Polyamines as biomarkers of the antitumoral activity of Bursera fagaroides
In normal and tumoral cells, the polyamines (PAs) putrescine(Pu), spermidine (Spd) and spermine (Spm) are required in multiplefundamental cell cycle functions. High levels of PAs havebeen reported in many types of cancer, which is why they wereproposed as biomarkers of cancer growth. In the present...
| Autores: | , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2008 |
| País: | México |
| Institución: | Universidad de Guadalajara |
| Repositorio: | Redalyc-UDG |
| OAI Identifier: | oai:redalyc.org:33933511 |
| Acceso en línea: | https://www.redalyc.org/articulo.oa?id=33933511 |
| Access Level: | acceso abierto |
| Palabra clave: | Multidisciplinarias (Ciencias Sociales) Polyamines Putrescine Murine Lymphoma Cancer Biomarker Bursera fagaroides |
| Sumario: | In normal and tumoral cells, the polyamines (PAs) putrescine(Pu), spermidine (Spd) and spermine (Spm) are required in multiplefundamental cell cycle functions. High levels of PAs havebeen reported in many types of cancer, which is why they wereproposed as biomarkers of cancer growth. In the present work,their utility as biomarkers of the evolution of the murine L5178Ylymphoma is reported in different body fluids, cells and tissues.Findings were also applied to the follow-up of the previously reportedanti-tumor effect of Bursera fagaroides. Cation exchangechromatography was used to determine the PAs levels in urine,peritoneal cells, circulating lymphocytes, spleenocytes, mesotheliumand liver of BALB/c mice at days 10, 17 and 24 of tumoralevolution. PAs levels were also measured in urine from micetreated, intraperitoneally or orally, with the hydroalcoholic extractof the bark of B. fagaroides. Spd and Spm urinary levels were notdetectable, while Pu increase in urine is the best biomarker to detectlymphoma growth. Furthermore, Pu urinary levels decreasedsignificantly in mice treated intraperitoneally with B. fagaroides.In this model, variations of the Pu urinary level is an effectivebiomarker of neoplastic growth as it allows to follow the evolutionof L5178Y lymphoma, providing an in vivo assay for the antitumoraleffect of B. fagaroides and other drugs. |
|---|