Monocillium gamsii sp. nov. and Monocillium bulbillosum: two nematode-associated fungi parasitising the eggs of Heterodera filipjevi

Monocillium gamsii sp. nov. (Ascomycota, Hypocreales, Niessliaceae) isolated from eggs of the cereal cyst nematode Heterodera filipjevi is described and illustrated based on morphological and molecular phylogenetic evidence. The new taxon discovered in wheat fields in Turkey destructively parasitise...

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Detalles Bibliográficos
Autores: Ashrafi, S., Stadler, M., Dababat, A.A., Richert-Pöggeler, K.R., Finckh, M., Maier, W.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:México
Institución:Centro Internacional de Mejoramiento de Maíz y Trigo
Repositorio:Repositorio Institucional de Publicaciones Multimedia del CIMMYT
OAI Identifier:oai:repository.cimmyt.org:10883/19164
Acceso en línea:http://hdl.handle.net/10883/19164
Access Level:acceso abierto
Palabra clave:AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
Egg-Parasitic Fungi
Niessliaceae
New Species
Plant Parasitic Nematodes
Molecular Phylogeny
ITS
LSU
Rpb1
Translation-Elongation Factor
FUNGI
PARASITISM
NEMATODA
EGGS
PLANT NEMATODES
MOLECULAR BIOLOGY
PHYLOGENY
Descripción
Sumario:Monocillium gamsii sp. nov. (Ascomycota, Hypocreales, Niessliaceae) isolated from eggs of the cereal cyst nematode Heterodera filipjevi is described and illustrated based on morphological and molecular phylogenetic evidence. The new taxon discovered in wheat fields in Turkey destructively parasitises nematode eggs. The infected eggs were readily colonised by the fungus, which produced microsclerotia. The fungus could be grown on artificial media and the parasitism of M. gamsii towards H. filipjevi was reproducible in vitro. Hyphae penetrating the nematode eggs entirely colonised the embryo, developed into multicellular chlamydospore and dictyochlamydospore-like structures eventually forming microsclerotia. Molecular and morphological differences and similarities between M. gamsii and its phylogenetically related species are discussed. Monocillium bulbillosum was found to be closely related to the new species. The pathogenicity of M. bulbillosum against H. filipjevi was also assayed in vitro because of its sister group relationship to M. gamsii revealing that this species was also capable of colonising eggs of H. filipjevi.