Identifcation of the mecA gene by PCR and PCR-RT of Staphylococcus aureus

At present, the application of more efficient techniques that contribute to the identification of pathogens such as Staphylococcus aureus more quickly has become essential because these pathogens are part of the main problem in nosocomial infections. The persistence of the problem is due to the fact...

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Autores: Palafox Rivera, Patricia, Palafox Félix, Michel, Peralta Mendoza, Jorge Alberto
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:México
Institución:UNIVERSIDAD DE SONORA
Repositorio:Epistemus
Idioma:español
OAI Identifier:oai:oai.epistemus.unison.mx:article/97
Acceso en línea:https://epistemus.unison.mx/index.php/epistemus/article/view/97
Access Level:acceso abierto
Palabra clave:Staphylococcus aureus
MRSA
PCR
PCR-RT
SARM
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spelling Identifcation of the mecA gene by PCR and PCR-RT of Staphylococcus aureusIDENTIFICACIÓN DEL GEN MECA POR PCR Y PCR-RT DE STAPHYLOCOCCUS AUREUS Palafox Rivera, PatriciaPalafox Félix, MichelPeralta Mendoza, Jorge AlbertoStaphylococcus aureusMRSAPCRPCR-RTStaphylococcus aureusSARMPCRPCR-RTAt present, the application of more efficient techniques that contribute to the identification of pathogens such as Staphylococcus aureus more quickly has become essential because these pathogens are part of the main problem in nosocomial infections. The persistence of the problem is due to the fact that these pathogens have developed resistance to environmental threats. The excessive use of sanitizers and antibiotics such as methicillin are the cause of the resistance that in thiscase comes from the mecA gene. The identification of this gene is crucial to differentiate resistant and non-resistant strains. However, taking into count that traditional processes for the identification of this pathogen are usually very slow, techniques such as PCR are applied because of their efficacy and efficiency. However, techniques such as PCR-RT have improvements that provide some advantage compared to its predecessor.En la actualidad, la aplicación de técnicas más eficientes que contribuyan a la identificación de patógenos como Staphylococcus aureus de forma más rápida se ha vuelto esencial debido a que estos patógenos forman parte de la principal problemática en infecciones nosocomiales. La persistencia del problema se debe a que estos patógenos han desarrollado resistencia ante las amenazas del medio ambiente. El uso excesivo de sanitizantes y antibióticos como la meticilina son los causantes de la resistencia que en este caso proviene del gen mecA. La identificación de este gen es determinante para diferenciar a las cepas resistentes y no resistentes. Sin embargo, tomando en cuenta que los procesos tradicionales para identificación de este patógeno suelen ser muy tardados, se aplican técnicas como el PCR, por su eficacia y eficiencia. No obstante, técnicas como el PCRRT poseen mejoras que aportan cierta ventaja en comparación con su predecesor.Universida de Sonora2019-06-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAcademyAcademiaapplication/pdftext/xmlhttps://epistemus.unison.mx/index.php/epistemus/article/view/9710.36790/epistemus.v13i26.97EPISTEMUS; Vol. 13 No. 26 (2019): Science does not stop; 55-58EPISTEMUS; Vol. 13 Núm. 26 (2019): La ciencia no cesa; 55-582007-81962007-4530reponame:Epistemusinstname:UNIVERSIDAD DE SONORAinstacron:UNISONspahttps://epistemus.unison.mx/index.php/epistemus/article/view/97/71https://epistemus.unison.mx/index.php/epistemus/article/view/97/155https://creativecommons.org/licenses/by-nc-nd/4.0info:eu-repo/semantics/openAccessoai:oai.epistemus.unison.mx:article/972024-08-19T22:27:29Z
dc.title.none.fl_str_mv Identifcation of the mecA gene by PCR and PCR-RT of Staphylococcus aureus
IDENTIFICACIÓN DEL GEN MECA POR PCR Y PCR-RT DE STAPHYLOCOCCUS AUREUS
title Identifcation of the mecA gene by PCR and PCR-RT of Staphylococcus aureus
spellingShingle Identifcation of the mecA gene by PCR and PCR-RT of Staphylococcus aureus
Palafox Rivera, Patricia
Staphylococcus aureus
MRSA
PCR
PCR-RT
Staphylococcus aureus
SARM
PCR
PCR-RT
title_short Identifcation of the mecA gene by PCR and PCR-RT of Staphylococcus aureus
title_full Identifcation of the mecA gene by PCR and PCR-RT of Staphylococcus aureus
title_fullStr Identifcation of the mecA gene by PCR and PCR-RT of Staphylococcus aureus
title_full_unstemmed Identifcation of the mecA gene by PCR and PCR-RT of Staphylococcus aureus
title_sort Identifcation of the mecA gene by PCR and PCR-RT of Staphylococcus aureus
dc.creator.none.fl_str_mv Palafox Rivera, Patricia
Palafox Félix, Michel
Peralta Mendoza, Jorge Alberto
author Palafox Rivera, Patricia
author_facet Palafox Rivera, Patricia
Palafox Félix, Michel
Peralta Mendoza, Jorge Alberto
author_role author
author2 Palafox Félix, Michel
Peralta Mendoza, Jorge Alberto
author2_role author
author
dc.subject.none.fl_str_mv Staphylococcus aureus
MRSA
PCR
PCR-RT
Staphylococcus aureus
SARM
PCR
PCR-RT
topic Staphylococcus aureus
MRSA
PCR
PCR-RT
Staphylococcus aureus
SARM
PCR
PCR-RT
description At present, the application of more efficient techniques that contribute to the identification of pathogens such as Staphylococcus aureus more quickly has become essential because these pathogens are part of the main problem in nosocomial infections. The persistence of the problem is due to the fact that these pathogens have developed resistance to environmental threats. The excessive use of sanitizers and antibiotics such as methicillin are the cause of the resistance that in thiscase comes from the mecA gene. The identification of this gene is crucial to differentiate resistant and non-resistant strains. However, taking into count that traditional processes for the identification of this pathogen are usually very slow, techniques such as PCR are applied because of their efficacy and efficiency. However, techniques such as PCR-RT have improvements that provide some advantage compared to its predecessor.
publishDate 2019
dc.date.none.fl_str_mv 2019-06-30
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Academy
Academia
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://epistemus.unison.mx/index.php/epistemus/article/view/97
10.36790/epistemus.v13i26.97
url https://epistemus.unison.mx/index.php/epistemus/article/view/97
identifier_str_mv 10.36790/epistemus.v13i26.97
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://epistemus.unison.mx/index.php/epistemus/article/view/97/71
https://epistemus.unison.mx/index.php/epistemus/article/view/97/155
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/xml
dc.publisher.none.fl_str_mv Universida de Sonora
publisher.none.fl_str_mv Universida de Sonora
dc.source.none.fl_str_mv EPISTEMUS; Vol. 13 No. 26 (2019): Science does not stop; 55-58
EPISTEMUS; Vol. 13 Núm. 26 (2019): La ciencia no cesa; 55-58
2007-8196
2007-4530
reponame:Epistemus
instname:UNIVERSIDAD DE SONORA
instacron:UNISON
instname_str UNIVERSIDAD DE SONORA
instacron_str UNISON
institution UNISON
reponame_str Epistemus
collection Epistemus
repository.name.fl_str_mv
repository.mail.fl_str_mv
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