt solutions was the same, 32.35, 6.00 and 5.00kDa for each band. The results show that the modified method for the extraction of the coagulant liquid N 2 yielded homogeneous protein content in each of the solutions and a higher fragmentation with salt solutions. This fragmenta - tion allowed saline solutions to achieve greater removal of tur - bidity and color. Therefore, Moringa seeds is a promising alter - native for water treatment.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 0378-1844
https://www.redalyc.org/articulo.oa?id=33946993006
identifier_str_mv 0378-1844
url https://www.redalyc.org/articulo.oa?id=33946993006
dc.language.none.fl_str_mv en
language_invalid_str_mv en
dc.relation.none.fl_str_mv http://www.redalyc.org/revista.oa?id=339
dc.rights.none.fl_str_mv Interciencia
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Interciencia
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Asociación Interciencia
publisher.none.fl_str_mv Asociación Interciencia
dc.source.none.fl_str_mv Interciencia (República Bolivariana de Venezuela) Num.8 Vol.41
reponame:Redalyc-UJAT
instname:Universidad Juárez Autónoma de Tabasco
instacron:UJAT
instname_str Universidad Juárez Autónoma de Tabasco
instacron_str UJAT
institution UJAT
reponame_str Redalyc-UJAT
collection Redalyc-UJAT
repository.name.fl_str_mv
repository.mail.fl_str_mv
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