Influencia de nicotinamida sobre el perfil de lípidos y su repercusión en la actividad de esterasas circulantes en un modelo de síndrome metabólico
Acetylcholinesterase, butyrylcholinesterase and paraoxonase - 1 are glycoproteins that besides regulating the neuromuscular cholinergic system and detoxify the body its activities are risk factors associated with metabolic syndrome (MS). The objective was to evaluate the participation of nicotinamid...
| Autor: | |
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| Tipo de recurso: | tesis de maestría |
| Estado: | Versión publicada |
| Fecha de publicación: | 2016 |
| País: | México |
| Institución: | Universidad Autónoma Metropolitana |
| Repositorio: | Repositorio Institucional de la UAM Iztapalapa |
| Idioma: | español |
| OAI Identifier: | oai:bindani.izt.uam.mx:9c67wm855 |
| Acceso en línea: | https://doi.org/10.24275/uami.9c67wm855 |
| Access Level: | acceso abierto |
| Palabra clave: | info:eu-repo/classification/LEM/Metabolic syndrome info:eu-repo/classification/LEM/Síndrome metabólico info:eu-repo/classification/LEM/Glicoproteínas info:eu-repo/classification/LEM/Glycoproteins info:eu-repo/classification/cti/2 |
| Sumario: | Acetylcholinesterase, butyrylcholinesterase and paraoxonase - 1 are glycoproteins that besides regulating the neuromuscular cholinergic system and detoxify the body its activities are risk factors associated with metabolic syndrome (MS). The objective was to evaluate the participation of nicotinamide (NAM) on the activity and glycosylation pattern of circulating esterases in a model of metabolic syndrome. Male rats divided into six groups received the following treatments: 1) Control, 2) NAM5mM, 3) Fructose, 4) F - NAM5mM 5) F - NAM10mM, 6) F - NAM15mM. 40% fructose was administered in the drinking water ad libitum for 16 weeks; 30 days after treatment began NAM (5, 10 and 15 mM) in drinking water for 5 hr daily. The parameters evaluated were, body weight, tolerance test glucose, blood pressure, lipid profile, transaminases, specific activity of circulating esterase and cholinesterase glycosylation pattern by interaction with lectins of different specificity. Fructose increased cholesterol, triacylglycerols, fatty acids, transaminases and blood pressure. And the activities of both cholinesterases and also decreased the activity of paraoxonase - 1 whole relative to the control group. In contrast, NAM decreased metabolic alterations and cholinesterase activities with similar values to the control. Besides increasing paraoxonase - 1 activity. Highlights glycosylation pattern for acetylcholinesterase was decreased to 80% fructose interaction with lectin wheat compared to the control, 15% of NAM recovering interaction. For butyrylcholinesterase, it decreased 50% fructose interaction with lentil lectin compared to the control, which was restored by NAM. In conclusion; fructose decreased the activity of paraoxonase - 1 and increase cholinesterase activity in addition to modifying its glycosylation prevents incorporation lentil lectin to mannose residues present in the protein, and the incorporation of lectin wheat residues of N - acetylglucosamine and sialic acid, NAM restored these changes given by the SM. These structural variations of oligosaccharides present in the cholinesterases contribute to its folding, stability and biological function. The diet is considered an exogenous factor responsible for modulating the protector and antioxidant paraoxonase - 1 the effect. |
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