Effect of graphene oxide on bacteria and peripheral blood mononuclear cells

"Background Driven by the potential biological applications of graphene, many groups have studied the response of cells exposed to graphene oxide (GO). In particular, investigations of bacteria indicate that there are 2 crucial parameters, which so far have only been investigated separately: GO...

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Autores: Jessica Rosaura Campos Delgado, José Gil Munguía López, Benjamin Fragneaud, Joyce Araujo, Francisco Gonzalez, HUGO RICARDO NAVARRO CONTRERAS, IVAN NELINHO PEREZ MALDONADO, Antonio de León Rodríguez
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:México
Institución:Instituto Potosino de Investigación Científica y Tecnológica
Repositorio:Repositorio Institucional del IPICYT
OAI Identifier:oai:ipicyt.repositorioinstitucional.mx:1010/1853
Acceso en línea:http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1853
Access Level:acceso abierto
Palabra clave:info:eu-repo/classification/Autor/Antibacterial effect
info:eu-repo/classification/Autor/Escherichia coli
info:eu-repo/classification/Autor/Graphene oxide
info:eu-repo/classification/Autor/Peripheral blood mononuclear cells
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/24
info:eu-repo/classification/cti/2406
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network_acronym_str MX
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repository_id_str
spelling Effect of graphene oxide on bacteria and peripheral blood mononuclear cellsJessica Rosaura Campos DelgadoJosé Gil Munguía LópezBenjamin FragneaudJoyce AraujoFrancisco GonzalezHUGO RICARDO NAVARRO CONTRERASIVAN NELINHO PEREZ MALDONADOAntonio de León Rodríguezinfo:eu-repo/classification/Autor/Antibacterial effectinfo:eu-repo/classification/Autor/Escherichia coliinfo:eu-repo/classification/Autor/Graphene oxideinfo:eu-repo/classification/Autor/Peripheral blood mononuclear cellsinfo:eu-repo/classification/cti/2info:eu-repo/classification/cti/24info:eu-repo/classification/cti/2406info:eu-repo/classification/cti/2406"Background Driven by the potential biological applications of graphene, many groups have studied the response of cells exposed to graphene oxide (GO). In particular, investigations of bacteria indicate that there are 2 crucial parameters, which so far have only been investigated separately: GO size and exposure methodology. Our study took into account both parameters. We carefully characterized the samples to catalog sizes and structural properties, and tested different exposure methodologies: exposure in saline solution and in the presence of growth media. Furthermore, we performed experiments with peripheral blood mononuclear cells exposed to our GO materials. Methods Atomic force microscopy, scanning electron microscopy, Raman spectroscopy, X-ray photoelectron spectroscopy and transmission electron microscopy were used to characterize the morphology and composition of different samples of GO: GO-H2O, GO-PBS and GO-MG. Our samples had 2D sizes of ?100 nm (GO-H2O and GO-PBS) and >2 µm (GO-MG). We tested antibacterial activity and cytotoxicity toward peripheral blood mononuclear cells of 3 different GO samples. Results A size-dependent growth inhibition of Escherichia coli (DH5 ?) in suspension was found, which proved that this effect depends strongly on the protocol followed for exposure. Hemocompatibility was confirmed by exposing peripheral blood mononuclear cells to materials for 24 hours; viability and apoptosis tests were also carried out. Conclusions Our experiments provide vital information for future applications of GO in suspension. If its antibacterial properties are to be potentiated, care should be taken to select 2D sizes in the micrometer range, and exposure should not be carried out in the presence of grow media."Wichtig Publishing2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1853reponame:Repositorio Institucional del IPICYTinstname:Instituto Potosino de Investigación Científica y Tecnológicainstacron:IPICYTinfo:eu-repo/semantics/altIdentifier/DOI/https://doi.org/10.5301/jabfm.5000325citation:Campos-Delgado, J., Castro, K. L. S., Munguia-Lopez, J. G., González, A. K., Mendoza, M. E., Fragneaud, B., … Achete, C. A. (2016). Effect of Graphene Oxide on Bacteria and Peripheral Blood Mononuclear Cells. Journal of Applied Biomaterials & Functional Materials, 14(4), 423–430.info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0oai:ipicyt.repositorioinstitucional.mx:1010/18532024-08-28T03:17:46Z
dc.title.none.fl_str_mv Effect of graphene oxide on bacteria and peripheral blood mononuclear cells
title Effect of graphene oxide on bacteria and peripheral blood mononuclear cells
spellingShingle Effect of graphene oxide on bacteria and peripheral blood mononuclear cells
Jessica Rosaura Campos Delgado
info:eu-repo/classification/Autor/Antibacterial effect
info:eu-repo/classification/Autor/Escherichia coli
info:eu-repo/classification/Autor/Graphene oxide
info:eu-repo/classification/Autor/Peripheral blood mononuclear cells
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/24
info:eu-repo/classification/cti/2406
info:eu-repo/classification/cti/2406
title_short Effect of graphene oxide on bacteria and peripheral blood mononuclear cells
title_full Effect of graphene oxide on bacteria and peripheral blood mononuclear cells
title_fullStr Effect of graphene oxide on bacteria and peripheral blood mononuclear cells
title_full_unstemmed Effect of graphene oxide on bacteria and peripheral blood mononuclear cells
title_sort Effect of graphene oxide on bacteria and peripheral blood mononuclear cells
dc.creator.none.fl_str_mv Jessica Rosaura Campos Delgado
José Gil Munguía López
Benjamin Fragneaud
Joyce Araujo
Francisco Gonzalez
HUGO RICARDO NAVARRO CONTRERAS
IVAN NELINHO PEREZ MALDONADO
Antonio de León Rodríguez
author Jessica Rosaura Campos Delgado
author_facet Jessica Rosaura Campos Delgado
José Gil Munguía López
Benjamin Fragneaud
Joyce Araujo
Francisco Gonzalez
HUGO RICARDO NAVARRO CONTRERAS
IVAN NELINHO PEREZ MALDONADO
Antonio de León Rodríguez
author_role author
author2 José Gil Munguía López
Benjamin Fragneaud
Joyce Araujo
Francisco Gonzalez
HUGO RICARDO NAVARRO CONTRERAS
IVAN NELINHO PEREZ MALDONADO
Antonio de León Rodríguez
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv info:eu-repo/classification/Autor/Antibacterial effect
info:eu-repo/classification/Autor/Escherichia coli
info:eu-repo/classification/Autor/Graphene oxide
info:eu-repo/classification/Autor/Peripheral blood mononuclear cells
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/24
info:eu-repo/classification/cti/2406
info:eu-repo/classification/cti/2406
topic info:eu-repo/classification/Autor/Antibacterial effect
info:eu-repo/classification/Autor/Escherichia coli
info:eu-repo/classification/Autor/Graphene oxide
info:eu-repo/classification/Autor/Peripheral blood mononuclear cells
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/24
info:eu-repo/classification/cti/2406
info:eu-repo/classification/cti/2406
description "Background Driven by the potential biological applications of graphene, many groups have studied the response of cells exposed to graphene oxide (GO). In particular, investigations of bacteria indicate that there are 2 crucial parameters, which so far have only been investigated separately: GO size and exposure methodology. Our study took into account both parameters. We carefully characterized the samples to catalog sizes and structural properties, and tested different exposure methodologies: exposure in saline solution and in the presence of growth media. Furthermore, we performed experiments with peripheral blood mononuclear cells exposed to our GO materials. Methods Atomic force microscopy, scanning electron microscopy, Raman spectroscopy, X-ray photoelectron spectroscopy and transmission electron microscopy were used to characterize the morphology and composition of different samples of GO: GO-H2O, GO-PBS and GO-MG. Our samples had 2D sizes of ?100 nm (GO-H2O and GO-PBS) and >2 µm (GO-MG). We tested antibacterial activity and cytotoxicity toward peripheral blood mononuclear cells of 3 different GO samples. Results A size-dependent growth inhibition of Escherichia coli (DH5 ?) in suspension was found, which proved that this effect depends strongly on the protocol followed for exposure. Hemocompatibility was confirmed by exposing peripheral blood mononuclear cells to materials for 24 hours; viability and apoptosis tests were also carried out. Conclusions Our experiments provide vital information for future applications of GO in suspension. If its antibacterial properties are to be potentiated, care should be taken to select 2D sizes in the micrometer range, and exposure should not be carried out in the presence of grow media."
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1853
url http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1853
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/DOI/https://doi.org/10.5301/jabfm.5000325
citation:Campos-Delgado, J., Castro, K. L. S., Munguia-Lopez, J. G., González, A. K., Mendoza, M. E., Fragneaud, B., … Achete, C. A. (2016). Effect of Graphene Oxide on Bacteria and Peripheral Blood Mononuclear Cells. Journal of Applied Biomaterials & Functional Materials, 14(4), 423–430.
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wichtig Publishing
publisher.none.fl_str_mv Wichtig Publishing
dc.source.none.fl_str_mv reponame:Repositorio Institucional del IPICYT
instname:Instituto Potosino de Investigación Científica y Tecnológica
instacron:IPICYT
instname_str Instituto Potosino de Investigación Científica y Tecnológica
instacron_str IPICYT
institution IPICYT
reponame_str Repositorio Institucional del IPICYT
collection Repositorio Institucional del IPICYT
repository.name.fl_str_mv
repository.mail.fl_str_mv
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