GENETIC VARIABILITY OF PRSV-p ISOLATES FROM PAPAYO IN THE CENTRAL ZONE OF THE STATE OF VERACRUZ, MEXICO

Background: The Potyvirus Papaya ringspot virus type P (PRSV-p) causes the papaya (Carica papaya L.) ringspot disease, which causes damage to the plantations of this crop. In regions where there is movement of plant material and continuous production processes, there is greater genetic variability o...

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Autores: Sósol-Reyes, David, Villanueva-Jiménez, Juan A., Osorio-Acosta, Francisco, Flores-Estévez, Norma, Noa-Carrazana, Juan C.
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:México
Recursos:UNIVERSIDAD AUTÓNOMA DE YUCATÁN
Repositorio:Tropical and Subtropical Agroecosystems
Idioma:inglés
OAI Identifier:oai:ojs.www.revista.ccba.uady.mx:article/3887
Acesso em linha:https://www.revista.ccba.uady.mx/ojs/index.php/TSA/article/view/3887
Access Level:acceso abierto
Palavra-chave:Carica papaya; Papaya ringspot virus; sequence analysis; phylogeny; viral capsid.
análisis de secuencias; cápside viral; Carica papaya; filogenia; Papaya ringspot virus.
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network_acronym_str MX
network_name_str México
repository_id_str
dc.title.none.fl_str_mv GENETIC VARIABILITY OF PRSV-p ISOLATES FROM PAPAYO IN THE CENTRAL ZONE OF THE STATE OF VERACRUZ, MEXICO
VARIABILIDAD GENÉTICA DE AISLADOS DE PRSV-p DE PAPAYO EN LA ZONA CENTRO DEL ESTADO DE VERACRUZ, MÉXIC
title GENETIC VARIABILITY OF PRSV-p ISOLATES FROM PAPAYO IN THE CENTRAL ZONE OF THE STATE OF VERACRUZ, MEXICO
spellingShingle GENETIC VARIABILITY OF PRSV-p ISOLATES FROM PAPAYO IN THE CENTRAL ZONE OF THE STATE OF VERACRUZ, MEXICO
Sósol-Reyes, David
Carica papaya; Papaya ringspot virus; sequence analysis; phylogeny; viral capsid.
análisis de secuencias; cápside viral; Carica papaya; filogenia; Papaya ringspot virus.
title_short GENETIC VARIABILITY OF PRSV-p ISOLATES FROM PAPAYO IN THE CENTRAL ZONE OF THE STATE OF VERACRUZ, MEXICO
title_full GENETIC VARIABILITY OF PRSV-p ISOLATES FROM PAPAYO IN THE CENTRAL ZONE OF THE STATE OF VERACRUZ, MEXICO
title_fullStr GENETIC VARIABILITY OF PRSV-p ISOLATES FROM PAPAYO IN THE CENTRAL ZONE OF THE STATE OF VERACRUZ, MEXICO
title_full_unstemmed GENETIC VARIABILITY OF PRSV-p ISOLATES FROM PAPAYO IN THE CENTRAL ZONE OF THE STATE OF VERACRUZ, MEXICO
title_sort GENETIC VARIABILITY OF PRSV-p ISOLATES FROM PAPAYO IN THE CENTRAL ZONE OF THE STATE OF VERACRUZ, MEXICO
dc.creator.none.fl_str_mv Sósol-Reyes, David
Villanueva-Jiménez, Juan A.
Osorio-Acosta, Francisco
Flores-Estévez, Norma
Noa-Carrazana, Juan C.
author Sósol-Reyes, David
author_facet Sósol-Reyes, David
Villanueva-Jiménez, Juan A.
Osorio-Acosta, Francisco
Flores-Estévez, Norma
Noa-Carrazana, Juan C.
author_role author
author2 Villanueva-Jiménez, Juan A.
Osorio-Acosta, Francisco
Flores-Estévez, Norma
Noa-Carrazana, Juan C.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv
Consejo Nacional de Ciencia y Tecnología (CONACyT)
dc.subject.none.fl_str_mv Carica papaya; Papaya ringspot virus; sequence analysis; phylogeny; viral capsid.
análisis de secuencias; cápside viral; Carica papaya; filogenia; Papaya ringspot virus.
topic Carica papaya; Papaya ringspot virus; sequence analysis; phylogeny; viral capsid.
análisis de secuencias; cápside viral; Carica papaya; filogenia; Papaya ringspot virus.
description Background: The Potyvirus Papaya ringspot virus type P (PRSV-p) causes the papaya (Carica papaya L.) ringspot disease, which causes damage to the plantations of this crop. In regions where there is movement of plant material and continuous production processes, there is greater genetic variability of this virus; such is the case of the State of Veracruz, Mexico, one of the main papaya producers in the country. Objective: To analyze the genetic variability of isolates of PRSV-p, present in commercial papaya plantations in the central zone of the State of Veracruz. Methodology: Leaves with differential symptoms of the disease were collected. The capsid protein (CP) coding region was amplified by RT-PCR to confirm the identity of the virus. The sequences obtained were analyzed, aligned and a phylogenetic tree was obtained. Results: The presence of PRSV-p was confirmed by RT-PCR. Through sequence analysis, high diversity was observed in the first 50 amino acids encoded in the N-terminal region of CP. In the phylogenetic tree, three viral genetic groups were obtained. The largest conserved sites of interest (even the WCIEN box and QMKAAA) were found near the C-terminal region of the CP. 100% homology was observed in the central and C-terminal region of the Ver75Co isolate, with respect to the consensus sequence. Implications: The results show the genetic diversity of the virus in the central zone of the state of Veracruz, which will allow genetic modifications to improve resistance in papaya plants. Conclusions: There is genetic variability of PRSV-p in Veracruz, there are conserved sites of interest (WCIEN and QMKAAA) and changes in the N-terminal region, which includes the EK repetitive region and the DAG codon. These changes may be a genetic characteristic of the virus for adaptation. The Ver75Co isolate may be the point of change that gives rise to the other isolates of this work. The phylogenetic tree shows a single genetic group of the virus, which is distributed in three different clades.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.revista.ccba.uady.mx/ojs/index.php/TSA/article/view/3887
urn:ISSN:1870-0462-tsaes.v25i2.38870
10.56369/tsaes.3887
url https://www.revista.ccba.uady.mx/ojs/index.php/TSA/article/view/3887
identifier_str_mv urn:ISSN:1870-0462-tsaes.v25i2.38870
10.56369/tsaes.3887
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revista.ccba.uady.mx/ojs/index.php/TSA/article/view/3887/1819
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Autónoma de Yucatan
publisher.none.fl_str_mv Universidad Autónoma de Yucatan
dc.source.none.fl_str_mv Tropical and Subtropical Agroecosystems; Vol 25, No 2 (2022): (May - August)
Tropical and Subtropical Agroecosystems; Vol 25, No 2 (2022): (May - August)
1870-0462
reponame:Tropical and Subtropical Agroecosystems
instname:UNIVERSIDAD AUTÓNOMA DE YUCATÁN
instacron:UADY
instname_str UNIVERSIDAD AUTÓNOMA DE YUCATÁN
instacron_str UADY
institution UADY
reponame_str Tropical and Subtropical Agroecosystems
collection Tropical and Subtropical Agroecosystems
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling GENETIC VARIABILITY OF PRSV-p ISOLATES FROM PAPAYO IN THE CENTRAL ZONE OF THE STATE OF VERACRUZ, MEXICOVARIABILIDAD GENÉTICA DE AISLADOS DE PRSV-p DE PAPAYO EN LA ZONA CENTRO DEL ESTADO DE VERACRUZ, MÉXICSósol-Reyes, DavidVillanueva-Jiménez, Juan A.Osorio-Acosta, FranciscoFlores-Estévez, NormaNoa-Carrazana, Juan C.Carica papaya; Papaya ringspot virus; sequence analysis; phylogeny; viral capsid.análisis de secuencias; cápside viral; Carica papaya; filogenia; Papaya ringspot virus.Background: The Potyvirus Papaya ringspot virus type P (PRSV-p) causes the papaya (Carica papaya L.) ringspot disease, which causes damage to the plantations of this crop. In regions where there is movement of plant material and continuous production processes, there is greater genetic variability of this virus; such is the case of the State of Veracruz, Mexico, one of the main papaya producers in the country. Objective: To analyze the genetic variability of isolates of PRSV-p, present in commercial papaya plantations in the central zone of the State of Veracruz. Methodology: Leaves with differential symptoms of the disease were collected. The capsid protein (CP) coding region was amplified by RT-PCR to confirm the identity of the virus. The sequences obtained were analyzed, aligned and a phylogenetic tree was obtained. Results: The presence of PRSV-p was confirmed by RT-PCR. Through sequence analysis, high diversity was observed in the first 50 amino acids encoded in the N-terminal region of CP. In the phylogenetic tree, three viral genetic groups were obtained. The largest conserved sites of interest (even the WCIEN box and QMKAAA) were found near the C-terminal region of the CP. 100% homology was observed in the central and C-terminal region of the Ver75Co isolate, with respect to the consensus sequence. Implications: The results show the genetic diversity of the virus in the central zone of the state of Veracruz, which will allow genetic modifications to improve resistance in papaya plants. Conclusions: There is genetic variability of PRSV-p in Veracruz, there are conserved sites of interest (WCIEN and QMKAAA) and changes in the N-terminal region, which includes the EK repetitive region and the DAG codon. These changes may be a genetic characteristic of the virus for adaptation. The Ver75Co isolate may be the point of change that gives rise to the other isolates of this work. The phylogenetic tree shows a single genetic group of the virus, which is distributed in three different clades.Antecedentes: El Potyvirus Papaya ringspot virus tipo P (PRSV-p) ocasiona la enfermedad de la mancha anular del papayo (Carica papaya L.) la cual provoca daños en las plantaciones de este cultivo. En regiones donde hay movimiento de material vegetal y procesos de producción continua, existe mayor variabilidad genética de este virus; tal es el caso del estado de Veracruz, México, uno de los principales productores de papaya en el país. Objetivo: Analizar la variabilidad genética de aislados de PRSV-p, presente en plantaciones comerciales de papayo en la zona centro del estado de Veracruz. Metodología: Se realizaron colectas de hojas con síntomas diferenciales de la enfermedad. Se amplificó la región que codifica la proteína de la cápside (CP) mediante RT-PCR para confirmar la identidad del virus. Las secuencias obtenidas se analizaron, se alinearon y se obtuvo un árbol filogenético. Resultados: Se confirmó la presencia de PRSV-p a través de RT-PCR. Mediante el análisis de secuencias, se observó alta diversidad en los primeros 50 aminoácidos codificados en la región N-terminal de la CP. Se obtuvieron tres grupos genéticos virales en el árbol filogenético. Se encontraron sitios conservados de interés (incluida la caja WCIEN y QMKAAA), los de mayor tamaño, cerca de la región C-terminal de la CP. Se observó 100% de homología en la región central y C-terminal del aislado Ver75Co, respecto a la secuencia consenso. Implicaciones: Los resultados muestran la diversidad genética del virus en la zona central del estado de Veracruz, los cuales permitirán realizar modificaciones genéticas para mejorar la resistencia en plantas de papaya. Conclusión: Existe variabilidad genética de PRSV-p en Veracruz, se encuentran sitios conservados de interés (WCIEN y QMKAAA) y cambios en la región N-terminal, lo que incluye la región repetitiva EK y el codón DAG. Estos cambios pueden ser una característica genética del virus para la adaptación. El aislado Ver75Co, puede ser el punto de cambio que da origen a los otros aislados de este trabajo. El árbol filogenético muestra un solo grupo genético del virus, el cual está distribuido en tres clados distintos.Universidad Autónoma de YucatanConsejo Nacional de Ciencia y Tecnología (CONACyT)2022-04-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revista.ccba.uady.mx/ojs/index.php/TSA/article/view/3887urn:ISSN:1870-0462-tsaes.v25i2.3887010.56369/tsaes.3887Tropical and Subtropical Agroecosystems; Vol 25, No 2 (2022): (May - August)Tropical and Subtropical Agroecosystems; Vol 25, No 2 (2022): (May - August)1870-0462reponame:Tropical and Subtropical Agroecosystemsinstname:UNIVERSIDAD AUTÓNOMA DE YUCATÁNinstacron:UADYenghttps://www.revista.ccba.uady.mx/ojs/index.php/TSA/article/view/3887/1819Copyright (c) 2022 David Sósol-Reyes, Juan Antonio Villanueva-Jiménez, Francisco Osorio-Acosta, Norma Flores-Estévez, Juan Carlos Noa-Carrazanahttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessoai:ojs.www.revista.ccba.uady.mx:article/38872024-08-22T17:23:06Z
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