Efectos de lipopolisacárido y acido lipoteicoico sobre la ciclooxigenasa-2 en celulas pulpares humanas
Bacterial toxins such as lipopolysaccharide (LPS) and lipoteichoic acid (LTA) can be found in Gram-negative and Gram-positive bacteria, respectively. This toxins cause an inflammatory process denominated pulpitis. In the present study, the effect of LTA and LPS on the expression of cyclooxygenase-2...
| Authors: | , , |
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| Format: | article |
| Status: | Published version |
| Publication Date: | 2022 |
| Country: | México |
| Institution: | UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO |
| Repository: | Revista Odontológica Mexicana |
| Language: | Spanish |
| OAI Identifier: | oai:ojs.pkp.sfu.ca:article/15625 |
| Online Access: | https://revistas.unam.mx/index.php/rom/article/view/15625 |
| Access Level: | Open access |
| Keyword: | LTA lypoteichoic acid LPS lipopolysaccharide COX-2 cyclooxygenase-2 PGE2 prostaglandin E2 ERK extracellular signalregulated protein kinases PKC protein kinases C JNK C-Jun NH2-terminal kinase Ácido lipoteicoico lipopolisacárido ciclooxigenasa prostaglandina E2 proteína cinasa regulada extracelularmente proteína cinasa C JKN cinasa aminoterminal reguladora del factor de transcripción C-Jun milimolar (mM) |
| Summary: | Bacterial toxins such as lipopolysaccharide (LPS) and lipoteichoic acid (LTA) can be found in Gram-negative and Gram-positive bacteria, respectively. This toxins cause an inflammatory process denominated pulpitis. In the present study, the effect of LTA and LPS on the expression of cyclooxygenase-2 in human dental pulp cells was investigated. For this study a hypothesis was made, that this molecules take part in the dental pulp inflammatory process through the cyclooxygenase-2 synthesis in human pulp cells. To evaluate this hypothesis the effect of LPS and LTA was studied in primary cultures of human pulp cells. Finally, the pathways and molecules involved in COX-2 expression were characterized. In human dental pulp cells treated with LTA or LPS, it was detected that these molecules promote COX-2 expression. On the other hand, it was founded that the expression of COX-2 in human pulp cells was blocked by treatments with SB203580, PD98059, and Estaurosporine. The results of the present study demonstrate that treatments with LTA and LPS promote COX-2 expression in events related with protein kinase p38, ERK and PKC. The findings of this investigation are important because COX-2 is related to the synthesis of PGE2, which leads to a chronic inflammatory process. Finally, the nature of a mediator implied in the pulpitis process was characterized. |
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