Changes in the content of progesterone receptor isoforms and estrogen receptor alpha in the chick brain during embryonic development

Progesterone and estradiol participate in the regulation of several reproductive functions through interaction with intracellular progesterone receptors (PR) and estrogen receptors (ER), respectively. In this work, we determined PR and ER-alpha isoforms content in the brain of chicks of both sexes o...

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Detalles Bibliográficos
Autores: Camacho-Arroyo, I, Guerra-Araiza, C, González-Arenas, A, González-Aguero, G, González-Moran, G
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2003
País:México
Institución:Universidad Nacional Autónoma de México
Repositorio:Sistema de Información de la Facultad de Ciencias, UNAM
OAI Identifier:oai:repositorio.fciencias.unam.mx:11154/1684
Acceso en línea:http://hdl.handle.net/11154/1684
Access Level:acceso abierto
Palabra clave:Biochemistry & Molecular Biology
Physiology
Zoology
estradiol
progesterone
progesterone receptor isoforms
estrogen receptor alpha
chick brain
embryonic development
sexual dimorphism
sex steroid hormones
Descripción
Sumario:Progesterone and estradiol participate in the regulation of several reproductive functions through interaction with intracellular progesterone receptors (PR) and estrogen receptors (ER), respectively. In this work, we determined PR and ER-alpha isoforms content in the brain of chicks of both sexes on days 8 and 13 of embryonic development as well as on the day of hatching by Western blot analysis. PR isoforms protein content increased during embryonic development in both female and male chick brain. The highest PR isoforms content was observed on the day of hatching in both sexes. Interestingly, PR-A content was higher in the brain of chick males than in that of females on day 8 of embryonic development. PR-A/PR-B ratio was higher in the brain of males than in that of females at all ages. We found two ER-alpha isoforms of 66 and 52 kDa