Evaluating the toxicity of nanomaterials in three-dimensional cellular models
Traditionally, in vitro evaluations to determine the cytotoxic effect of nanomaterials in cell cultures have been carried out in two-dimensional cultures, because the protocols to assess it have been adapted from those used in toxicology. However, the interactions between cells are much more complex...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2020 |
| País: | México |
| Institución: | UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO |
| Repositorio: | Mundo Nano. Revista Interdisciplinaria en Nanociencias y Nanotecnología |
| Idioma: | español |
| OAI Identifier: | oai:ojs.pkp.sfu.ca:article/69608 |
| Acceso en línea: | https://www.mundonano.unam.mx/ojs/index.php/nano/article/view/69608 |
| Access Level: | acceso abierto |
| Palabra clave: | spheroids nanotoxicology cytotoxicity cell culture esferoides nanotoxicología citotoxicidad cultivo celular |
| Sumario: | Traditionally, in vitro evaluations to determine the cytotoxic effect of nanomaterials in cell cultures have been carried out in two-dimensional cultures, because the protocols to assess it have been adapted from those used in toxicology. However, the interactions between cells are much more complex than those found in a monolayer arrangement. Thus, this is the main reason to promote the implementation of three-dimensional cell cultures also known as spheroids, to assess the effect of nanomaterials on cell cultures. Abundant supports the idea that spheroids represent a better model for the study of cellular responses, since they emulate more precisely the cellular junctions, communication and physiology that occurs in a tissue within an in vivo model. Herein, we discuss some points about the development of 3D cultures as a new and better methodology for nanotoxicological evaluations. |
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