Effects of sporulation conditions on the germination and germination protein levels of bacillus subtilis spores
Bacillus subtilis spores prepared in rich medium germinated faster with nutrient germinants than poor-medium spores as populations in liquid and multiple individual spores on a microscope slide. Poor-medium spores had longer average lag times between mixing of spores with nutrient germinants and ini...
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2012 |
| País: | México |
| Recursos: | Universidad Autónoma de Guerrero |
| Repositorio: | Repositorio Institucional de Ciencia Abierta de la Universidad Autónoma de Guerrero |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.uagro.mx:uagro/403 |
| Acesso em linha: | http://ri.uagro.mx/handle/uagro/403 http://dx.doi.org/doi:10.1128/AEM.07908-11 |
| Access Level: | acceso abierto |
| Palavra-chave: | MEDICINA Y CIENCIAS DE LA SALUD::CIENCIAS MÉDICAS::BIOQUÍMICA |
| Resumo: | Bacillus subtilis spores prepared in rich medium germinated faster with nutrient germinants than poor-medium spores as populations in liquid and multiple individual spores on a microscope slide. Poor-medium spores had longer average lag times between mixing of spores with nutrient germinants and initiation of Ca-dipicolinic acid (CaDPA) release. Rich-medium spores made at 37C germinated slightly faster with nutrient germinants than 23C spores in liquid, but not when spores germinated on a slide. The difference in germination characteristics of these spore populations in liquid was paralleled by changes in expression levels of a transcriptional lacZ fusion to the gerA operon, encoding a germinant receptor (GR). |
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