Análisis del Efecto de la Inactivación Fotodinámica con Diferentes Fotosensibilizadores en la Inhibición de Sporothrix schenckii spp
Sporotrichosis is a subcutaneous infection caused by species of the complex Sporothrix schenckii, dimorphic fungi of worldwide distribution. In the Mexican Republic it is the most frequent subcutaneous mycosis and is related to the occupation of the population. Due to the limitations and long period...
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| Tipo de recurso: | tesis de maestría |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2021 |
| País: | México |
| Institución: | Instituto Nacional de Astrofísica, Óptica y Electrónica |
| Repositorio: | Repositorio Institucional del INAOE |
| Idioma: | español |
| OAI Identifier: | oai:inaoe.repositorioinstitucional.mx:1009/2168 |
| Acceso en línea: | http://inaoe.repositorioinstitucional.mx/jspui/handle/1009/2168 |
| Access Level: | acceso abierto |
| Palabra clave: | info:eu-repo/classification/Esporotricosis/Sporotrichosis info:eu-repo/classification/Sporothrix schenckii/Sporothrix schenckii info:eu-repo/classification/Fotosensibilizador/Photosensitizer info:eu-repo/classification/Inactivación fotodinámica/Photodynamic inactivation info:eu-repo/classification/cti/7 info:eu-repo/classification/cti/33 info:eu-repo/classification/cti/3314 info:eu-repo/classification/cti/331110 |
| Sumario: | Sporotrichosis is a subcutaneous infection caused by species of the complex Sporothrix schenckii, dimorphic fungi of worldwide distribution. In the Mexican Republic it is the most frequent subcutaneous mycosis and is related to the occupation of the population. Due to the limitations and long periods of conventional mycosis treatments, it leads us to the search for a new therapeutic alternative. To do this, our study is based on identifying antifungal sensitivity and analyzing the effect of photodynamic inactivation (IFD) with different photosensitizers (FS) in S. schenckii yeast from different sources (patient, pet and nature). In the study, either 50 µg/mL of ketoconazole or 25 µg/mL of fluconazole were used as antifungal drugs and the method of disc diffusion was followed. As for the IFD, either 5 µM of Bengal Rose (RB) and 20 µM Curcumin were used as FS and light-emitting diodes (LED) as light sources with wavelengths of 520 nm and 460 nm; 4 control groups were proposed; non-irradiated group without FS (L-F-), group only with FS (L-F+), irradiated group with and without FS (L+F+, L+F-). As a result, all S. schenckii strains were resistant to ketoconazole and fluconazole. IFD significantly reduced S. schenckii using RB at 10 J/cm² and completely inhibited the fungus with curcumin at 20 J/cm², each with respect to the LF- control group. In conclusion, antifungal drugs are not effective in eliminating S. schenckii at the concentrations used in this study, while IFD with RB or curcumin in combination with LED are effective in inhibiting S. schenckii yeasts in in-vitro studies. |
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