Implementing Stepwise Solvent Elution in Multisyringe Liquid Chromatography (MSC) to Separate Norepinephrine, Epinephrine and Dopamine
A multisyringe liquid chromatographic (MSC) method by using stepwise solvent elution and reverse-phase liquid chromatography C18 monolithic column to separate Epinephrine (E), Norepinephrine (NE) and Dopamine (DA) was developed. The first step employs the mixture sodium1-hexanosulfonate monohydrate...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2012 |
| País: | México |
| Institución: | Universidad Nacional Autónoma de México |
| Repositorio: | Redalyc-UNAM |
| OAI Identifier: | oai:redalyc.org:47523306019 |
| Acceso en línea: | https://www.redalyc.org/articulo.oa?id=47523306019 |
| Access Level: | acceso abierto |
| Palabra clave: | Química Catecholamines Stepwise elution C18 monolithic column Multisyringe liquid chromatography |
| Sumario: | A multisyringe liquid chromatographic (MSC) method by using stepwise solvent elution and reverse-phase liquid chromatography C18 monolithic column to separate Epinephrine (E), Norepinephrine (NE) and Dopamine (DA) was developed. The first step employs the mixture sodium1-hexanosulfonate monohydrate (HS) 7.5mM: methanol (98:2) to separate NE and E and the second step uses HS (5. mM): methanol (92:8) to elute DA. The three analytes were separated on 6 min spending only 10 mL of mobile phases. The detection limits were between 1.3 ug mL-1 (DA) and 2.04 ug mL-1 (NE). |
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