Promoter paper Pron1o ter analysis of the acetate-inducible isocitrate lyase gene (acu-3) from fleurospora crassa
Analysis of the promoter region of the clcetate-inclucecl isocitr tte ly:J>c gene (ocu-3) of Neurospora crus.w w:Js undert:tkcn. A series of deletions in the 5' non-tr tnscribed region were constructed :mel the effects of these mutation s on the enzyme levels following gro\\'th on sucro...
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2012 |
| País: | México |
| Recursos: | Instituto Politécnico Nacional |
| Repositorio: | Repositorio Digital del IPN |
| OAI Identifier: | oai:www.repositoriodigital.ipn.mx:123456789/8547 |
| Acesso em linha: | http://www.repositoriodigital.ipn.mx/handle/123456789/8547 |
| Access Level: | acceso abierto |
| Palavra-chave: | fleurospora crassa |
| Resumo: | Analysis of the promoter region of the clcetate-inclucecl isocitr tte ly:J>c gene (ocu-3) of Neurospora crus.w w:Js undert:tkcn. A series of deletions in the 5' non-tr tnscribed region were constructed :mel the effects of these mutation s on the enzyme levels following gro\\'th on sucrose and transfer to ncetale \\'ere measured. Sc:quences wtthin the region -603 to -271 n.:lati\c to the transcription start s ite appear essential for transcription. The region -950 IO - 127S is requir cl for sucrose repression. which i<> con istent with prninw; protein/DNA gel rd<lrtl<ttic>n results of rrotcin extr:1cts from .V. cms.m o::u!tured c!!l sucrc:s:::. Protein extracts l'rom acetate-induced mycelia identify alternative promoter regions apparently im•o!ved in acetat<>inducecl gene transcription. •(l 199R Elsevier Science B.V. All rights reserved. |
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