Modificación enzimática de la goma de mezquite para la obtención de L-arabinosa
In this work a study about production of a biocatalyst that is responsible of the hydrolysis of mesquite gum to obtaining L-arabinose released and emulsifies agents was carried out. Production of crude enzymatic extracts obtained from Aspergillus niger has been reported for the degradation of arabin...
| Autor: | |
|---|---|
| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2007 |
| País: | México |
| Institución: | Universidad Autónoma Metropolitana |
| Repositorio: | Repositorio Institucional de la UAM Iztapalapa |
| Idioma: | español |
| OAI Identifier: | oai:bindani.izt.uam.mx:5q47rp03r |
| Acceso en línea: | https://doi.org/10.24275/uami.5q47rp03r |
| Access Level: | acceso abierto |
| Palabra clave: | info:eu-repo/classification/LEM/Biotecnología info:eu-repo/classification/LEM/L-Arabinosa info:eu-repo/classification/LEM/Goma de mezquite info:eu-repo/classification/LEM/Enzymes info:eu-repo/classification/LEM/Enzimas info:eu-repo/classification/LEM/Biotechnology info:eu-repo/classification/cti/6 |
| Sumario: | In this work a study about production of a biocatalyst that is responsible of the hydrolysis of mesquite gum to obtaining L-arabinose released and emulsifies agents was carried out. Production of crude enzymatic extracts obtained from Aspergillus niger has been reported for the degradation of arabinofuranosyl-glucosides, arabinans, arabinoxylans, etc. Growth of A. niger is well known in both submerged and solid stated fermentations. Growth parameters of A. niger 10 obtained were Xmax = 3.03 g L-1 and μmax = 0.07 h-1. α-L-Arabinofuranosidase (EC 3.2.1.55) is responsible of the hydrolysis attributable to the cleavage of the 1Æ3 and 1Æ5 bonds of the L-arabinose in distinct polysaccharides. Maximum enzymatic activity obtained was 65.93 U L-1. Optimal temperature and activation energy for the crude extract were 50 °C and 46.15 KJ mol-1 and for the commercial enzyme 40 °C and 52.76 KJ mol-1, respectively. Apparent kinetic parameters for the crude extract were compared with those obtained for a commercial enzyme over the mesquite gum. The Km-app and Vmax-app values to the crude extract were 4.87 g L-1 and 0.15 μmol min-1 g -1, and to commercial enzyme were 76.45 g L-1 and 3.85 μmol min-1 g-1, respectively. Yields of L-arabinose recovery for the crude extract and the commercial enzyme were 17.04 % and 2.78 %, respectively, based on the reported average content of L-arabinose in mesquite gum. Mesquite gum pretreatment with 24 h of enzymatic hydrolysis and mesquite gum without hydrolysis were fractioned by hydrophobic interaction column. Three fractions were obtaining in both cases and the sugar and protein contents were determinate to evaluate indirectly the hydrophilic-lipophilic balance. Mesquite gum pretreatment with 24 h of enzymatic hydrolysis and first fraction of mesquite gum pretreatment with 24 h of enzymatic hydrolysis and second fractions of mesquite gum without hydrolysis and with hydrolysis could be useful like emulsifier agents. In conclusion the use of mesquite gum to release L-arabinose via enzymatic is possible and at the same time is possible the obtaining of emulsifier agents with add high value. |
|---|