The 16S rRNA gene in the study of marine microbial communities

New sequencing technologies a nd analytical capabilities have stimulated the study of micr obial communities from specific environments, enabling researchers to underst and the complexity of those systems. The 16S rRNA gene has proved very useful in d escribing the diversity and characte rization of...

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Detalles Bibliográficos
Autores: Fabiola Valenzuela-González, Ramón Casillas-Hernández, Enrique Villalpando, Francisco Vargas-Albores
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:México
Institución:Centro de Investigación en Alimentación y Desarrollo, A.C.
Repositorio:Redalyc-CIAD
OAI Identifier:oai:redalyc.org:48043157004
Acceso en línea:https://www.redalyc.org/articulo.oa?id=48043157004
Access Level:acceso abierto
Palabra clave:Ciencias de la Tierra
Metagenomics
16S rRNA databases
marine microbial communities
Descripción
Sumario:New sequencing technologies a nd analytical capabilities have stimulated the study of micr obial communities from specific environments, enabling researchers to underst and the complexity of those systems. The 16S rRNA gene has proved very useful in d escribing the diversity and characte rization of marine microbi al communities, particularly of unculti vated organisms. The development of new sequencing techniques has contributed to the exponential increase in the number of re ported 16S rRNA sequences as barcodes for microorganisms, forcing a review of concep ts and methods for the taxonom ic classification of these organisms. Ma nipulation and analysis of large amounts of genetic informati on have prompted the development of specific da tabases, specia lized algorithms, and computati onal tools to compare thousands of such sequences and make a taxonomic assignment. Comp lete 16S rRNA sequences are thus needed for accurate a nd reproducible taxonomy assignment in the study of marine bacterial communities.