AISLAMIENTO, IDENTIFICACIÓN Y EVALUACIÓN DE UN CULTIVO MIXTO DE MICROORGANISMOS CON CAPACIDAD PARA DEGRADAR DDT
A mixed culture capable of degrading DDT was isolated from a mixture of samples taken from contaminated water, soil, and sediments from the Yaqui Valley in Sonora, México. The culturewas grown intermitently in a mineral salts medium suplemented with 133 ppm of centrifuged commercial DDT and incubate...
| Autores: | , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2011 |
| País: | México |
| Institución: | UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO |
| Repositorio: | Revista Internacional de Contaminación Ambiental |
| Idioma: | español |
| OAI Identifier: | oai:ojs.pkp.sfu.ca:article/23121 |
| Acceso en línea: | https://www.revistascca.unam.mx/rica/index.php/rica/article/view/23121 |
| Access Level: | acceso abierto |
| Palabra clave: | isolation biodegradation growth kinetics DDT aislamiento biodegradación cinética de crecimiento |
| Sumario: | A mixed culture capable of degrading DDT was isolated from a mixture of samples taken from contaminated water, soil, and sediments from the Yaqui Valley in Sonora, México. The culturewas grown intermitently in a mineral salts medium suplemented with 133 ppm of centrifuged commercial DDT and incubated at 28 º C and 150 rpm. Growth was evaluated meassuringprotein increase using the Lowry method. The protein containt was correlated to increase of dry mass weight. The culture had a specific growth rate of 0.072/h and a generation time of 9.62h. The residual DDT was determinated by gas chromatogrphy. The growth was sustained by DDT as a sole source of carbon and was completely consumed in the first 40 h. The metabolites DDD and DDE also present in commercial DDT were completely degraded without showing an increase in concentration throughout the culturing. The microbiological identification of this culture suggested that it was formed mainly by a Gramnegative bacilli. These bacilli were identified as members of the genera Pseudomonas, Neisseria, Moraxella andAcinetobacter. |
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