Optimización de un protocolo del aislamiento del ADN y de un sistema de amplificación ISSR-PCR para Ceratozamia mexicana Brongn. (Zamiaceae).
Most of the cycads contain high concentrations of essential oils, flavonoids, polyphenols, and polysaccharides that interfere with DNA extraction, causing erroneous or no PCR products. The optimization of DNA isolation, employing inter-simple sequence repeats (ISSRs) primers were investigated in Cer...
| Autores: | , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2012 |
| País: | México |
| Institución: | Centro de Investigación Científica de Yucatán |
| Repositorio: | Repositorio Institucional CICY |
| Idioma: | español |
| OAI Identifier: | oai:cicy.repositorioinstitucional.mx:1003/1264 |
| Acceso en línea: | http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1264 |
| Access Level: | acceso abierto |
| Palabra clave: | info:eu-repo/classification/Autores/CERATOZAMIA MEXICANA info:eu-repo/classification/Autores/CICADAS info:eu-repo/classification/Autores/EXTRACCION DE ADN info:eu-repo/classification/Autores/ESPECIES EN PELIGRO info:eu-repo/classification/Autores/ISSR info:eu-repo/classification/cti/2 |
| id |
MX_e8aad8cf5d35d1c1cf4281d9ee06ecb8 |
|---|---|
| oai_identifier_str |
oai:cicy.repositorioinstitucional.mx:1003/1264 |
| network_acronym_str |
MX |
| network_name_str |
México |
| repository_id_str |
|
| spelling |
Optimización de un protocolo del aislamiento del ADN y de un sistema de amplificación ISSR-PCR para Ceratozamia mexicana Brongn. (Zamiaceae).Optimization of a protocol for DNA isolation and ISSR-PCR amplification system for Ceratozamia mexicana Brongn. (Zamiaceae).NADIA GUADALUPE SANCHEZ COELLOMAURICIO LUNA RODRIGUEZSANTIAGO MARIO VAZQUEZ TORRESLAZARO RAFAEL SANCHEZ VELASQUEZNANCY SANTANA BUZZYPABLO OCTAVIO AGUILARLOURDES GEORGINA IGLESIAS ANDREUinfo:eu-repo/classification/Autores/CERATOZAMIA MEXICANAinfo:eu-repo/classification/Autores/CICADASinfo:eu-repo/classification/Autores/EXTRACCION DE ADNinfo:eu-repo/classification/Autores/ESPECIES EN PELIGROinfo:eu-repo/classification/Autores/ISSRinfo:eu-repo/classification/cti/2info:eu-repo/classification/cti/2Most of the cycads contain high concentrations of essential oils, flavonoids, polyphenols, and polysaccharides that interfere with DNA extraction, causing erroneous or no PCR products. The optimization of DNA isolation, employing inter-simple sequence repeats (ISSRs) primers were investigated in Ceratozamia mexicana Brongn., an endangered Mexican cycad. The DNA obtained from fresh-leaf tissues with a modified cetyltrimethylammonium bromide buffer protocol gave a good quality of DNA with no colored pigments and contaminants. The main modification to the CTAB-based DNA extraction protocol was the one hour leaf tissue soaking pre-treatment with a 0.7 M NaCl solution, to facilitate the cell lysis. The DNA extracted was successfully amplified by PCR using six arbitrary ISSR primers. Reproducible amplifiable products were observed in all PCR reactions. Our results show a significant improvement in the DNA quality obtained using low primer concentration (25 pM). 23 strong bands were detected, 9 of which were polymorphic. The results indicated that the optimized protocol for DNA isolation and PCR system is suitable for further work in this specie. This work is the first DNA extraction and ISSR protocols reported for this ornamental and endangered species.2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://cicy.repositorioinstitucional.mx/jspui/handle/1003/1264Revista Chapingo Serie Ciencias Forestales y del Ambiente, 18(1), 123-133, 2012.reponame:Repositorio Institucional CICYinstname:Centro de Investigación Científica de Yucatáninstacron:CICYspainfo:eu-repo/semantics/datasetDOI/http://dx.doi.org/10.5154/r.rchscfa.2011.03.024citation:Sánchez-Coello, N. G., Luna-Rodríguez, M., Vázquez-Torres, S. M., Sánchez-Velásquez, L. R., Santana-Buzzy, N., Octavio-Aguilar, P., & Iglesias-Andreu, L. G. (2012). Optimization of a protocol for DNA isolation and ISSR-PCR amplification system for Ceratozamia mexicana Brongn. (Zamiaceae). Revista Chapingo Serie Ciencias Forestales y del Ambiente, 18(1), 123-133.info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0oai:cicy.repositorioinstitucional.mx:1003/12642024-08-28T03:18:38Z |
| dc.title.none.fl_str_mv |
Optimización de un protocolo del aislamiento del ADN y de un sistema de amplificación ISSR-PCR para Ceratozamia mexicana Brongn. (Zamiaceae). Optimization of a protocol for DNA isolation and ISSR-PCR amplification system for Ceratozamia mexicana Brongn. (Zamiaceae). |
| title |
Optimización de un protocolo del aislamiento del ADN y de un sistema de amplificación ISSR-PCR para Ceratozamia mexicana Brongn. (Zamiaceae). |
| spellingShingle |
Optimización de un protocolo del aislamiento del ADN y de un sistema de amplificación ISSR-PCR para Ceratozamia mexicana Brongn. (Zamiaceae). NADIA GUADALUPE SANCHEZ COELLO info:eu-repo/classification/Autores/CERATOZAMIA MEXICANA info:eu-repo/classification/Autores/CICADAS info:eu-repo/classification/Autores/EXTRACCION DE ADN info:eu-repo/classification/Autores/ESPECIES EN PELIGRO info:eu-repo/classification/Autores/ISSR info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 |
| title_short |
Optimización de un protocolo del aislamiento del ADN y de un sistema de amplificación ISSR-PCR para Ceratozamia mexicana Brongn. (Zamiaceae). |
| title_full |
Optimización de un protocolo del aislamiento del ADN y de un sistema de amplificación ISSR-PCR para Ceratozamia mexicana Brongn. (Zamiaceae). |
| title_fullStr |
Optimización de un protocolo del aislamiento del ADN y de un sistema de amplificación ISSR-PCR para Ceratozamia mexicana Brongn. (Zamiaceae). |
| title_full_unstemmed |
Optimización de un protocolo del aislamiento del ADN y de un sistema de amplificación ISSR-PCR para Ceratozamia mexicana Brongn. (Zamiaceae). |
| title_sort |
Optimización de un protocolo del aislamiento del ADN y de un sistema de amplificación ISSR-PCR para Ceratozamia mexicana Brongn. (Zamiaceae). |
| dc.creator.none.fl_str_mv |
NADIA GUADALUPE SANCHEZ COELLO MAURICIO LUNA RODRIGUEZ SANTIAGO MARIO VAZQUEZ TORRES LAZARO RAFAEL SANCHEZ VELASQUEZ NANCY SANTANA BUZZY PABLO OCTAVIO AGUILAR LOURDES GEORGINA IGLESIAS ANDREU |
| author |
NADIA GUADALUPE SANCHEZ COELLO |
| author_facet |
NADIA GUADALUPE SANCHEZ COELLO MAURICIO LUNA RODRIGUEZ SANTIAGO MARIO VAZQUEZ TORRES LAZARO RAFAEL SANCHEZ VELASQUEZ NANCY SANTANA BUZZY PABLO OCTAVIO AGUILAR LOURDES GEORGINA IGLESIAS ANDREU |
| author_role |
author |
| author2 |
MAURICIO LUNA RODRIGUEZ SANTIAGO MARIO VAZQUEZ TORRES LAZARO RAFAEL SANCHEZ VELASQUEZ NANCY SANTANA BUZZY PABLO OCTAVIO AGUILAR LOURDES GEORGINA IGLESIAS ANDREU |
| author2_role |
author author author author author author |
| dc.subject.none.fl_str_mv |
info:eu-repo/classification/Autores/CERATOZAMIA MEXICANA info:eu-repo/classification/Autores/CICADAS info:eu-repo/classification/Autores/EXTRACCION DE ADN info:eu-repo/classification/Autores/ESPECIES EN PELIGRO info:eu-repo/classification/Autores/ISSR info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 |
| topic |
info:eu-repo/classification/Autores/CERATOZAMIA MEXICANA info:eu-repo/classification/Autores/CICADAS info:eu-repo/classification/Autores/EXTRACCION DE ADN info:eu-repo/classification/Autores/ESPECIES EN PELIGRO info:eu-repo/classification/Autores/ISSR info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 |
| description |
Most of the cycads contain high concentrations of essential oils, flavonoids, polyphenols, and polysaccharides that interfere with DNA extraction, causing erroneous or no PCR products. The optimization of DNA isolation, employing inter-simple sequence repeats (ISSRs) primers were investigated in Ceratozamia mexicana Brongn., an endangered Mexican cycad. The DNA obtained from fresh-leaf tissues with a modified cetyltrimethylammonium bromide buffer protocol gave a good quality of DNA with no colored pigments and contaminants. The main modification to the CTAB-based DNA extraction protocol was the one hour leaf tissue soaking pre-treatment with a 0.7 M NaCl solution, to facilitate the cell lysis. The DNA extracted was successfully amplified by PCR using six arbitrary ISSR primers. Reproducible amplifiable products were observed in all PCR reactions. Our results show a significant improvement in the DNA quality obtained using low primer concentration (25 pM). 23 strong bands were detected, 9 of which were polymorphic. The results indicated that the optimized protocol for DNA isolation and PCR system is suitable for further work in this specie. This work is the first DNA extraction and ISSR protocols reported for this ornamental and endangered species. |
| publishDate |
2012 |
| dc.date.none.fl_str_mv |
2012 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1264 |
| url |
http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1264 |
| dc.language.none.fl_str_mv |
spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
info:eu-repo/semantics/datasetDOI/http://dx.doi.org/10.5154/r.rchscfa.2011.03.024 citation:Sánchez-Coello, N. G., Luna-Rodríguez, M., Vázquez-Torres, S. M., Sánchez-Velásquez, L. R., Santana-Buzzy, N., Octavio-Aguilar, P., & Iglesias-Andreu, L. G. (2012). Optimization of a protocol for DNA isolation and ISSR-PCR amplification system for Ceratozamia mexicana Brongn. (Zamiaceae). Revista Chapingo Serie Ciencias Forestales y del Ambiente, 18(1), 123-133. |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0 |
| eu_rights_str_mv |
openAccess |
| rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0 |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.source.none.fl_str_mv |
Revista Chapingo Serie Ciencias Forestales y del Ambiente, 18(1), 123-133, 2012. reponame:Repositorio Institucional CICY instname:Centro de Investigación Científica de Yucatán instacron:CICY |
| instname_str |
Centro de Investigación Científica de Yucatán |
| instacron_str |
CICY |
| institution |
CICY |
| reponame_str |
Repositorio Institucional CICY |
| collection |
Repositorio Institucional CICY |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1858177483587715072 |
| score |
15,811543 |