Genotoxic Effect of Amoxicillin on Peripheral Blood of Common Carp (Cyprinus carpio)

Amoxicillin (AMX), one of the most commonly prescribed antibiotics around the globe, has been found in diverse environmental water bodies, which have raised the concern of its possible toxicological effects on nontarget aquatic species. This study aimed to evaluate the potential genotoxic effects of...

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Detalles Bibliográficos
Autores: Armando Elizalde Velazquez, Gerardo Bernadac, Hector Martinez Rodriguez, Marcela Galar Martínez, Octavio Dublan, Leobardo Manuel Gómez Oliván
Tipo de recurso: capítulo de libro
Estado:Versión publicada
Fecha de publicación:2019
País:México
Institución:Universidad Autónoma de Ciudad Juárez
Repositorio:Repositorio Institucional de la Universidad Autónoma de Ciudad Juárez
OAI Identifier:oai:uacj.mx:oai:cathi.uacj.mx:20.500.11961ir-11717
Acceso en línea:https://doi.org/10.1007/978-3-030-27296-8_20
Access Level:acceso abierto
Palabra clave:Amoxicillin, Cyprinus carpio, Genotoxic, Comet assay, Amoxicilloic acid
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spelling Genotoxic Effect of Amoxicillin on Peripheral Blood of Common Carp (Cyprinus carpio)Armando Elizalde VelazquezGerardo BernadacHector Martinez RodriguezMarcela Galar MartínezOctavio DublanLeobardo Manuel Gómez OlivánAmoxicillin, Cyprinus carpio, Genotoxic, Comet assay, Amoxicilloic acidinfo:eu-repo/classification/cti/2Amoxicillin (AMX), one of the most commonly prescribed antibiotics around the globe, has been found in diverse environmental water bodies, which have raised the concern of its possible toxicological effects on nontarget aquatic species. This study aimed to evaluate the potential genotoxic effects of AMX in peripheral blood of the common carp Cyprinus carpio using the comet assay. Teleost fish were exposed to different concentrations (10 ng/L, 10 μg/L, and 10 mg/L) for different exposure times (12, 24, 48, 72, and 96 h). Additionally, the AMX and its main degradation product, amoxicilloic acid (AMA), were determined by high-performance liquid chromatography techniques coupled to a UV and an electrochemical detector (HPLC-EC). Results show a statically significant time concentration-dependent increase in DNA damage at 24 and 48 hours. No amoxicillin was detected at any time and at any concentration in any system tested, but amoxicilloic acid was detected and even quantified in water and blood since the beginning of the experiment. Amoxicilloic acid has the capacity to increase the amount of reactive oxygen species via hypersensitivity reactions, which are the responsible of the DNA damage in the blood lymphocytes of Cyprinus carpio.Springer, ChamArmando Elizalde VelazquezGerardo BernadacHector Martinez RodriguezMarcela Galar MartínezOctavio DublanLeobardo Manuel Gómez Oliván2019info:eu-repo/semantics/bookPartCapítulo de libroinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.1007/978-3-030-27296-8_20reponame:Repositorio Institucional de la Universidad Autónoma de Ciudad Juárezinstname:Universidad Autónoma de Ciudad Juárezinstacron:UACJen_USinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/4.0oai:uacj.mx:oai:cathi.uacj.mx:20.500.11961ir-117172025-11-26T19:47:03Z
dc.title.none.fl_str_mv Genotoxic Effect of Amoxicillin on Peripheral Blood of Common Carp (Cyprinus carpio)
title Genotoxic Effect of Amoxicillin on Peripheral Blood of Common Carp (Cyprinus carpio)
spellingShingle Genotoxic Effect of Amoxicillin on Peripheral Blood of Common Carp (Cyprinus carpio)
Armando Elizalde Velazquez
Amoxicillin, Cyprinus carpio, Genotoxic, Comet assay, Amoxicilloic acid
info:eu-repo/classification/cti/2
title_short Genotoxic Effect of Amoxicillin on Peripheral Blood of Common Carp (Cyprinus carpio)
title_full Genotoxic Effect of Amoxicillin on Peripheral Blood of Common Carp (Cyprinus carpio)
title_fullStr Genotoxic Effect of Amoxicillin on Peripheral Blood of Common Carp (Cyprinus carpio)
title_full_unstemmed Genotoxic Effect of Amoxicillin on Peripheral Blood of Common Carp (Cyprinus carpio)
title_sort Genotoxic Effect of Amoxicillin on Peripheral Blood of Common Carp (Cyprinus carpio)
dc.creator.none.fl_str_mv Armando Elizalde Velazquez
Gerardo Bernadac
Hector Martinez Rodriguez
Marcela Galar Martínez
Octavio Dublan
Leobardo Manuel Gómez Oliván
author Armando Elizalde Velazquez
author_facet Armando Elizalde Velazquez
Gerardo Bernadac
Hector Martinez Rodriguez
Marcela Galar Martínez
Octavio Dublan
Leobardo Manuel Gómez Oliván
author_role author
author2 Gerardo Bernadac
Hector Martinez Rodriguez
Marcela Galar Martínez
Octavio Dublan
Leobardo Manuel Gómez Oliván
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Armando Elizalde Velazquez
Gerardo Bernadac
Hector Martinez Rodriguez
Marcela Galar Martínez
Octavio Dublan
Leobardo Manuel Gómez Oliván
dc.subject.none.fl_str_mv Amoxicillin, Cyprinus carpio, Genotoxic, Comet assay, Amoxicilloic acid
info:eu-repo/classification/cti/2
topic Amoxicillin, Cyprinus carpio, Genotoxic, Comet assay, Amoxicilloic acid
info:eu-repo/classification/cti/2
description Amoxicillin (AMX), one of the most commonly prescribed antibiotics around the globe, has been found in diverse environmental water bodies, which have raised the concern of its possible toxicological effects on nontarget aquatic species. This study aimed to evaluate the potential genotoxic effects of AMX in peripheral blood of the common carp Cyprinus carpio using the comet assay. Teleost fish were exposed to different concentrations (10 ng/L, 10 μg/L, and 10 mg/L) for different exposure times (12, 24, 48, 72, and 96 h). Additionally, the AMX and its main degradation product, amoxicilloic acid (AMA), were determined by high-performance liquid chromatography techniques coupled to a UV and an electrochemical detector (HPLC-EC). Results show a statically significant time concentration-dependent increase in DNA damage at 24 and 48 hours. No amoxicillin was detected at any time and at any concentration in any system tested, but amoxicilloic acid was detected and even quantified in water and blood since the beginning of the experiment. Amoxicilloic acid has the capacity to increase the amount of reactive oxygen species via hypersensitivity reactions, which are the responsible of the DNA damage in the blood lymphocytes of Cyprinus carpio.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/bookPart
Capítulo de libro
info:eu-repo/semantics/publishedVersion
format bookPart
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1007/978-3-030-27296-8_20
url https://doi.org/10.1007/978-3-030-27296-8_20
dc.language.none.fl_str_mv en_US
language_invalid_str_mv en_US
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0
dc.publisher.none.fl_str_mv Springer, Cham
publisher.none.fl_str_mv Springer, Cham
dc.source.none.fl_str_mv reponame:Repositorio Institucional de la Universidad Autónoma de Ciudad Juárez
instname:Universidad Autónoma de Ciudad Juárez
instacron:UACJ
instname_str Universidad Autónoma de Ciudad Juárez
instacron_str UACJ
institution UACJ
reponame_str Repositorio Institucional de la Universidad Autónoma de Ciudad Juárez
collection Repositorio Institucional de la Universidad Autónoma de Ciudad Juárez
repository.name.fl_str_mv
repository.mail.fl_str_mv
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