Fibrillarin ribonuclease activity is dependent on the GAR domain and modulated by phospholipids

Fibrillarin is a highly conserved nucleolar methyltransferase responsible for ribosomal RNA methylation across evolution from Archaea to humans. It has been reported that fibrillarin is involved in the methylation of histone H2A in nucleoli and other processes, including viral progression, cellular...

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Detalles Bibliográficos
Autores: Francisco Guillén-Chable, ULISES RODRIGUEZ CORONA, Alejandro Pereira-Santana, ANDREA BAYONA HERNANDEZ, Luis Carlos Rodríguez Zapata, Cecilia Aquino Perez, Lenka Šebestová, Nicolas Vitale, Pavel Hozak, Enrique Castaño de la Serna
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:México
Institución:Centro de Investigación Científica de Yucatán
Repositorio:Repositorio Institucional CICY
Idioma:inglés
OAI Identifier:oai:cicy.repositorioinstitucional.mx:1003/1822
Acceso en línea:http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1822
Access Level:acceso abierto
Palabra clave:info:eu-repo/classification/Autores/NUCLEOLUS
info:eu-repo/classification/Autores/RIBONUCLEOLAR PARTICLE
info:eu-repo/classification/Autores/RRNA
info:eu-repo/classification/Autores/FIBRILLARIN
info:eu-repo/classification/Autores/PHOSPHOINOSITIDES
info:eu-repo/classification/Autores/VIRAL PROGRESSION
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/24
info:eu-repo/classification/cti/2415
info:eu-repo/classification/cti/241502
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spelling Fibrillarin ribonuclease activity is dependent on the GAR domain and modulated by phospholipidsFrancisco Guillén-ChableULISES RODRIGUEZ CORONAAlejandro Pereira-SantanaANDREA BAYONA HERNANDEZLuis Carlos Rodríguez ZapataCecilia Aquino PerezLenka ŠebestováNicolas VitalePavel HozakEnrique Castaño de la Sernainfo:eu-repo/classification/Autores/NUCLEOLUSinfo:eu-repo/classification/Autores/RIBONUCLEOLAR PARTICLEinfo:eu-repo/classification/Autores/RRNAinfo:eu-repo/classification/Autores/FIBRILLARINinfo:eu-repo/classification/Autores/PHOSPHOINOSITIDESinfo:eu-repo/classification/Autores/VIRAL PROGRESSIONinfo:eu-repo/classification/cti/2info:eu-repo/classification/cti/24info:eu-repo/classification/cti/2415info:eu-repo/classification/cti/241502info:eu-repo/classification/cti/241502Fibrillarin is a highly conserved nucleolar methyltransferase responsible for ribosomal RNA methylation across evolution from Archaea to humans. It has been reported that fibrillarin is involved in the methylation of histone H2A in nucleoli and other processes, including viral progression, cellular stress, nuclear shape, and cell cycle progression. We show that fibrillarin has an additional activity as a ribonuclease. The activity is affected by phosphoinositides and phosphatidic acid and insensitive to ribonuclease inhibitors. Furthermore, the presence of phosphatidic acid releases the fibrillarin-U3 snoRNA complex. We show that the ribonuclease activity localizes to the GAR (glycine/arginine-rich) domain conserved in a small group of RNA interacting proteins. The introduction of the GAR domain occurred in evolution in the transition from archaea to eukaryotic cells. The interaction of this domain with phospholipids may allow a phase separation of this protein in nucleoli.2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://cicy.repositorioinstitucional.mx/jspui/handle/1003/1822Cells, 9(5), 1143. https://doi.org/10.3390/cells9051143reponame:Repositorio Institucional CICYinstname:Centro de Investigación Científica de Yucatáninstacron:CICYenginfo:eu-repo/semantics/datasetDOI/https://doi.org/10.3390/cells9051143citation:Guillen-Chable, F., Corona, U. R., Pereira-Santana, A., Bayona, A., Rodríguez-Zapata, L. C., Aquino, C., Šebestová, L., Vitale, N., Hozak, P., & Castano, E. (2020). Fibrillarin Ribonuclease Activity is Dependent on the GAR Domain and Modulated by Phospholipids. Cells, 9(5), 1143. https://doi.org/10.3390/cells9051143info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0oai:cicy.repositorioinstitucional.mx:1003/18222024-08-28T03:18:43Z
dc.title.none.fl_str_mv Fibrillarin ribonuclease activity is dependent on the GAR domain and modulated by phospholipids
title Fibrillarin ribonuclease activity is dependent on the GAR domain and modulated by phospholipids
spellingShingle Fibrillarin ribonuclease activity is dependent on the GAR domain and modulated by phospholipids
Francisco Guillén-Chable
info:eu-repo/classification/Autores/NUCLEOLUS
info:eu-repo/classification/Autores/RIBONUCLEOLAR PARTICLE
info:eu-repo/classification/Autores/RRNA
info:eu-repo/classification/Autores/FIBRILLARIN
info:eu-repo/classification/Autores/PHOSPHOINOSITIDES
info:eu-repo/classification/Autores/VIRAL PROGRESSION
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/24
info:eu-repo/classification/cti/2415
info:eu-repo/classification/cti/241502
info:eu-repo/classification/cti/241502
title_short Fibrillarin ribonuclease activity is dependent on the GAR domain and modulated by phospholipids
title_full Fibrillarin ribonuclease activity is dependent on the GAR domain and modulated by phospholipids
title_fullStr Fibrillarin ribonuclease activity is dependent on the GAR domain and modulated by phospholipids
title_full_unstemmed Fibrillarin ribonuclease activity is dependent on the GAR domain and modulated by phospholipids
title_sort Fibrillarin ribonuclease activity is dependent on the GAR domain and modulated by phospholipids
dc.creator.none.fl_str_mv Francisco Guillén-Chable
ULISES RODRIGUEZ CORONA
Alejandro Pereira-Santana
ANDREA BAYONA HERNANDEZ
Luis Carlos Rodríguez Zapata
Cecilia Aquino Perez
Lenka Šebestová
Nicolas Vitale
Pavel Hozak
Enrique Castaño de la Serna
author Francisco Guillén-Chable
author_facet Francisco Guillén-Chable
ULISES RODRIGUEZ CORONA
Alejandro Pereira-Santana
ANDREA BAYONA HERNANDEZ
Luis Carlos Rodríguez Zapata
Cecilia Aquino Perez
Lenka Šebestová
Nicolas Vitale
Pavel Hozak
Enrique Castaño de la Serna
author_role author
author2 ULISES RODRIGUEZ CORONA
Alejandro Pereira-Santana
ANDREA BAYONA HERNANDEZ
Luis Carlos Rodríguez Zapata
Cecilia Aquino Perez
Lenka Šebestová
Nicolas Vitale
Pavel Hozak
Enrique Castaño de la Serna
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv info:eu-repo/classification/Autores/NUCLEOLUS
info:eu-repo/classification/Autores/RIBONUCLEOLAR PARTICLE
info:eu-repo/classification/Autores/RRNA
info:eu-repo/classification/Autores/FIBRILLARIN
info:eu-repo/classification/Autores/PHOSPHOINOSITIDES
info:eu-repo/classification/Autores/VIRAL PROGRESSION
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/24
info:eu-repo/classification/cti/2415
info:eu-repo/classification/cti/241502
info:eu-repo/classification/cti/241502
topic info:eu-repo/classification/Autores/NUCLEOLUS
info:eu-repo/classification/Autores/RIBONUCLEOLAR PARTICLE
info:eu-repo/classification/Autores/RRNA
info:eu-repo/classification/Autores/FIBRILLARIN
info:eu-repo/classification/Autores/PHOSPHOINOSITIDES
info:eu-repo/classification/Autores/VIRAL PROGRESSION
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/24
info:eu-repo/classification/cti/2415
info:eu-repo/classification/cti/241502
info:eu-repo/classification/cti/241502
description Fibrillarin is a highly conserved nucleolar methyltransferase responsible for ribosomal RNA methylation across evolution from Archaea to humans. It has been reported that fibrillarin is involved in the methylation of histone H2A in nucleoli and other processes, including viral progression, cellular stress, nuclear shape, and cell cycle progression. We show that fibrillarin has an additional activity as a ribonuclease. The activity is affected by phosphoinositides and phosphatidic acid and insensitive to ribonuclease inhibitors. Furthermore, the presence of phosphatidic acid releases the fibrillarin-U3 snoRNA complex. We show that the ribonuclease activity localizes to the GAR (glycine/arginine-rich) domain conserved in a small group of RNA interacting proteins. The introduction of the GAR domain occurred in evolution in the transition from archaea to eukaryotic cells. The interaction of this domain with phospholipids may allow a phase separation of this protein in nucleoli.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1822
url http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1822
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/datasetDOI/https://doi.org/10.3390/cells9051143
citation:Guillen-Chable, F., Corona, U. R., Pereira-Santana, A., Bayona, A., Rodríguez-Zapata, L. C., Aquino, C., Šebestová, L., Vitale, N., Hozak, P., & Castano, E. (2020). Fibrillarin Ribonuclease Activity is Dependent on the GAR Domain and Modulated by Phospholipids. Cells, 9(5), 1143. https://doi.org/10.3390/cells9051143
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Cells, 9(5), 1143. https://doi.org/10.3390/cells9051143
reponame:Repositorio Institucional CICY
instname:Centro de Investigación Científica de Yucatán
instacron:CICY
instname_str Centro de Investigación Científica de Yucatán
instacron_str CICY
institution CICY
reponame_str Repositorio Institucional CICY
collection Repositorio Institucional CICY
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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