Influencia de la asociación endófita entre Acacia farnesiana y Methylobacterium oryzae en la tolerancia y bioacumulación de arsénico
Arsenic (As) is a metalloid highly toxic and widely distributed in the ground. For that reason, nowadays there are several technologies to clean As-polluted soils. One of these is phytoremediation, which use tolerant plant and its associated microorganisms (MOs), which can modify the ability of plan...
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| Tipo de documento: | tese |
| Estado: | Versão publicada |
| Data de publicação: | 2018 |
| País: | México |
| Recursos: | Universidad Autónoma Metropolitana |
| Repositório: | Repositorio Institucional de la UAM Iztapalapa |
| Idioma: | espanhol |
| OAI Identifier: | oai:bindani.izt.uam.mx:m039k489b |
| Acesso em linha: | https://doi.org/10.24275/uami.m039k489b |
| Access Level: | Acceso aberto |
| Palavra-chave: | info:eu-repo/classification/LEM/Symbiosis info:eu-repo/classification/LEM/Arsénico info:eu-repo/classification/LEM/Phytoremediation info:eu-repo/classification/LEM/Oxidant stress info:eu-repo/classification/LEM/fitorremediación info:eu-repo/classification/LEM/Arsenic info:eu-repo/classification/LEM/Estrés oxidante info:eu-repo/classification/LEM/Simbiosis info:eu-repo/classification/LEM/Methylobacterium oryzae info:eu-repo/classification/LEM/Acacia farnesiana info:eu-repo/classification/cti/6 |
| Resumo: | Arsenic (As) is a metalloid highly toxic and widely distributed in the ground. For that reason, nowadays there are several technologies to clean As-polluted soils. One of these is phytoremediation, which use tolerant plant and its associated microorganisms (MOs), which can modify the ability of plant to bioaccumulate toxic elements in their tissue. For that, growth of a plant tolerant to As, Acacia farnesiana, was tested in presence of different MOs, under As stress conditions. From results of biomass and bioaccumulation of As in stem and roots, an endophytic bacteria identified as Methylobacterium oryzae was selected to perform phytoremediation assays. One way to understand intracellular mechanisms involved in As-plant tolerance as well as the effect of plant-MO symbiosis is through the quantification of the enzymatic activity and presence of molecules involved in such a mechanisms and the analysis of proteomic profile. Thus, the aim of this work was to evaluate the influence of the endophytic M. oryzae in the As-tolerance of A farnesiana through the analysis of the As bioaccumulated, physiological changes as well as differential protein regulation. For that, A. farnesiana and A. farnesiana- M. oryzae plants were grown in vitro, with 0 and 580 µM As for 15 days. The presence of bacteria did not modify the vegetal growth despite the increase of As bioaccumulation in shoots and roots and the increase of chlorophyll. Symbiosis also increased glutathione (GSH) content, as well as GSH/GSSG rate, decreasing the oxidation degree (OD), in both shoots and roots. On the other hand, symbiosis decreased the H2O2 level induced by As in roots, as well as GST and POX activity in both tissues of As-stressed plants, indicating a general improvement in the redox cellular state. The obtained results suggest a protective effect of symbiosis against the As toxicity, which favor the vegetal tolerance. The changes induced by symbiosis could be regulated by modifications in the intracellular proteome. The proteomic analysis demonstrated that M. oryzae alleviated the Asinduced metabolic disorder, mainly in shoot where bacteria positively regulated proteins related to carbohydrate metabolism, aminoacid metabolism, GSH biosynthesis and protein folding, whereas in roots, M. oryzae increased the diversity of protein isoforms expressed and maintained the abundance of proteins related to metabolism of aminoacids in response to the high As bioaccumulated. Keywords: Acacia farnesiana, Methylobacterium oryzae, symbiosis, phytoremediation, arsenic, GST, oxidant stress, proteome |
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