Thidiazuron-induced shoot organogenesis of Cleome viscosa (L) through cotyledonary explants culture
A reproducible protocol for direct shoot organogenesis of Cleome viscosa, an important medicinal weed herb was developed. The seed explants were collected primarily from field grown mature plants for in vitro germination on different strength of MS basal medium with or without selection of GA3 at va...
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2014 |
| País: | México |
| Institución: | Centro de Investigación Científica de Yucatán |
| Repositorio: | Repositorio Institucional CICY |
| Idioma: | inglés |
| OAI Identifier: | oai:cicy.repositorioinstitucional.mx:1003/724 |
| Acceso en línea: | http://cicy.repositorioinstitucional.mx/jspui/handle/1003/724 |
| Access Level: | acceso abierto |
| Palabra clave: | info:eu-repo/classification/Autores/CLEOME VISCOSA L info:eu-repo/classification/Autores/MEDICINAL WEED PLANT info:eu-repo/classification/Autores/SHOOT MULTIPLICATION info:eu-repo/classification/Autores/DIRECT ORGANOGENESIS info:eu-repo/classification/cti/2 |
| Sumario: | A reproducible protocol for direct shoot organogenesis of Cleome viscosa, an important medicinal weed herb was developed. The seed explants were collected primarily from field grown mature plants for in vitro germination on different strength of MS basal medium with or without selection of GA3 at various concentration (0.1 to 1.0 mg/L). The highest rate of seed germination (55.3%) was noticed on full strength MS basal medium fortified with 0.5 mg/L GA3 after 30 days of culture. The excised 7 to 10 days old cotyledonary leaf, cotyledonary node and hypocotyls explants cultured on MS medium fortified with different concentration of individual cytokinin (BA/KIN/TDZ) alone or BA+KIN or TDZ+KIN or TDZ in combinations with different auxins (IBA/NAA/IAA) influenced the frequency of adventitious microshoot formation. |
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