Antimicrobial Activity of Diverse Chemotypes of Lippia graveolens Against Aeromonas hydrophila Isolated from Oreochromis niloticus

(Objective) This study aimed to evaluate the antimicrobial efficacy of essential oil (EO) from diverse chemotypes of Lippia graveolens against oxytetracycline-resistant Aeromonas hydrophila, which primarily affects the tilapia aquaculture (O. niloticus) in Guatemala.(Methodology) L. graveolens were...

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Autores: Josué García-Pérez, Juan Pérez-Sabino, Susana Mendoza-Elvira, Antonio Jorge Ribeiro da Silva, Juan Ulloa-Rojas
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:México
Institución:Universidad Nacional Autónoma de México
Repositorio:Redalyc-UNAM
OAI Identifier:oai:redalyc.org:475981034004
Acceso en línea:https://www.redalyc.org/articulo.oa?id=475981034004
https://www.redalyc.org/journal/4759/475981034004/
https://www.redalyc.org/journal/4759/475981034004/html/
https://www.redalyc.org/journal/4759/475981034004/475981034004.epub
https://www.redalyc.org/journal/4759/475981034004/movil
Access Level:acceso abierto
Palabra clave:Multidisciplinaria (Ciencias Naturales y Exactas)
thymol
oregano
tilapia
aquaculture
Essential oil
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spelling Antimicrobial Activity of Diverse Chemotypes of Lippia graveolens Against Aeromonas hydrophila Isolated from Oreochromis niloticusJosué García-PérezJuan Pérez-SabinoSusana Mendoza-ElviraAntonio Jorge Ribeiro da SilvaJuan Ulloa-RojasMultidisciplinaria (Ciencias Naturales y Exactas)thymoloreganotilapiaaquacultureEssential oil(Objective) This study aimed to evaluate the antimicrobial efficacy of essential oil (EO) from diverse chemotypes of Lippia graveolens against oxytetracycline-resistant Aeromonas hydrophila, which primarily affects the tilapia aquaculture (O. niloticus) in Guatemala.(Methodology) L. graveolens were collected in three departments in Guatemala. The EO was obtained by hydrodistillation and characterized using gas chromatography and mass spectrometry (GC/MS). Subsequently, an antimicrobial assay was conducted using disk and dilution susceptibility tests and evaluation of synergistic interactions among the chemotypes. Each test was performed in triplicate.(Results) The analysis revealed the presence of twenty-seven compounds in the EO obtained from the chemotypes, with the main class being monoterpenes. The major constituents identified were cis-Dihydro-β-terpineol (8.84%) in chemotype I, carvacrol (51.82%) in chemotype II, and thymol (79.62%) in chemotype III. All EO chemotypes of L. graveolens demonstrated the ability to inhibit the A. hydrophila growth. Thymol chemotype exhibited the strongest inhibitory effect against bacterial growth, with a minimum inhibitory concentration (MIC) of 92.4 µg/mL and a minimum bactericidal concentration (MBC) of 184.8 µg/mL. Furthermore, the results suggest that there is no synergistic or additive effect when combining different chemotypes of L. graveolens.(Conclusions) This is the first report of L. graveolens chemotypes exhibiting antimicrobial activity against oxytetracycline-resistant A. hydrophila. The findings suggest that the chemotype thymol could be a potential treatment for infections in the tilapia aquaculture in Guatemala.Universidad Nacional2024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdf2215-3470https://www.redalyc.org/articulo.oa?id=475981034004https://www.redalyc.org/journal/4759/475981034004/https://www.redalyc.org/journal/4759/475981034004/html/https://www.redalyc.org/journal/4759/475981034004/475981034004.epubhttps://www.redalyc.org/journal/4759/475981034004/movil10.15359/ru.38-1.30Uniciencia (Costa Rica) Num.1 Vol.38reponame:Redalyc-UNAMinstname:Universidad Nacional Autónoma de Méxicoinstacron:UNAMenhttp://www.redalyc.org/revista.oa?id=4759Unicienciainfo:eu-repo/semantics/openAccessoai:redalyc.org:4759810340042025-09-03T18:11:24Z
dc.title.none.fl_str_mv Antimicrobial Activity of Diverse Chemotypes of Lippia graveolens Against Aeromonas hydrophila Isolated from Oreochromis niloticus
title Antimicrobial Activity of Diverse Chemotypes of Lippia graveolens Against Aeromonas hydrophila Isolated from Oreochromis niloticus
spellingShingle Antimicrobial Activity of Diverse Chemotypes of Lippia graveolens Against Aeromonas hydrophila Isolated from Oreochromis niloticus
Josué García-Pérez
Multidisciplinaria (Ciencias Naturales y Exactas)
thymol
oregano
tilapia
aquaculture
Essential oil
title_short Antimicrobial Activity of Diverse Chemotypes of Lippia graveolens Against Aeromonas hydrophila Isolated from Oreochromis niloticus
title_full Antimicrobial Activity of Diverse Chemotypes of Lippia graveolens Against Aeromonas hydrophila Isolated from Oreochromis niloticus
title_fullStr Antimicrobial Activity of Diverse Chemotypes of Lippia graveolens Against Aeromonas hydrophila Isolated from Oreochromis niloticus
title_full_unstemmed Antimicrobial Activity of Diverse Chemotypes of Lippia graveolens Against Aeromonas hydrophila Isolated from Oreochromis niloticus
title_sort Antimicrobial Activity of Diverse Chemotypes of Lippia graveolens Against Aeromonas hydrophila Isolated from Oreochromis niloticus
dc.creator.none.fl_str_mv Josué García-Pérez
Juan Pérez-Sabino
Susana Mendoza-Elvira
Antonio Jorge Ribeiro da Silva
Juan Ulloa-Rojas
author Josué García-Pérez
author_facet Josué García-Pérez
Juan Pérez-Sabino
Susana Mendoza-Elvira
Antonio Jorge Ribeiro da Silva
Juan Ulloa-Rojas
author_role author
author2 Juan Pérez-Sabino
Susana Mendoza-Elvira
Antonio Jorge Ribeiro da Silva
Juan Ulloa-Rojas
author2_role author
author
author
author
dc.subject.none.fl_str_mv Multidisciplinaria (Ciencias Naturales y Exactas)
thymol
oregano
tilapia
aquaculture
Essential oil
topic Multidisciplinaria (Ciencias Naturales y Exactas)
thymol
oregano
tilapia
aquaculture
Essential oil
description (Objective) This study aimed to evaluate the antimicrobial efficacy of essential oil (EO) from diverse chemotypes of Lippia graveolens against oxytetracycline-resistant Aeromonas hydrophila, which primarily affects the tilapia aquaculture (O. niloticus) in Guatemala.(Methodology) L. graveolens were collected in three departments in Guatemala. The EO was obtained by hydrodistillation and characterized using gas chromatography and mass spectrometry (GC/MS). Subsequently, an antimicrobial assay was conducted using disk and dilution susceptibility tests and evaluation of synergistic interactions among the chemotypes. Each test was performed in triplicate.(Results) The analysis revealed the presence of twenty-seven compounds in the EO obtained from the chemotypes, with the main class being monoterpenes. The major constituents identified were cis-Dihydro-β-terpineol (8.84%) in chemotype I, carvacrol (51.82%) in chemotype II, and thymol (79.62%) in chemotype III. All EO chemotypes of L. graveolens demonstrated the ability to inhibit the A. hydrophila growth. Thymol chemotype exhibited the strongest inhibitory effect against bacterial growth, with a minimum inhibitory concentration (MIC) of 92.4 µg/mL and a minimum bactericidal concentration (MBC) of 184.8 µg/mL. Furthermore, the results suggest that there is no synergistic or additive effect when combining different chemotypes of L. graveolens.(Conclusions) This is the first report of L. graveolens chemotypes exhibiting antimicrobial activity against oxytetracycline-resistant A. hydrophila. The findings suggest that the chemotype thymol could be a potential treatment for infections in the tilapia aquaculture in Guatemala.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 2215-3470
https://www.redalyc.org/articulo.oa?id=475981034004
https://www.redalyc.org/journal/4759/475981034004/
https://www.redalyc.org/journal/4759/475981034004/html/
https://www.redalyc.org/journal/4759/475981034004/475981034004.epub
https://www.redalyc.org/journal/4759/475981034004/movil
10.15359/ru.38-1.30
identifier_str_mv 2215-3470
10.15359/ru.38-1.30
url https://www.redalyc.org/articulo.oa?id=475981034004
https://www.redalyc.org/journal/4759/475981034004/
https://www.redalyc.org/journal/4759/475981034004/html/
https://www.redalyc.org/journal/4759/475981034004/475981034004.epub
https://www.redalyc.org/journal/4759/475981034004/movil
dc.language.none.fl_str_mv en
language_invalid_str_mv en
dc.relation.none.fl_str_mv http://www.redalyc.org/revista.oa?id=4759
dc.rights.none.fl_str_mv Uniciencia
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Uniciencia
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional
publisher.none.fl_str_mv Universidad Nacional
dc.source.none.fl_str_mv Uniciencia (Costa Rica) Num.1 Vol.38
reponame:Redalyc-UNAM
instname:Universidad Nacional Autónoma de México
instacron:UNAM
instname_str Universidad Nacional Autónoma de México
instacron_str UNAM
institution UNAM
reponame_str Redalyc-UNAM
collection Redalyc-UNAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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