Development and validation of red root marker-based haploid inducers that effectively complement R1-nj (Navajo) marker-based in vivo haploid identification in maize

One of the critical limitations for the in vivo production of doubled haploid (DH) lines in maize (Zea mays L.) is the inability to effectively identify haploids in a significant proportion of induction crosses due to the possibility of complete or partial inhibition of the currently used R1‐nj (Nav...

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Autores: Chaikam, V., Martinez, L., Melchinger, A.E., Schipprack, W., Prasanna, B.M.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:México
Institución:Centro Internacional de Mejoramiento de Maíz y Trigo
Repositorio:Repositorio Institucional de Publicaciones Multimedia del CIMMYT
OAI Identifier:oai:repository.cimmyt.org:10883/17041
Acceso en línea:http://hdl.handle.net/10883/17041
Access Level:acceso abierto
Palabra clave:AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
HAPLOIDY
MAIZE
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spelling Development and validation of red root marker-based haploid inducers that effectively complement R1-nj (Navajo) marker-based in vivo haploid identification in maizeChaikam, V.Martinez, L.Melchinger, A.E.Schipprack, W.Prasanna, B.M.AGRICULTURAL SCIENCES AND BIOTECHNOLOGYHAPLOIDYMAIZEOne of the critical limitations for the in vivo production of doubled haploid (DH) lines in maize (Zea mays L.) is the inability to effectively identify haploids in a significant proportion of induction crosses due to the possibility of complete or partial inhibition of the currently used R1‐nj (Navajo) color marker. In this study, we demonstrate that the R1‐nj marker could result in a high proportion of false positives among the haploids identified, besides being ineffective in germplasm with natural anthocyanin expression in pericarp tissue. To address these limitations, we developed haploid inducer lines with triple anthocyanin color markers, including the expression of anthocyanin coloration in the seedling roots and leaf sheaths, in addition to the Navajo marker on the seed. Although these inducers show acceptable haploid induction rates ranging from 8.6 to 10.2%, they exhibited relatively poor agronomic performance compared with tropicalized haploid inducers within tropical environments. The addition of the red root marker more accurately identified haploids among the germinating seedlings, including four tropical inbred lines and eight breeding populations that showed complete inhibition of R1‐nj. We also demonstrate that the red root marker can be used for haploid identification in germplasm with natural anthocyanin expression in the pericarp. A survey of 546 tropical inbreds and 244 landraces showed that anthocyanin accumulation in the roots of germinating seedlings is very rare compared with anthocyanin accumulation in the seed and leaf sheath tissues. As a result, the red root marker can serve as a highly complementary marker to R1‐nj to enable effective identification of haploids within a wide range of tropical maize germplasm.1678-1688Crop Science Society of America (CSSA)2016-07-05T14:41:23Z2016-07-05T14:41:23Z2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePDFapplication/pdfhttp://hdl.handle.net/10883/1704110.2135/cropsci2015.10.0653456Crop Sciencereponame:Repositorio Institucional de Publicaciones Multimedia del CIMMYTinstname:Centro Internacional de Mejoramiento de Maíz y Trigoinstacron:CIMMYTEnglishhttps://acsess.onlinelibrary.wiley.com/doi/full/10.2135/cropsci2015.10.0653#support-information-sectionUSACIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose.Open Accessinfo:eu-repo/semantics/openAccessoai:repository.cimmyt.org:10883/170412024-10-11T19:55:21Z
dc.title.none.fl_str_mv Development and validation of red root marker-based haploid inducers that effectively complement R1-nj (Navajo) marker-based in vivo haploid identification in maize
title Development and validation of red root marker-based haploid inducers that effectively complement R1-nj (Navajo) marker-based in vivo haploid identification in maize
spellingShingle Development and validation of red root marker-based haploid inducers that effectively complement R1-nj (Navajo) marker-based in vivo haploid identification in maize
Chaikam, V.
AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
HAPLOIDY
MAIZE
title_short Development and validation of red root marker-based haploid inducers that effectively complement R1-nj (Navajo) marker-based in vivo haploid identification in maize
title_full Development and validation of red root marker-based haploid inducers that effectively complement R1-nj (Navajo) marker-based in vivo haploid identification in maize
title_fullStr Development and validation of red root marker-based haploid inducers that effectively complement R1-nj (Navajo) marker-based in vivo haploid identification in maize
title_full_unstemmed Development and validation of red root marker-based haploid inducers that effectively complement R1-nj (Navajo) marker-based in vivo haploid identification in maize
title_sort Development and validation of red root marker-based haploid inducers that effectively complement R1-nj (Navajo) marker-based in vivo haploid identification in maize
dc.creator.none.fl_str_mv Chaikam, V.
Martinez, L.
Melchinger, A.E.
Schipprack, W.
Prasanna, B.M.
author Chaikam, V.
author_facet Chaikam, V.
Martinez, L.
Melchinger, A.E.
Schipprack, W.
Prasanna, B.M.
author_role author
author2 Martinez, L.
Melchinger, A.E.
Schipprack, W.
Prasanna, B.M.
author2_role author
author
author
author
dc.subject.none.fl_str_mv AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
HAPLOIDY
MAIZE
topic AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
HAPLOIDY
MAIZE
description One of the critical limitations for the in vivo production of doubled haploid (DH) lines in maize (Zea mays L.) is the inability to effectively identify haploids in a significant proportion of induction crosses due to the possibility of complete or partial inhibition of the currently used R1‐nj (Navajo) color marker. In this study, we demonstrate that the R1‐nj marker could result in a high proportion of false positives among the haploids identified, besides being ineffective in germplasm with natural anthocyanin expression in pericarp tissue. To address these limitations, we developed haploid inducer lines with triple anthocyanin color markers, including the expression of anthocyanin coloration in the seedling roots and leaf sheaths, in addition to the Navajo marker on the seed. Although these inducers show acceptable haploid induction rates ranging from 8.6 to 10.2%, they exhibited relatively poor agronomic performance compared with tropicalized haploid inducers within tropical environments. The addition of the red root marker more accurately identified haploids among the germinating seedlings, including four tropical inbred lines and eight breeding populations that showed complete inhibition of R1‐nj. We also demonstrate that the red root marker can be used for haploid identification in germplasm with natural anthocyanin expression in the pericarp. A survey of 546 tropical inbreds and 244 landraces showed that anthocyanin accumulation in the roots of germinating seedlings is very rare compared with anthocyanin accumulation in the seed and leaf sheath tissues. As a result, the red root marker can serve as a highly complementary marker to R1‐nj to enable effective identification of haploids within a wide range of tropical maize germplasm.
publishDate 2016
dc.date.none.fl_str_mv 2016-07-05T14:41:23Z
2016-07-05T14:41:23Z
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10883/17041
10.2135/cropsci2015.10.0653
url http://hdl.handle.net/10883/17041
identifier_str_mv 10.2135/cropsci2015.10.0653
dc.language.none.fl_str_mv English
language_invalid_str_mv English
dc.relation.none.fl_str_mv https://acsess.onlinelibrary.wiley.com/doi/full/10.2135/cropsci2015.10.0653#support-information-section
dc.rights.none.fl_str_mv Open Access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Open Access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv PDF
application/pdf
dc.coverage.none.fl_str_mv USA
dc.publisher.none.fl_str_mv Crop Science Society of America (CSSA)
publisher.none.fl_str_mv Crop Science Society of America (CSSA)
dc.source.none.fl_str_mv 4
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Crop Science
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