Modificación de la célula nodriza de Trichinella spiralis en ratas Long Evans inmunizadas con antígeno soluble total de Trichinella spiralis y sacrificadas en diferentes tiempos
The purpose of this study was to evaluate the changes in the T. spiralis’ nurse cell in Long Evans rats immunized with Total Soluble Antigen T. spiralis sacrificed at different times. We worked with 25 male rats of two and a half months old, immunizing 20 of them. Then, the 25 rats were challenged w...
| Autores: | , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2012 |
| País: | México |
| Institución: | Universidad Autónoma de Zacatecas |
| Repositorio: | Repositorio Institucional Caxcán |
| Idioma: | español |
| OAI Identifier: | oai:http://ricaxcan.uaz.edu.mx:20.500.11845/837 |
| Acceso en línea: | http://localhost/xmlui/handle/20.500.11845/837 https://doi.org/10.48779/s6xj-xg32 https://doi.org/10.48779/jzcf-4c65 |
| Access Level: | acceso abierto |
| Palabra clave: | BIOLOGIA Y QUIMICA [2] Trichinella spiralis nurse cell changes TSA different times célula nodriza modificación AST diferentes tiempos |
| Sumario: | The purpose of this study was to evaluate the changes in the T. spiralis’ nurse cell in Long Evans rats immunized with Total Soluble Antigen T. spiralis sacrificed at different times. We worked with 25 male rats of two and a half months old, immunizing 20 of them. Then, the 25 rats were challenged with meat infected with T. spiralis, making the sacrifice of five rats each month, plus a rat control during 4 months to slaughter it; the techniques are carried out directly: on-board compression, artificial digestion and Hematoxylin- Eosin; indirect techniques of MIDD and Western Blot were applied for the serums. The result: a reduction in worm load. The maximum effects of protection were observed in the ultimate sacrifice after spending four months. The most obvious change to the nurse cell was a modification of the cyst and parasite’s spiral loss. Changes in the nurse cell of T. spiralis in Long Evans rats’ tissues immunized with AST and sacrificed at different times were evident with direct techniques of C/P, D/A and staining of H/E, we observed that the encystment was lost and the spiral was no longer viable, being statistically significant (P ≤ than 0.01). Key words: Trichinella spiralis, nurse cell, changes, TSA, different times. |
|---|