Sea turtle hatchling carapace as a source of high quantity and quality DNA

Hatchling DNA provides valuable information on sea turtles. Samples can be obtained from dead hatchlings or embryo, or, when live animal samples are needed, from blood, flipper or carapace. We compared 120 DNA extractions from flipper and carapace tissue of dead and live hatchlings. There were signi...

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Detalles Bibliográficos
Autores: Blanca I. González-Garza, L. Felipe Sánchez-Teyer, Omar Zapata-Pérez
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:México
Institución:Instituto Politécnico Nacional
Repositorio:Redalyc-IPN
OAI Identifier:oai:redalyc.org:47945599024
Acceso en línea:https://www.redalyc.org/articulo.oa?id=47945599024
Access Level:acceso abierto
Palabra clave:Ciencias de la Tierra
Carapace
sea turtle
DNA extraction
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spelling Sea turtle hatchling carapace as a source of high quantity and quality DNABlanca I. González-GarzaL. Felipe Sánchez-TeyerOmar Zapata-PérezCiencias de la TierraCarapacesea turtleDNA extractionHatchling DNA provides valuable information on sea turtles. Samples can be obtained from dead hatchlings or embryo, or, when live animal samples are needed, from blood, flipper or carapace. We compared 120 DNA extractions from flipper and carapace tissue of dead and live hatchlings. There were significant differences in DNA yields from the different tissues, but no significant differences in DNA purity. Some flipper samples yielded low amounts of DNA, while the carapace tissue consistently produced high yields of good quality DNA. This suggests that carapace represents the best option for tissue sampling of hatchling sea turtles in genetic research.Universidad de Valparaíso2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdf0717-3326https://www.redalyc.org/articulo.oa?id=47945599024Revista de Biología Marina y Oceanografía (Chile) Num.1 Vol.51reponame:Redalyc-IPNinstname:Instituto Politécnico Nacionalinstacron:IPNenhttp://www.redalyc.org/revista.oa?id=479Revista de Biología Marina y Oceanografíainfo:eu-repo/semantics/openAccessoai:redalyc.org:479455990242026-01-29T02:54:58Z
dc.title.none.fl_str_mv Sea turtle hatchling carapace as a source of high quantity and quality DNA
title Sea turtle hatchling carapace as a source of high quantity and quality DNA
spellingShingle Sea turtle hatchling carapace as a source of high quantity and quality DNA
Blanca I. González-Garza
Ciencias de la Tierra
Carapace
sea turtle
DNA extraction
title_short Sea turtle hatchling carapace as a source of high quantity and quality DNA
title_full Sea turtle hatchling carapace as a source of high quantity and quality DNA
title_fullStr Sea turtle hatchling carapace as a source of high quantity and quality DNA
title_full_unstemmed Sea turtle hatchling carapace as a source of high quantity and quality DNA
title_sort Sea turtle hatchling carapace as a source of high quantity and quality DNA
dc.creator.none.fl_str_mv Blanca I. González-Garza
L. Felipe Sánchez-Teyer
Omar Zapata-Pérez
author Blanca I. González-Garza
author_facet Blanca I. González-Garza
L. Felipe Sánchez-Teyer
Omar Zapata-Pérez
author_role author
author2 L. Felipe Sánchez-Teyer
Omar Zapata-Pérez
author2_role author
author
dc.subject.none.fl_str_mv Ciencias de la Tierra
Carapace
sea turtle
DNA extraction
topic Ciencias de la Tierra
Carapace
sea turtle
DNA extraction
description Hatchling DNA provides valuable information on sea turtles. Samples can be obtained from dead hatchlings or embryo, or, when live animal samples are needed, from blood, flipper or carapace. We compared 120 DNA extractions from flipper and carapace tissue of dead and live hatchlings. There were significant differences in DNA yields from the different tissues, but no significant differences in DNA purity. Some flipper samples yielded low amounts of DNA, while the carapace tissue consistently produced high yields of good quality DNA. This suggests that carapace represents the best option for tissue sampling of hatchling sea turtles in genetic research.
publishDate 2016
dc.date.none.fl_str_mv 2016
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dc.identifier.none.fl_str_mv 0717-3326
https://www.redalyc.org/articulo.oa?id=47945599024
identifier_str_mv 0717-3326
url https://www.redalyc.org/articulo.oa?id=47945599024
dc.language.none.fl_str_mv en
language_invalid_str_mv en
dc.relation.none.fl_str_mv http://www.redalyc.org/revista.oa?id=479
dc.rights.none.fl_str_mv Revista de Biología Marina y Oceanografía
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Revista de Biología Marina y Oceanografía
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad de Valparaíso
publisher.none.fl_str_mv Universidad de Valparaíso
dc.source.none.fl_str_mv Revista de Biología Marina y Oceanografía (Chile) Num.1 Vol.51
reponame:Redalyc-IPN
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