Genomic insights of a native bacterial consortium for wheat production sustainability
The use of plant growth-promoting bacteria as bioinoculants is a powerful tool to increase crop yield and quality and to improve nitrogen use efficiency (NUE) from fertilizers in plants. This study aimed to bioprospecting a native bacterial consortium ( Bacillus cabrialesii subsp. cabrialesii TE3 T...
| Authors: | , , , , , , , |
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| Format: | article |
| Status: | Published version |
| Publication Date: | 2024 |
| Country: | México |
| Institution: | Centro Internacional de Mejoramiento de Maíz y Trigo |
| Repository: | Repositorio Institucional de Publicaciones Multimedia del CIMMYT |
| OAI Identifier: | oai:repository.cimmyt.org:10883/34597 |
| Online Access: | https://hdl.handle.net/10883/34597 |
| Access Level: | Open access |
| Keyword: | AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Genomic Bioinformatic Analysis Wheat Crop Yield Wheat Quality Plant Growth-Promoting Bacteria NITROGEN-USE EFFICIENCY WHEAT CROP YIELD QUALITY PLANT GROWTH-PROMOTING MICROORGANISMS Wheat |
| Summary: | The use of plant growth-promoting bacteria as bioinoculants is a powerful tool to increase crop yield and quality and to improve nitrogen use efficiency (NUE) from fertilizers in plants. This study aimed to bioprospecting a native bacterial consortium ( Bacillus cabrialesii subsp. cabrialesii TE3 T , Priestia megaterium TRQ8, and Bacillus paralicheniformis TRQ65), through bioinformatic analysis, and to quantify the impact of its inoculation on NUE (measured through 15 N-isotopic techniques), grain yield, and grain quality of durum wheat variety CIRNO C2008 grown under three doses of urea (0, 120, and 240 kg N ha -1 ) during two consecutive agricultural cycles in the Yaqui Valley, Mexico. The inoculation of the bacterial consortium (BC) to the wheat crop, at a total N concentration of 123 -225 kg N ha -1 increased crop productivity and maintained grain quality, resulting in a yield increase of 1.1 ton ha -1 (6.0 vs. 7.1 ton ha -1 , 0 kg N ha -1 added, 123 kg N ha -1 in the soil) and of 2.0 ton ha -1 (5.9 vs. 7.9 ton ha -1 , 120 kg N ha -1 added, 104 kg N ha -1 in the soil) compared to the uninoculated controls at the same doses of N. The genomic bioinformatic analysis of the studied strains showed a great number of biofertilization-related genes regarding N and Fe acquisition, P assimilation, CO 2 fixation, Fe, P, and K solubilization, with important roles in agroecosystems, as well as genes related to the production of siderophores and stress response. A positive effect of the BC on NUE at the studied initial N content (123 and 104 kg N ha -1 ) was not observed. Nevertheless, increases of 14 % and 12.5 % on NUE (whole plant) were observed when 120 kg N ha -1 was applied compared to when wheat was fully fertilized (240 kg N ha -1 ). This work represents a link between bioinformatic approaches of a native bacterial inoculant and the quantification of its impact on durum wheat. |
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