Micropropagation of chiltepín (Capsicum annuum L. cv. 'glabriusculum') by applying a pectic oligosaccharide
The aim of this study was to evaluate the effect of a pectic oligogalacturonide (PectiMorf®) in the micropropagation of chiltepin (Capsicum annuum L. cv. 'glabriusculum') and its adaptation to transplant. Multiple shoots were obtained by in vitro culture of nodal segments of 30-day-old pla...
| Autores: | , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2017 |
| País: | México |
| Institución: | UNIVERSIDAD DE GUANAJUATO |
| Repositorio: | Acta Universitaria |
| Idioma: | español |
| OAI Identifier: | oai:www.actauniversitaria.ugto.mx:article/1452 |
| Acceso en línea: | https://www.actauniversitaria.ugto.mx/index.php/acta/article/view/1452 |
| Access Level: | acceso abierto |
| Palabra clave: | Chiltepín micropropagation multiplication Pectimorf transplant ex vitro. micropropagación multiplicación trasplante ex vitro. |
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Micropropagation of chiltepín (Capsicum annuum L. cv. 'glabriusculum') by applying a pectic oligosaccharideMicropropagación de chiltepín (Capsicum annuum L. cv. ‘glabriusculum’) mediante el empleo de una oligosacarina de origen pécticoIzquierdo Oviedo, HumbertoAlcaraz Meléndez, LiliaRodríguez-Álvarez, MargaritoChiltepínmicropropagationmultiplicationPectimorftransplant ex vitro.ChiltepínmicropropagaciónmultiplicaciónPectimorftrasplante ex vitro.micropropagaciónThe aim of this study was to evaluate the effect of a pectic oligogalacturonide (PectiMorf®) in the micropropagation of chiltepin (Capsicum annuum L. cv. 'glabriusculum') and its adaptation to transplant. Multiple shoots were obtained by in vitro culture of nodal segments of 30-day-old plantlets from chiltepin, using a MS medium supplemented with BAP 10-5 M. The highest number of shoots, measuring fresh and dry mass per explant, was obtained in MS medium enriched with Pectimorf (1 mg.L1). At the time of lifting and reseting plantlets with Pectimorf (1 mg.L-1-10 mg.L1) in vitro to ex vitro, the survival rate was of 100%, which was maintained thereafter, obtaining a greater number and length of roots, as well as vigor, than in control plants and those treated with BAP. Also, plants obtained with these oligosaccharides generally showed higher content in chlorophyll α and β, and phaeopigments. The best results were achieved in plantlets treated with Pectimorf (10 mg.L-1). El objetivo de este trabajo fue evaluar el efecto de un oligogalacturónido de origen péctico (Pectimorf) en la micropropagación del chiltepín (Capsicum annuum L. cv. ‘glabriusculum’) y la adaptación al trasplante. Se indujeron brotes en cultivo in vitro de segmentos nodales de 30 días de edad provenientes de vitroplantas de chiltepín, obtenidas en un medio Murashigue Skoog (MS) suplementado con 6-BAP 10-5 M. El mayor número de brotes, masa fresca y seca por explante fue en medio MS enriquecido con Pectimorf (1 mg.L-1. En el momento de la transferencia de las plántulas obtenidas con Pectimorf (1 mg.L-1 - 10 mg.L-1) de las fases in vitro a la ex vitro, la supervivencia fue del 100%, lo cual se mantuvo con posterioridad. Asimismo, las plantas que se obtuvieron con esta oligosacarina, generalmente presentaron mayor contenido de clorofilas α, β y feopigmentos. Los mejores resultados fueron en las plántulas tratadas con Pectimorf (10 mg.L-1).Universidad de Guanajuato2017-11-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArtículo revisado por paresconservación de germoplasmaapplication/pdftext/htmlapplication/mswordapplication/xmlhttps://www.actauniversitaria.ugto.mx/index.php/acta/article/view/145210.15174/au.2017.1452Acta Universitaria; Vol. 27 Núm. 5 (2017); 34-43Acta Universitaria; Vol. 27 No. 5 (2017); 34-432007-96210188-6266reponame:Acta Universitariainstname:UNIVERSIDAD DE GUANAJUATOinstacron:UGTOspahttps://www.actauniversitaria.ugto.mx/index.php/acta/article/view/1452/pdfhttps://www.actauniversitaria.ugto.mx/index.php/acta/article/view/1452/htmlhttps://www.actauniversitaria.ugto.mx/index.php/acta/article/view/1452/2866https://www.actauniversitaria.ugto.mx/index.php/acta/article/view/1452/3149info:eu-repo/semantics/openAccessoai:www.actauniversitaria.ugto.mx:article/14522024-08-16T21:06:34Z |
| dc.title.none.fl_str_mv |
Micropropagation of chiltepín (Capsicum annuum L. cv. 'glabriusculum') by applying a pectic oligosaccharide Micropropagación de chiltepín (Capsicum annuum L. cv. ‘glabriusculum’) mediante el empleo de una oligosacarina de origen péctico |
| title |
Micropropagation of chiltepín (Capsicum annuum L. cv. 'glabriusculum') by applying a pectic oligosaccharide |
| spellingShingle |
Micropropagation of chiltepín (Capsicum annuum L. cv. 'glabriusculum') by applying a pectic oligosaccharide Izquierdo Oviedo, Humberto Chiltepín micropropagation multiplication Pectimorf transplant ex vitro. Chiltepín micropropagación multiplicación Pectimorf trasplante ex vitro. micropropagación |
| title_short |
Micropropagation of chiltepín (Capsicum annuum L. cv. 'glabriusculum') by applying a pectic oligosaccharide |
| title_full |
Micropropagation of chiltepín (Capsicum annuum L. cv. 'glabriusculum') by applying a pectic oligosaccharide |
| title_fullStr |
Micropropagation of chiltepín (Capsicum annuum L. cv. 'glabriusculum') by applying a pectic oligosaccharide |
| title_full_unstemmed |
Micropropagation of chiltepín (Capsicum annuum L. cv. 'glabriusculum') by applying a pectic oligosaccharide |
| title_sort |
Micropropagation of chiltepín (Capsicum annuum L. cv. 'glabriusculum') by applying a pectic oligosaccharide |
| dc.creator.none.fl_str_mv |
Izquierdo Oviedo, Humberto Alcaraz Meléndez, Lilia Rodríguez-Álvarez, Margarito |
| author |
Izquierdo Oviedo, Humberto |
| author_facet |
Izquierdo Oviedo, Humberto Alcaraz Meléndez, Lilia Rodríguez-Álvarez, Margarito |
| author_role |
author |
| author2 |
Alcaraz Meléndez, Lilia Rodríguez-Álvarez, Margarito |
| author2_role |
author author |
| dc.subject.none.fl_str_mv |
Chiltepín micropropagation multiplication Pectimorf transplant ex vitro. Chiltepín micropropagación multiplicación Pectimorf trasplante ex vitro. micropropagación |
| topic |
Chiltepín micropropagation multiplication Pectimorf transplant ex vitro. Chiltepín micropropagación multiplicación Pectimorf trasplante ex vitro. micropropagación |
| description |
The aim of this study was to evaluate the effect of a pectic oligogalacturonide (PectiMorf®) in the micropropagation of chiltepin (Capsicum annuum L. cv. 'glabriusculum') and its adaptation to transplant. Multiple shoots were obtained by in vitro culture of nodal segments of 30-day-old plantlets from chiltepin, using a MS medium supplemented with BAP 10-5 M. The highest number of shoots, measuring fresh and dry mass per explant, was obtained in MS medium enriched with Pectimorf (1 mg.L1). At the time of lifting and reseting plantlets with Pectimorf (1 mg.L-1-10 mg.L1) in vitro to ex vitro, the survival rate was of 100%, which was maintained thereafter, obtaining a greater number and length of roots, as well as vigor, than in control plants and those treated with BAP. Also, plants obtained with these oligosaccharides generally showed higher content in chlorophyll α and β, and phaeopigments. The best results were achieved in plantlets treated with Pectimorf (10 mg.L-1). |
| publishDate |
2017 |
| dc.date.none.fl_str_mv |
2017-11-06 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artículo revisado por pares conservación de germoplasma |
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article |
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publishedVersion |
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https://www.actauniversitaria.ugto.mx/index.php/acta/article/view/1452 10.15174/au.2017.1452 |
| url |
https://www.actauniversitaria.ugto.mx/index.php/acta/article/view/1452 |
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10.15174/au.2017.1452 |
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spa |
| language |
spa |
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https://www.actauniversitaria.ugto.mx/index.php/acta/article/view/1452/pdf https://www.actauniversitaria.ugto.mx/index.php/acta/article/view/1452/html https://www.actauniversitaria.ugto.mx/index.php/acta/article/view/1452/2866 https://www.actauniversitaria.ugto.mx/index.php/acta/article/view/1452/3149 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf text/html application/msword application/xml |
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Universidad de Guanajuato |
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Universidad de Guanajuato |
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Acta Universitaria; Vol. 27 Núm. 5 (2017); 34-43 Acta Universitaria; Vol. 27 No. 5 (2017); 34-43 2007-9621 0188-6266 reponame:Acta Universitaria instname:UNIVERSIDAD DE GUANAJUATO instacron:UGTO |
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