CHARACTERIZATION AND EXPRESSION OF THE CHITINASE CHIT II GENE FROM Lecanicillium lecanii IN SOLID-STATE FERMENTATION

Hydrolytic enzymes like chitinase are key factors in entomopathogenic fungi infections of insects. Chitinolytic activity of Lecanicillium lecanii was detected when growing in chitin and in grasshopper cuticle (Sphenarium purpurascens) in solidstate fermentation (SSF); the highest levels were reached...

Descripción completa

Detalles Bibliográficos
Autores: L. Mayorga-Reyes, E. Calderón-Garza, A. Gutiérrez-Nava, R. González-Cervantes, A. Azaola-Espinosa, E. Barranco-Florido
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:México
Institución:Universidad Autónoma Metropolitana
Repositorio:Redalyc-UAM
OAI Identifier:oai:redalyc.org:62024415008
Acceso en línea:https://www.redalyc.org/articulo.oa?id=62024415008
Access Level:acceso abierto
Palabra clave:Ingeniería
RT
PCR
solid
Chitinases
chitII gene
id MX_92ca7db656bfbcd84756ac6c45aeeb39
oai_identifier_str oai:redalyc.org:62024415008
network_acronym_str MX
network_name_str México
repository_id_str
spelling CHARACTERIZATION AND EXPRESSION OF THE CHITINASE CHIT II GENE FROM Lecanicillium lecanii IN SOLID-STATE FERMENTATIONL. Mayorga-ReyesE. Calderón-GarzaA. Gutiérrez-NavaR. González-CervantesA. Azaola-EspinosaE. Barranco-FloridoIngenieríaRTPCRsolidChitinaseschitII geneHydrolytic enzymes like chitinase are key factors in entomopathogenic fungi infections of insects. Chitinolytic activity of Lecanicillium lecanii was detected when growing in chitin and in grasshopper cuticle (Sphenarium purpurascens) in solidstate fermentation (SSF); the highest levels were reached when the fungus grew in chitin. Expression levels of chitII gene in L. lecanii depend on the carbon source present in the medium, and an increment in chitII gene expression was observed through fermentation time. Among the carbon sources evaluated, chitin showed the highest levels of chitII gene expression. When grown in glucose, basal levels of expression of chitII gene were detected, suggesting that the L. lecanii chitinolytic system is subject to an induction/repression mechanism. Electrophoresis in SDS-PAGE of a partially purified extract obtained from the growth on chitin and S. purpurascens cuticle revealed a band of 23 kDa approximately with ß-N-acetyl glucosaminidase activity in a zymogram analysis.Universidad Autónoma Metropolitana2012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdf1665-2738https://www.redalyc.org/articulo.oa?id=62024415008Revista Mexicana de Ingeniería Química (México) Num.1 Vol.11reponame:Redalyc-UAMinstname:Universidad Autónoma Metropolitanainstacron:UAMenhttp://www.redalyc.org/revista.oa?id=620Revista Mexicana de Ingeniería Químicainfo:eu-repo/semantics/openAccessoai:redalyc.org:620244150082025-03-05T19:09:43Z
dc.title.none.fl_str_mv CHARACTERIZATION AND EXPRESSION OF THE CHITINASE CHIT II GENE FROM Lecanicillium lecanii IN SOLID-STATE FERMENTATION
title CHARACTERIZATION AND EXPRESSION OF THE CHITINASE CHIT II GENE FROM Lecanicillium lecanii IN SOLID-STATE FERMENTATION
spellingShingle CHARACTERIZATION AND EXPRESSION OF THE CHITINASE CHIT II GENE FROM Lecanicillium lecanii IN SOLID-STATE FERMENTATION
L. Mayorga-Reyes
Ingeniería
RT
PCR
solid
Chitinases
chitII gene
title_short CHARACTERIZATION AND EXPRESSION OF THE CHITINASE CHIT II GENE FROM Lecanicillium lecanii IN SOLID-STATE FERMENTATION
title_full CHARACTERIZATION AND EXPRESSION OF THE CHITINASE CHIT II GENE FROM Lecanicillium lecanii IN SOLID-STATE FERMENTATION
title_fullStr CHARACTERIZATION AND EXPRESSION OF THE CHITINASE CHIT II GENE FROM Lecanicillium lecanii IN SOLID-STATE FERMENTATION
title_full_unstemmed CHARACTERIZATION AND EXPRESSION OF THE CHITINASE CHIT II GENE FROM Lecanicillium lecanii IN SOLID-STATE FERMENTATION
title_sort CHARACTERIZATION AND EXPRESSION OF THE CHITINASE CHIT II GENE FROM Lecanicillium lecanii IN SOLID-STATE FERMENTATION
dc.creator.none.fl_str_mv L. Mayorga-Reyes
E. Calderón-Garza
A. Gutiérrez-Nava
R. González-Cervantes
A. Azaola-Espinosa
E. Barranco-Florido
author L. Mayorga-Reyes
author_facet L. Mayorga-Reyes
E. Calderón-Garza
A. Gutiérrez-Nava
R. González-Cervantes
A. Azaola-Espinosa
E. Barranco-Florido
author_role author
author2 E. Calderón-Garza
A. Gutiérrez-Nava
R. González-Cervantes
A. Azaola-Espinosa
E. Barranco-Florido
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ingeniería
RT
PCR
solid
Chitinases
chitII gene
topic Ingeniería
RT
PCR
solid
Chitinases
chitII gene
description Hydrolytic enzymes like chitinase are key factors in entomopathogenic fungi infections of insects. Chitinolytic activity of Lecanicillium lecanii was detected when growing in chitin and in grasshopper cuticle (Sphenarium purpurascens) in solidstate fermentation (SSF); the highest levels were reached when the fungus grew in chitin. Expression levels of chitII gene in L. lecanii depend on the carbon source present in the medium, and an increment in chitII gene expression was observed through fermentation time. Among the carbon sources evaluated, chitin showed the highest levels of chitII gene expression. When grown in glucose, basal levels of expression of chitII gene were detected, suggesting that the L. lecanii chitinolytic system is subject to an induction/repression mechanism. Electrophoresis in SDS-PAGE of a partially purified extract obtained from the growth on chitin and S. purpurascens cuticle revealed a band of 23 kDa approximately with ß-N-acetyl glucosaminidase activity in a zymogram analysis.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 1665-2738
https://www.redalyc.org/articulo.oa?id=62024415008
identifier_str_mv 1665-2738
url https://www.redalyc.org/articulo.oa?id=62024415008
dc.language.none.fl_str_mv en
language_invalid_str_mv en
dc.relation.none.fl_str_mv http://www.redalyc.org/revista.oa?id=620
dc.rights.none.fl_str_mv Revista Mexicana de Ingeniería Química
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Revista Mexicana de Ingeniería Química
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Autónoma Metropolitana
publisher.none.fl_str_mv Universidad Autónoma Metropolitana
dc.source.none.fl_str_mv Revista Mexicana de Ingeniería Química (México) Num.1 Vol.11
reponame:Redalyc-UAM
instname:Universidad Autónoma Metropolitana
instacron:UAM
instname_str Universidad Autónoma Metropolitana
instacron_str UAM
institution UAM
reponame_str Redalyc-UAM
collection Redalyc-UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1858176147921043456
score 14.964252

Similares en LA Referencia