Allelic variations of Puroindoline a and Puroindoline b genes in new type of synthetic hexaploid wheats from CIMMYT

Triticum dicoccoides and Aegilops tauschii are important germplasm resources of rich genetic variability for improvement of hexaploid wheat (T. aestivum). This variability can be accessed by utilizing synthetic hexaploid wheat lines with genomes from Ae. tauschii and T. dicoccoides. Two methods can...

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Autores: Genying Li, Xianchun Xia, He Zhonghu, Sun, Qi-Xin
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2007
País:México
Institución:Centro Internacional de Mejoramiento de Maíz y Trigo
Repositorio:Repositorio Institucional de Publicaciones Multimedia del CIMMYT
OAI Identifier:oai:repository.cimmyt.org:10883/21409
Acceso en línea:https://hdl.handle.net/10883/21409
Access Level:acceso abierto
Palabra clave:AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
Triticum dicoccoides
Aegilops tauschii
Synthetic Hexaploid Wheat
Pin a
Pin b
Kernel Texture
WHEAT
AEGILOPS
HEXAPLOIDY
PLANT BIOTECHNOLOGY
ALLELES
KERNELS
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oai_identifier_str oai:repository.cimmyt.org:10883/21409
network_acronym_str MX
network_name_str México
repository_id_str
dc.title.none.fl_str_mv Allelic variations of Puroindoline a and Puroindoline b genes in new type of synthetic hexaploid wheats from CIMMYT
title Allelic variations of Puroindoline a and Puroindoline b genes in new type of synthetic hexaploid wheats from CIMMYT
spellingShingle Allelic variations of Puroindoline a and Puroindoline b genes in new type of synthetic hexaploid wheats from CIMMYT
Genying Li
AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
Triticum dicoccoides
Aegilops tauschii
Synthetic Hexaploid Wheat
Pin a
Pin b
Kernel Texture
WHEAT
AEGILOPS
HEXAPLOIDY
PLANT BIOTECHNOLOGY
ALLELES
KERNELS
title_short Allelic variations of Puroindoline a and Puroindoline b genes in new type of synthetic hexaploid wheats from CIMMYT
title_full Allelic variations of Puroindoline a and Puroindoline b genes in new type of synthetic hexaploid wheats from CIMMYT
title_fullStr Allelic variations of Puroindoline a and Puroindoline b genes in new type of synthetic hexaploid wheats from CIMMYT
title_full_unstemmed Allelic variations of Puroindoline a and Puroindoline b genes in new type of synthetic hexaploid wheats from CIMMYT
title_sort Allelic variations of Puroindoline a and Puroindoline b genes in new type of synthetic hexaploid wheats from CIMMYT
dc.creator.none.fl_str_mv Genying Li
Xianchun Xia
He Zhonghu
Sun, Qi-Xin
author Genying Li
author_facet Genying Li
Xianchun Xia
He Zhonghu
Sun, Qi-Xin
author_role author
author2 Xianchun Xia
He Zhonghu
Sun, Qi-Xin
author2_role author
author
author
dc.subject.none.fl_str_mv AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
Triticum dicoccoides
Aegilops tauschii
Synthetic Hexaploid Wheat
Pin a
Pin b
Kernel Texture
WHEAT
AEGILOPS
HEXAPLOIDY
PLANT BIOTECHNOLOGY
ALLELES
KERNELS
topic AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
Triticum dicoccoides
Aegilops tauschii
Synthetic Hexaploid Wheat
Pin a
Pin b
Kernel Texture
WHEAT
AEGILOPS
HEXAPLOIDY
PLANT BIOTECHNOLOGY
ALLELES
KERNELS
description Triticum dicoccoides and Aegilops tauschii are important germplasm resources of rich genetic variability for improvement of hexaploid wheat (T. aestivum). This variability can be accessed by utilizing synthetic hexaploid wheat lines with genomes from Ae. tauschii and T. dicoccoides. Two methods can be employed to incorporate Triticum dicoccoides and Aegilops tauschii genes into a hexaploid wheat. One is the direct cross method of hybridization between Ae. tauschii and hexaploid wheat, another is an indirect method of synthetic hexaploids by crossing tetraploid wheat with Ae. tauschii. Genes from tetraploid wheat and Aegilops tauschii are then available via direct crossing of synthetic hexaploids to T. aestivum. The CIMMYT (International Maize and Wheat Improvement Center) has produced two types of synthetic hexaploids. One is the progeny of T. turgidum ssp. durum crossing with Ae. tauschii, another is the new type synthetic wheat which produced by crossing Triticum dicoccoides with Aegilops tauschii. Grain hardness is a major factor influencing the end-use quality. Genes for grain hardness in common wheat reside on Ha locus of chromosome 5DS. The wild-type alleles (Pina-D1a, Pinb-D1a) determine soft endosperm, while the hard phenotypes result from mutations in either Pina or Pinb. In order to understand the evolution of genes for grain hardness, studies have focused on the wild relatives of common wheat, particularly on Ae. tauschii, the supposed donor of the D genome in common wheat. Puroindoline a in Ae. tauschii contained 99.3% amino acid sequence homology to the wheat cultivar ‘Penawawa’ and 90.5% amino acid sequence homology in puroindoline b. Among 50 Ae. tauschii accessions, four alleles of puroindoline a and four alleles of puroindoline b were identified, encoding two and three different proteins, respectively. However, the effect that these sequence polymorphisms may have on kernel texture is unknown, as no test method of texture has been developed for Ae. tauschii. The incorporation of Ae. tauschii into synthetic hexaploid facilitates the analysis of puroindoline sequence polymorphism and other genetic effects on kernel texture, using testing methods designed for hexaploid wheat, such as the Perten single kernel characterization system (SKCS). Analysis of synthetic hexaploid can also facilitate the analysis of the effect of the A and B genomes, contributed by the tetraploid wheat parent on kernel texture. Studies on Ae. tauschii and synthetic hexaploid wheat derived from hybridization of T. turgidum ssp. durum with Ae. tauschii demonstrated a number of novel Pina and Pinb sequences. However, none of these Pina and Pinb mutations produced a hard endosperm texture in synthetic wheat. To further understand the mechanism of grain hardness formation in Ae. tauschii and synthetic wheat, a total of 57 new type synthetic hexaploid lines derived from the crosses T. dicoccoides×Ae.tauschii were employed to evaluate the SKCS hardness and detect puroindoline alleles using gene specific PCR primers. Four allelic variations of Pina and Pinb were identified, i.e., Pina-D1a, Pina-D1c, Pinb-D1h, and Pinb-D1j. Eight lines were Pina-D1a/Pinb-D1j types, accounting for 14%, and 49 were Pina-D1c/Pinb-D1h types, accounting for 86%. Kernel texture differed significantly among the synthetic hexaploid lines, ranging from 10.5 to 42.6, with significant difference among the parental types, T. dicoccoides or Ae. tauschii. The interactions between parental genotypes had also a significant influence on kernel texture. These results indicated that there might be some genes besides Ha locus associated with the kernel texture.
publishDate 2007
dc.date.none.fl_str_mv 2007
2021-04-19T20:27:31Z
2021-04-19T20:27:31Z
dc.type.none.fl_str_mv Published Version
info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/10883/21409
url https://hdl.handle.net/10883/21409
dc.language.none.fl_str_mv Chinese
language_invalid_str_mv Chinese
dc.rights.none.fl_str_mv Open Access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Open Access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Beijing, (China)
dc.publisher.none.fl_str_mv Institute of Crop Sciences
http://zwxb.chinacrops.org/EN/abstract/abstract2118.shtml
publisher.none.fl_str_mv Institute of Crop Sciences
http://zwxb.chinacrops.org/EN/abstract/abstract2118.shtml
dc.source.none.fl_str_mv 2
33
0496-3490
Acta Agronomica Sinica
reponame:Repositorio Institucional de Publicaciones Multimedia del CIMMYT
instname:Centro Internacional de Mejoramiento de Maíz y Trigo
instacron:CIMMYT
instname_str Centro Internacional de Mejoramiento de Maíz y Trigo
instacron_str CIMMYT
institution CIMMYT
reponame_str Repositorio Institucional de Publicaciones Multimedia del CIMMYT
collection Repositorio Institucional de Publicaciones Multimedia del CIMMYT
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Allelic variations of Puroindoline a and Puroindoline b genes in new type of synthetic hexaploid wheats from CIMMYTGenying LiXianchun XiaHe ZhonghuSun, Qi-XinAGRICULTURAL SCIENCES AND BIOTECHNOLOGYTriticum dicoccoidesAegilops tauschiiSynthetic Hexaploid WheatPin aPin bKernel TextureWHEATAEGILOPSHEXAPLOIDYPLANT BIOTECHNOLOGYALLELESKERNELSTriticum dicoccoides and Aegilops tauschii are important germplasm resources of rich genetic variability for improvement of hexaploid wheat (T. aestivum). This variability can be accessed by utilizing synthetic hexaploid wheat lines with genomes from Ae. tauschii and T. dicoccoides. Two methods can be employed to incorporate Triticum dicoccoides and Aegilops tauschii genes into a hexaploid wheat. One is the direct cross method of hybridization between Ae. tauschii and hexaploid wheat, another is an indirect method of synthetic hexaploids by crossing tetraploid wheat with Ae. tauschii. Genes from tetraploid wheat and Aegilops tauschii are then available via direct crossing of synthetic hexaploids to T. aestivum. The CIMMYT (International Maize and Wheat Improvement Center) has produced two types of synthetic hexaploids. One is the progeny of T. turgidum ssp. durum crossing with Ae. tauschii, another is the new type synthetic wheat which produced by crossing Triticum dicoccoides with Aegilops tauschii. Grain hardness is a major factor influencing the end-use quality. Genes for grain hardness in common wheat reside on Ha locus of chromosome 5DS. The wild-type alleles (Pina-D1a, Pinb-D1a) determine soft endosperm, while the hard phenotypes result from mutations in either Pina or Pinb. In order to understand the evolution of genes for grain hardness, studies have focused on the wild relatives of common wheat, particularly on Ae. tauschii, the supposed donor of the D genome in common wheat. Puroindoline a in Ae. tauschii contained 99.3% amino acid sequence homology to the wheat cultivar ‘Penawawa’ and 90.5% amino acid sequence homology in puroindoline b. Among 50 Ae. tauschii accessions, four alleles of puroindoline a and four alleles of puroindoline b were identified, encoding two and three different proteins, respectively. However, the effect that these sequence polymorphisms may have on kernel texture is unknown, as no test method of texture has been developed for Ae. tauschii. The incorporation of Ae. tauschii into synthetic hexaploid facilitates the analysis of puroindoline sequence polymorphism and other genetic effects on kernel texture, using testing methods designed for hexaploid wheat, such as the Perten single kernel characterization system (SKCS). Analysis of synthetic hexaploid can also facilitate the analysis of the effect of the A and B genomes, contributed by the tetraploid wheat parent on kernel texture. Studies on Ae. tauschii and synthetic hexaploid wheat derived from hybridization of T. turgidum ssp. durum with Ae. tauschii demonstrated a number of novel Pina and Pinb sequences. However, none of these Pina and Pinb mutations produced a hard endosperm texture in synthetic wheat. To further understand the mechanism of grain hardness formation in Ae. tauschii and synthetic wheat, a total of 57 new type synthetic hexaploid lines derived from the crosses T. dicoccoides×Ae.tauschii were employed to evaluate the SKCS hardness and detect puroindoline alleles using gene specific PCR primers. Four allelic variations of Pina and Pinb were identified, i.e., Pina-D1a, Pina-D1c, Pinb-D1h, and Pinb-D1j. Eight lines were Pina-D1a/Pinb-D1j types, accounting for 14%, and 49 were Pina-D1c/Pinb-D1h types, accounting for 86%. Kernel texture differed significantly among the synthetic hexaploid lines, ranging from 10.5 to 42.6, with significant difference among the parental types, T. dicoccoides or Ae. tauschii. The interactions between parental genotypes had also a significant influence on kernel texture. These results indicated that there might be some genes besides Ha locus associated with the kernel texture.242-249Institute of Crop Scienceshttp://zwxb.chinacrops.org/EN/abstract/abstract2118.shtml2021-04-19T20:27:31Z2021-04-19T20:27:31Z2007Published Versioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10883/214092330496-3490Acta Agronomica Sinicareponame:Repositorio Institucional de Publicaciones Multimedia del CIMMYTinstname:Centro Internacional de Mejoramiento de Maíz y Trigoinstacron:CIMMYTChineseBeijing, (China)CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose.Open Accessinfo:eu-repo/semantics/openAccessoai:repository.cimmyt.org:10883/214092024-10-11T19:58:08Z
score 15,81155

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