Antibacterial effect of biosynthesized silver nanoparticles in Pacific white shrimp Litopenaeus vannamei (Boone) infected with necrotizing hepatopancreatitis bacterium (NHP - B)

The shrimp necrotizing hepatopancreatitis is caused by a pleomorphic Gram - negative Rickettsia - like bacteria known as NHP - B. It is well known that silver is an effective bactericidal, but experiments with aquatic organisms are scarce, even more at nanoparticle level. The objective of this study...

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Detalles Bibliográficos
Autores: Martín Rodrigo Acedo-Valdez, José Manuel Grijalva-Chon, Eduardo Larios-Rodríguez, Amir Darío Maldonado-Arce, Fernando Mendoza-Cano, José Arturo Sánchez-Paz, Reina Castro-Longoria
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:México
Institución:Universidad de Sonora
Repositorio:Redalyc-USON
OAI Identifier:oai:redalyc.org:175051041017
Acceso en línea:https://www.redalyc.org/articulo.oa?id=175051041017
Access Level:acceso abierto
Palabra clave:Ciencias de la Tierra
Camellia sinensis
Azadirachta indica
Pacific white shrimp
Descripción
Sumario:The shrimp necrotizing hepatopancreatitis is caused by a pleomorphic Gram - negative Rickettsia - like bacteria known as NHP - B. It is well known that silver is an effective bactericidal, but experiments with aquatic organisms are scarce, even more at nanoparticle level. The objective of this study was to determine the antibacterial effect of silver nanoparticles (AgNP) against NHP - B in the Pacific white shrimp Litopenaeus vannamei . For this, AgNO 3 was used as source of silver, and extracts of dried leaves of green tea Camellia sinensis and neem tree Azadirachta indica as reducing agents. Different lots of infected shrimp received 0,5 and 35 μg of AgNP by forced feeding. The differences between the number of bacterial nodules in shrimp hepatopancreas and shrimp mortality relative to the amount of AgNP demonstrated to be effective against this pathogen.