Estudio químico biodirigido de la actividad antiasmática de Argemone platyceras
Present asthma research is focused on chronic inflammation and remodelling of airways. Leukotrienes take part in the remodelling process due to their direct effects on smooth muscle cells, eosinophils, epithelial cells, and other cell types. Leukotriene antagonists constitute a totally novel focus f...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2005 |
| País: | México |
| Institución: | Universidad Autónoma Metropolitana |
| Repositorio: | Repositorio Institucional de la UAM Iztapalapa |
| Idioma: | español |
| OAI Identifier: | oai:bindani.izt.uam.mx:pv63g0570 |
| Acceso en línea: | https://doi.org/10.24275/uami.pv63g0570 |
| Access Level: | acceso abierto |
| Palabra clave: | info:eu-repo/classification/LEM/Argemone platyceras info:eu-repo/classification/LEM/Traditional medicine info:eu-repo/classification/LEM/Asthma -- Treatment info:eu-repo/classification/LEM/Asma -- Tratamiento info:eu-repo/classification/LEM/Medicina tradicional info:eu-repo/classification/cti/3 |
| Sumario: | Present asthma research is focused on chronic inflammation and remodelling of airways. Leukotrienes take part in the remodelling process due to their direct effects on smooth muscle cells, eosinophils, epithelial cells, and other cell types. Leukotriene antagonists constitute a totally novel focus for processing inflammation by selective inhibition leukotriene effects. In the Mexican traditional medicine is a common practice the utilization of plant infusions or decoctions for the treatment of respiratory ailments and affections. The aims of the present study were to investigate the pharmacological antiasthmatic properties of Argemone platyceras Link & Otto (Papaveraceae) in guinea pig airways, and to identify the active principles through bioassay-guided fractionation. The methanol extract of the flowers produced a significant concentration-dependent rightward shift of the carbachol-induced response (10 y 30 µg/mL, P<0.01). In the histamine-induced response only Emax decreased when tissues were incubated with 30 and 100 µg/mL. The methanol extract of the leaves diminished pD2 values of the carbachol-induced response (10, 30, 100 µg/mL), and did not modify the histamine-induced response. The methanol extract of flowers and leaves of A. platyceras inhibited the contractile response to ovalbumin either in absence or presence of indomethacin in tracheae from sensitized guinea pigs. Three fractions were obtained from the leaf methanol extract: ethyl acetate (EtOAc), dichloromethane and water fractions. Only the EtOAc phase (100 µg/mL) of leaves and flowers significantly decreased pD2 of the contractile response induced by carbachol. The fractionation of EtOAc fractions from the leaves rendered seven secondary fractions. Only fractions 6 and 7 inhibited the contractile response induced by carbachol. Fraction 6 (100 µg/mL, P < 0.05) was more potent than fraction 7 (100 µg/mL) to inhibit the carbachol-induced response. Hence, fraction 6 was selected for further analysis. Fraction 6 significantly inhibited the contractile response to ovalbumin of tracheae from sensitized guinea pigs in absence (10 y 30 µg/mL, P<0.01) and in presence of indomethacin (3, 10 y 30 µg/mL, P<0.001). Incubation with fraction 6 (1, 3 and 10 µg/mL) produced a concentration-dependent rightward displacement and Emax decrease of contractile response to LTD4. This fraction inhibited completely the contractile response to LTD4 at 10 µg/mL. Fraction 6 was macerated with EtOAc obtaining an amorphous orange gum which was identified by its spectroscopic data as the glycosilated flavonoid isoquercitrin. Fraction 7 was macerated with EtOAc obtaining a yellow powder (116 mg) which was identified as the glycosilated flavonoid rutin. These results support the notion that A. platyceras contains compounds with anti-asthmatic properties. |
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