Antibacterial activity in the hemolymph of the catarina scallop Argopecten ventricosus
We conducted a search for antibacterial peptide like activityin hemolymph of Argopecten ventricosus. Pre-purification of peptideswas done by reverse phase HPLC. Hemolymph acidic supernatantwas loaded into a column packed with C18 matrix. Stepwise elutionswere performed with 5, 50, 80, and 100 % acet...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2007 |
| País: | México |
| Institución: | Centro de Investigaciones Biológicas del Noroeste, S.C. |
| Repositorio: | Redalyc-CIBNOR |
| OAI Identifier: | oai:redalyc.org:57811711 |
| Acceso en línea: | https://www.redalyc.org/articulo.oa?id=57811711 |
| Access Level: | acceso abierto |
| Palabra clave: | Biología immunity hemolymph Vibrio alginolyticus Antimicrobial peptide Argopecten ventricosus |
| Sumario: | We conducted a search for antibacterial peptide like activityin hemolymph of Argopecten ventricosus. Pre-purification of peptideswas done by reverse phase HPLC. Hemolymph acidic supernatantwas loaded into a column packed with C18 matrix. Stepwise elutionswere performed with 5, 50, 80, and 100 % acetonitrile (ACN) in0.05 % trifluoroacetic acid (TFA) over 550 min at a flow rate of 0.2ml/min. Absorbance was monitored at 280 nm. Eluted fractions wereconcentrated under vacuum. Vibrio alginolyticus 138-2 was used as amodel to test fractions. Growth inhibition zone were observed at 5 and50 % ACN. This is the first report of antimicrobial peptide-like activityin hemolymph of a pectinid species. |
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