Assessment of non-cultured aquatic fungal diversity from differenthabitats in Mexico
With the aim to explore the diversity of aquatic fungi in Mexico we present an investigation using a fragment of the 18S ribosomal DNA asa molecular marker obtained from different water bodies (marine, brackish and fresh water). Ribosomal gene fragments were obtained by DNAamplification, the resulti...
| Autores: | , , , , , , , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2016 |
| País: | México |
| Recursos: | Universidad Nacional Autónoma de México |
| Repositorio: | Redalyc-UNAM |
| OAI Identifier: | oai:redalyc.org:42546734003 |
| Acesso em linha: | https://www.redalyc.org/articulo.oa?id=42546734003 https://www.redalyc.org/journal/425/42546734003/ https://www.redalyc.org/journal/425/42546734003/html/ https://www.redalyc.org/journal/425/42546734003/42546734003.epub https://www.redalyc.org/journal/425/42546734003/movil |
| Access Level: | acceso abierto |
| Palavra-chave: | Biología Fungi Aquatic habitats |
| Resumo: | With the aim to explore the diversity of aquatic fungi in Mexico we present an investigation using a fragment of the 18S ribosomal DNA asa molecular marker obtained from different water bodies (marine, brackish and fresh water). Ribosomal gene fragments were obtained by DNAamplification, the resulting sequences were compared using multiple alignments against a collection of classified reference fungal sequences andthen subjected to phylogenetic clustering allowing the identification and classification of DNA sequences from environmental isolates as fungaldown to the family level, provided enough reference sequence were available. From our ensemble of 2,020 sequences identified as fungal, 23.8%were classified at the family level, 48.5% at the order level, 13% at the class/subphylum level and 14.7% of the sequences (all from the samesite) could not be unambiguously positioned in any of our reference fungal groups but were closely related to uncultivated marine fungi. Themost frequently recovered phylum was Ascomycota (89.1%), followed by Chytridiomycota (8.1%), Basidiomycota (2.8%) and Mucoromycotina(1.3%).All Rights Reserved © 2015 Universidad Nacional Autónoma de México, Instituto de Biología. This is an open access item distributed under theCreative Commons CC License BY-NC-ND 4.0. |
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