Toxicity induced by dieldrin and chlorpyrifos in the freshwater crayfish Cambarellus montezumae (Cambaridae)

The toxicity induced by insecticides in aquatic organisms is of utmost relevance because it may give a clue about the degree of health or damage of the involved ecosystem. In the present report, we determined the effect of dieldrin (DD) and chlorpyrifos (CP) on the freshwater crayfish, Cambarellus m...

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Autores: Sandra Díaz-Barriga Arceo, Laura Martínez-Tabche, Isela Álvarez-González, Eugenia López López, Eduardo Madrigal-Bujaidar
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:México
Institución:Universidad Nacional Autónoma de México
Repositorio:Redalyc-UNAM
OAI Identifier:oai:redalyc.org:44933764008
Acceso en línea:https://www.redalyc.org/articulo.oa?id=44933764008
Access Level:acceso abierto
Palabra clave:Biología
dieldrin
crayfish
Genotoxicity
chlorpyrifos
lipoperoxidation
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spelling Toxicity induced by dieldrin and chlorpyrifos in the freshwater crayfish Cambarellus montezumae (Cambaridae)Sandra Díaz-Barriga ArceoLaura Martínez-TabcheIsela Álvarez-GonzálezEugenia López LópezEduardo Madrigal-BujaidarBiologíadieldrincrayfishGenotoxicitychlorpyrifoslipoperoxidationThe toxicity induced by insecticides in aquatic organisms is of utmost relevance because it may give a clue about the degree of health or damage of the involved ecosystem. In the present report, we determined the effect of dieldrin (DD) and chlorpyrifos (CP) on the freshwater crayfish, Cambarellus montezumae. The organisms (4-6cm in diameter) were collected in the Ignacio Ramírez Reservoir, situated at 50km Northeast of Mexico City, in the Rio Lerma Basin. Initially, we determined the LC 50 value with the Probit method, then the DNA damage with single cell gel electrophoresis (comet assay applied at 24, 48, and 72h of exposure) to the brain and hepatopancreas of animals exposed (in reconstituted water) to 0.05 and 0.5 m g/L of each insecticide. In the hepatopancreas of the same organisms, we determined the lipid peroxidation by applying the TBARS test. DNA damage and lipid peroxidation were also evaluated with the same methods to organisms exposed in water from the reservoir. In regard to the LC 50, at 72h of exposure, we found a value of 5.1 m g/L and a value of 5.62 m g/L for DD and CP, respectively. The comet assay applied at different exposure times showed significant DNA damage to both organs, with respect to the control level. In the case of DD, statistical significance was observed for the two doses in the whole evaluated schedule. CP was genotoxic in the brain with the high dose at 72h, and in the hepatopancreas with the two tested doses at all evaluated exposure times. Also, a significant lipid peroxidation increase was detected with the two doses of insecticides. In the study with water from the reservoir, a more pronounced DNA damage was detected. Our results showed strong DNA damage induced by both insecticides in the crayfish, as well as a correlation with the lipid peroxidation effect, suggesting that oxidative stress is involved in the genotoxic alteration. Our results also showed the usefulness of the studied organism as well as the applied tests for the evaluation of toxicological effects, and suggested the pertinence of applying the comet assay to other freshwater organisms to evaluate the bioaccumulation of insecticides. Rev. Biol. Trop. 63 (1): 83-96. Epub 2015 March 01.Universidad de Costa Rica2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdf0034-7744https://www.redalyc.org/articulo.oa?id=44933764008Revista de Biología Tropical (Costa Rica) Num.1 Vol.63reponame:Redalyc-UNAMinstname:Universidad Nacional Autónoma de Méxicoinstacron:UNAMenhttp://www.redalyc.org/revista.oa?id=449Revista de Biología Tropicalinfo:eu-repo/semantics/openAccessoai:redalyc.org:449337640082025-09-03T18:05:52Z
dc.title.none.fl_str_mv Toxicity induced by dieldrin and chlorpyrifos in the freshwater crayfish Cambarellus montezumae (Cambaridae)
title Toxicity induced by dieldrin and chlorpyrifos in the freshwater crayfish Cambarellus montezumae (Cambaridae)
spellingShingle Toxicity induced by dieldrin and chlorpyrifos in the freshwater crayfish Cambarellus montezumae (Cambaridae)
Sandra Díaz-Barriga Arceo
Biología
dieldrin
crayfish
Genotoxicity
chlorpyrifos
lipoperoxidation
title_short Toxicity induced by dieldrin and chlorpyrifos in the freshwater crayfish Cambarellus montezumae (Cambaridae)
title_full Toxicity induced by dieldrin and chlorpyrifos in the freshwater crayfish Cambarellus montezumae (Cambaridae)
title_fullStr Toxicity induced by dieldrin and chlorpyrifos in the freshwater crayfish Cambarellus montezumae (Cambaridae)
title_full_unstemmed Toxicity induced by dieldrin and chlorpyrifos in the freshwater crayfish Cambarellus montezumae (Cambaridae)
title_sort Toxicity induced by dieldrin and chlorpyrifos in the freshwater crayfish Cambarellus montezumae (Cambaridae)
dc.creator.none.fl_str_mv Sandra Díaz-Barriga Arceo
Laura Martínez-Tabche
Isela Álvarez-González
Eugenia López López
Eduardo Madrigal-Bujaidar
author Sandra Díaz-Barriga Arceo
author_facet Sandra Díaz-Barriga Arceo
Laura Martínez-Tabche
Isela Álvarez-González
Eugenia López López
Eduardo Madrigal-Bujaidar
author_role author
author2 Laura Martínez-Tabche
Isela Álvarez-González
Eugenia López López
Eduardo Madrigal-Bujaidar
author2_role author
author
author
author
dc.subject.none.fl_str_mv Biología
dieldrin
crayfish
Genotoxicity
chlorpyrifos
lipoperoxidation
topic Biología
dieldrin
crayfish
Genotoxicity
chlorpyrifos
lipoperoxidation
description The toxicity induced by insecticides in aquatic organisms is of utmost relevance because it may give a clue about the degree of health or damage of the involved ecosystem. In the present report, we determined the effect of dieldrin (DD) and chlorpyrifos (CP) on the freshwater crayfish, Cambarellus montezumae. The organisms (4-6cm in diameter) were collected in the Ignacio Ramírez Reservoir, situated at 50km Northeast of Mexico City, in the Rio Lerma Basin. Initially, we determined the LC 50 value with the Probit method, then the DNA damage with single cell gel electrophoresis (comet assay applied at 24, 48, and 72h of exposure) to the brain and hepatopancreas of animals exposed (in reconstituted water) to 0.05 and 0.5 m g/L of each insecticide. In the hepatopancreas of the same organisms, we determined the lipid peroxidation by applying the TBARS test. DNA damage and lipid peroxidation were also evaluated with the same methods to organisms exposed in water from the reservoir. In regard to the LC 50, at 72h of exposure, we found a value of 5.1 m g/L and a value of 5.62 m g/L for DD and CP, respectively. The comet assay applied at different exposure times showed significant DNA damage to both organs, with respect to the control level. In the case of DD, statistical significance was observed for the two doses in the whole evaluated schedule. CP was genotoxic in the brain with the high dose at 72h, and in the hepatopancreas with the two tested doses at all evaluated exposure times. Also, a significant lipid peroxidation increase was detected with the two doses of insecticides. In the study with water from the reservoir, a more pronounced DNA damage was detected. Our results showed strong DNA damage induced by both insecticides in the crayfish, as well as a correlation with the lipid peroxidation effect, suggesting that oxidative stress is involved in the genotoxic alteration. Our results also showed the usefulness of the studied organism as well as the applied tests for the evaluation of toxicological effects, and suggested the pertinence of applying the comet assay to other freshwater organisms to evaluate the bioaccumulation of insecticides. Rev. Biol. Trop. 63 (1): 83-96. Epub 2015 March 01.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 0034-7744
https://www.redalyc.org/articulo.oa?id=44933764008
identifier_str_mv 0034-7744
url https://www.redalyc.org/articulo.oa?id=44933764008
dc.language.none.fl_str_mv en
language_invalid_str_mv en
dc.relation.none.fl_str_mv http://www.redalyc.org/revista.oa?id=449
dc.rights.none.fl_str_mv Revista de Biología Tropical
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Revista de Biología Tropical
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad de Costa Rica
publisher.none.fl_str_mv Universidad de Costa Rica
dc.source.none.fl_str_mv Revista de Biología Tropical (Costa Rica) Num.1 Vol.63
reponame:Redalyc-UNAM
instname:Universidad Nacional Autónoma de México
instacron:UNAM
instname_str Universidad Nacional Autónoma de México
instacron_str UNAM
institution UNAM
reponame_str Redalyc-UNAM
collection Redalyc-UNAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 14.964248

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