Isolation and Identification of Herpesvirus in the Bottlenose Dolphins (Tursiops truncatus) of Terminos Lagoon, Campeche, Mexico

ntroduction: Alphaherpesviruses have been associated with fatal systemic infections in several cetartiodactyla species. The main goal in this paper is to identify microorganisms in wild bottlenose dolphin samples were taken from animals in Campeche, Mexico. Methods: Eight free-living bottlenose dolp...

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Autores: E. G. Valdivia-Lara, A. Delgado-Estrella, J. I. Ángeles-Solís, E. N. Ortuño de la O, S. González-Gallardo, G .E. Lara-Reyes, C. Cuenca-Verde, G. Valdivia-Anda
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:México
Recursos:Universidad Autónoma del Carmen
Repositorio:Redalyc-UNACAR
OAI Identifier:oai:redalyc.org:402339248017
Acesso em linha:https://www.redalyc.org/articulo.oa?id=402339248017
https://www.redalyc.org/journal/4023/402339248017/
https://www.redalyc.org/journal/4023/402339248017/html/
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Access Level:acceso abierto
Palavra-chave:Biología
Mexico
Herpesvirus
wild dolphins
Terminos lagoon
Bottlenose dolphins
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spelling Isolation and Identification of Herpesvirus in the Bottlenose Dolphins (Tursiops truncatus) of Terminos Lagoon, Campeche, MexicoE. G. Valdivia-LaraA. Delgado-EstrellaJ. I. Ángeles-SolísE. N. Ortuño de la OS. González-GallardoG .E. Lara-ReyesC. Cuenca-VerdeG. Valdivia-AndaBiologíaMexicoHerpesviruswild dolphinsTerminos lagoonBottlenose dolphinsntroduction: Alphaherpesviruses have been associated with fatal systemic infections in several cetartiodactyla species. The main goal in this paper is to identify microorganisms in wild bottlenose dolphin samples were taken from animals in Campeche, Mexico. Methods: Eight free-living bottlenose dolphins (Tursiops truncatus) from the Terminos Lagoon, Mexico, were captured, sampled and released. The animals were sampled for their blood, blow hole secretion, vaginal or prepuce discharge and skin. From the exudates, the cytology was examined, and cell inoculation was performed using bovine kidney cells (MDBK), African green monkey kidney cells (VERO), canine kidney cells (MDCK) and porcine kidney cells (PK15). Results: After observing the cytopathic effects, the isolates were replicated in the same cell line at least three times. Three isolates were obtained that had a cytolytic effect at 48 – 72 hr. A previously described nested PCR targeting highly conserved regions of the herpesvirus DNA polymerase was performed, as well as transmission electron microscopy (TEM) and immunofluorescence on the infected MDBK cells. Discussion and conclusions: The animals from which the isolates were obtained were clinically healthy at the time of their capture, and it is likely that these animals act as carriers or reservoirs of the virus.Asociación Mexicana de Mastozoología2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdf2007-3364https://www.redalyc.org/articulo.oa?id=402339248017https://www.redalyc.org/journal/4023/402339248017/https://www.redalyc.org/journal/4023/402339248017/html/https://www.redalyc.org/journal/4023/402339248017/402339248017.epubhttps://www.redalyc.org/journal/4023/402339248017/movilTherya (México) Num.2 Vol.6reponame:Redalyc-UNACARinstname:Universidad Autónoma del Carmeninstacron:UNACARenhttp://www.redalyc.org/revista.oa?id=4023Theryainfo:eu-repo/semantics/openAccessoai:redalyc.org:4023392480172024-08-23T21:47:53Z
dc.title.none.fl_str_mv Isolation and Identification of Herpesvirus in the Bottlenose Dolphins (Tursiops truncatus) of Terminos Lagoon, Campeche, Mexico
title Isolation and Identification of Herpesvirus in the Bottlenose Dolphins (Tursiops truncatus) of Terminos Lagoon, Campeche, Mexico
spellingShingle Isolation and Identification of Herpesvirus in the Bottlenose Dolphins (Tursiops truncatus) of Terminos Lagoon, Campeche, Mexico
E. G. Valdivia-Lara
Biología
Mexico
Herpesvirus
wild dolphins
Terminos lagoon
Bottlenose dolphins
title_short Isolation and Identification of Herpesvirus in the Bottlenose Dolphins (Tursiops truncatus) of Terminos Lagoon, Campeche, Mexico
title_full Isolation and Identification of Herpesvirus in the Bottlenose Dolphins (Tursiops truncatus) of Terminos Lagoon, Campeche, Mexico
title_fullStr Isolation and Identification of Herpesvirus in the Bottlenose Dolphins (Tursiops truncatus) of Terminos Lagoon, Campeche, Mexico
title_full_unstemmed Isolation and Identification of Herpesvirus in the Bottlenose Dolphins (Tursiops truncatus) of Terminos Lagoon, Campeche, Mexico
title_sort Isolation and Identification of Herpesvirus in the Bottlenose Dolphins (Tursiops truncatus) of Terminos Lagoon, Campeche, Mexico
dc.creator.none.fl_str_mv E. G. Valdivia-Lara
A. Delgado-Estrella
J. I. Ángeles-Solís
E. N. Ortuño de la O
S. González-Gallardo
G .E. Lara-Reyes
C. Cuenca-Verde
G. Valdivia-Anda
author E. G. Valdivia-Lara
author_facet E. G. Valdivia-Lara
A. Delgado-Estrella
J. I. Ángeles-Solís
E. N. Ortuño de la O
S. González-Gallardo
G .E. Lara-Reyes
C. Cuenca-Verde
G. Valdivia-Anda
author_role author
author2 A. Delgado-Estrella
J. I. Ángeles-Solís
E. N. Ortuño de la O
S. González-Gallardo
G .E. Lara-Reyes
C. Cuenca-Verde
G. Valdivia-Anda
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biología
Mexico
Herpesvirus
wild dolphins
Terminos lagoon
Bottlenose dolphins
topic Biología
Mexico
Herpesvirus
wild dolphins
Terminos lagoon
Bottlenose dolphins
description ntroduction: Alphaherpesviruses have been associated with fatal systemic infections in several cetartiodactyla species. The main goal in this paper is to identify microorganisms in wild bottlenose dolphin samples were taken from animals in Campeche, Mexico. Methods: Eight free-living bottlenose dolphins (Tursiops truncatus) from the Terminos Lagoon, Mexico, were captured, sampled and released. The animals were sampled for their blood, blow hole secretion, vaginal or prepuce discharge and skin. From the exudates, the cytology was examined, and cell inoculation was performed using bovine kidney cells (MDBK), African green monkey kidney cells (VERO), canine kidney cells (MDCK) and porcine kidney cells (PK15). Results: After observing the cytopathic effects, the isolates were replicated in the same cell line at least three times. Three isolates were obtained that had a cytolytic effect at 48 – 72 hr. A previously described nested PCR targeting highly conserved regions of the herpesvirus DNA polymerase was performed, as well as transmission electron microscopy (TEM) and immunofluorescence on the infected MDBK cells. Discussion and conclusions: The animals from which the isolates were obtained were clinically healthy at the time of their capture, and it is likely that these animals act as carriers or reservoirs of the virus.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 2007-3364
https://www.redalyc.org/articulo.oa?id=402339248017
https://www.redalyc.org/journal/4023/402339248017/
https://www.redalyc.org/journal/4023/402339248017/html/
https://www.redalyc.org/journal/4023/402339248017/402339248017.epub
https://www.redalyc.org/journal/4023/402339248017/movil
identifier_str_mv 2007-3364
url https://www.redalyc.org/articulo.oa?id=402339248017
https://www.redalyc.org/journal/4023/402339248017/
https://www.redalyc.org/journal/4023/402339248017/html/
https://www.redalyc.org/journal/4023/402339248017/402339248017.epub
https://www.redalyc.org/journal/4023/402339248017/movil
dc.language.none.fl_str_mv en
language_invalid_str_mv en
dc.relation.none.fl_str_mv http://www.redalyc.org/revista.oa?id=4023
dc.rights.none.fl_str_mv Therya
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Therya
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Asociación Mexicana de Mastozoología
publisher.none.fl_str_mv Asociación Mexicana de Mastozoología
dc.source.none.fl_str_mv Therya (México) Num.2 Vol.6
reponame:Redalyc-UNACAR
instname:Universidad Autónoma del Carmen
instacron:UNACAR
instname_str Universidad Autónoma del Carmen
instacron_str UNACAR
institution UNACAR
reponame_str Redalyc-UNACAR
collection Redalyc-UNACAR
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 14,965132

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