BIODEGRADATION OF THE COMMERCIAL MIXTURE OF THE HERBICIDES ATRAZINE AND AMETRYN, USING CULTURES WITH SUSPENDED AND IMMOBILIZED CELLS

The excessive use of herbicides has caused weeds resistance to these compounds; thus, it has been implemented the use of mixtures of herbicides to ensure its effect on weeds. One of the commercial mixtures used in Mexico is that of atrazine and ametryn. Information on processes for ametryn degradati...

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Autores: Castillo-Martínez, Karla Lizzette, Ahuatzi-Chacón, Deifilia, Ruiz-Ordaz, Nora, Sandoval-Carrasco, Carlos Alberto, Juárez-Ramírez, Cleotilde, Galíndez-Mayer, Juvencio, Salmerón-Alcocer, Angélica María
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:México
Institución:UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO
Repositorio:Revista Internacional de Contaminación Ambiental
Idioma:español
OAI Identifier:oai:ojs.pkp.sfu.ca:article/53091
Acceso en línea:https://www.revistascca.unam.mx/rica/index.php/rica/article/view/RICA.2019.35.03.05
Access Level:acceso abierto
Palabra clave:quimiostato
biobarrera
comunidad microbiana
biopelícula
chemostat
biobarrier
microbial community
biofilm
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dc.title.none.fl_str_mv BIODEGRADATION OF THE COMMERCIAL MIXTURE OF THE HERBICIDES ATRAZINE AND AMETRYN, USING CULTURES WITH SUSPENDED AND IMMOBILIZED CELLS
DEGRADACIÓN DE LA MEZCLA COMERCIAL DE LOS HERBICIDAS ATRAZINA Y AMETRINA, UTILIZANDO CULTIVOS CON CÉLULAS EN SUSPENSIÓN E INMOVILIZADAS
title BIODEGRADATION OF THE COMMERCIAL MIXTURE OF THE HERBICIDES ATRAZINE AND AMETRYN, USING CULTURES WITH SUSPENDED AND IMMOBILIZED CELLS
spellingShingle BIODEGRADATION OF THE COMMERCIAL MIXTURE OF THE HERBICIDES ATRAZINE AND AMETRYN, USING CULTURES WITH SUSPENDED AND IMMOBILIZED CELLS
Castillo-Martínez, Karla Lizzette
quimiostato
biobarrera
comunidad microbiana
biopelícula
chemostat
biobarrier
microbial community
biofilm
title_short BIODEGRADATION OF THE COMMERCIAL MIXTURE OF THE HERBICIDES ATRAZINE AND AMETRYN, USING CULTURES WITH SUSPENDED AND IMMOBILIZED CELLS
title_full BIODEGRADATION OF THE COMMERCIAL MIXTURE OF THE HERBICIDES ATRAZINE AND AMETRYN, USING CULTURES WITH SUSPENDED AND IMMOBILIZED CELLS
title_fullStr BIODEGRADATION OF THE COMMERCIAL MIXTURE OF THE HERBICIDES ATRAZINE AND AMETRYN, USING CULTURES WITH SUSPENDED AND IMMOBILIZED CELLS
title_full_unstemmed BIODEGRADATION OF THE COMMERCIAL MIXTURE OF THE HERBICIDES ATRAZINE AND AMETRYN, USING CULTURES WITH SUSPENDED AND IMMOBILIZED CELLS
title_sort BIODEGRADATION OF THE COMMERCIAL MIXTURE OF THE HERBICIDES ATRAZINE AND AMETRYN, USING CULTURES WITH SUSPENDED AND IMMOBILIZED CELLS
dc.creator.none.fl_str_mv Castillo-Martínez, Karla Lizzette
Ahuatzi-Chacón, Deifilia
Ruiz-Ordaz, Nora
Sandoval-Carrasco, Carlos Alberto
Juárez-Ramírez, Cleotilde
Galíndez-Mayer, Juvencio
Salmerón-Alcocer, Angélica María
author Castillo-Martínez, Karla Lizzette
author_facet Castillo-Martínez, Karla Lizzette
Ahuatzi-Chacón, Deifilia
Ruiz-Ordaz, Nora
Sandoval-Carrasco, Carlos Alberto
Juárez-Ramírez, Cleotilde
Galíndez-Mayer, Juvencio
Salmerón-Alcocer, Angélica María
author_role author
author2 Ahuatzi-Chacón, Deifilia
Ruiz-Ordaz, Nora
Sandoval-Carrasco, Carlos Alberto
Juárez-Ramírez, Cleotilde
Galíndez-Mayer, Juvencio
Salmerón-Alcocer, Angélica María
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv quimiostato
biobarrera
comunidad microbiana
biopelícula
chemostat
biobarrier
microbial community
biofilm
topic quimiostato
biobarrera
comunidad microbiana
biopelícula
chemostat
biobarrier
microbial community
biofilm
description The excessive use of herbicides has caused weeds resistance to these compounds; thus, it has been implemented the use of mixtures of herbicides to ensure its effect on weeds. One of the commercial mixtures used in Mexico is that of atrazine and ametryn. Information on processes for ametryn degradation is scarce, and virtually no references to the biodegradation of the commercial mixture of atrazine and ametryn exist. In this study, the biodegradation of this commercial mixture using a microbial community in different types of cultures, and using a chemostat and biobarrier is presented. To assess the degradation capability of the microbial community, planktonic cells were used in a semi-continuous batch system. From this system, the continuous process was started. In repeated batch cultures, complete removal of both herbicides was obtained. In the continuous system, at all tested volumetric loads, the removal efficiencies of ametryn and atrazine were of 100 and 97 % respectively. During continuous operation, biofilm formation on the reactor walls was observed; thus, it was decided to install a biobarrier using volcanic rock fragments (tezontle) as biofilm support. In this system, the removal efficiencies (ŋ) and the volumetric removal rates (Rv) of the herbicide mixture were 100 % and 287 mg/L/d, respectively. At all the loading rates tested, cyanuric acid accumulation was observed; however, when the biobarrier was operated at low volumetric loading rates, considerable metabolic degradation of this intermediate was achieved.
publishDate 2019
dc.date.none.fl_str_mv 2019-07-31
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
ART
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.revistascca.unam.mx/rica/index.php/rica/article/view/RICA.2019.35.03.05
10.20937/RICA.2019.35.03.05
url https://www.revistascca.unam.mx/rica/index.php/rica/article/view/RICA.2019.35.03.05
identifier_str_mv 10.20937/RICA.2019.35.03.05
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://www.revistascca.unam.mx/rica/index.php/rica/article/view/RICA.2019.35.03.05/46871
dc.rights.none.fl_str_mv Derechos de autor 2019 Revista Internacional de Contaminación Ambiental
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2019 Revista Internacional de Contaminación Ambiental
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto de Ciencias de la Atmósfera, Universidad Nacional Autónoma de México
publisher.none.fl_str_mv Instituto de Ciencias de la Atmósfera, Universidad Nacional Autónoma de México
dc.source.none.fl_str_mv Revista Internacional de Contaminación Ambiental; Vol. 35 Núm. 3 (2019); 575-588
Revista Internacional de Contaminación Ambiental; Vol. 35 No. 3 (2019); 575-588
0188-4999
reponame:Revista Internacional de Contaminación Ambiental
instname:UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO
instacron:UNAM
instname_str UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO
instacron_str UNAM
institution UNAM
reponame_str Revista Internacional de Contaminación Ambiental
collection Revista Internacional de Contaminación Ambiental
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling BIODEGRADATION OF THE COMMERCIAL MIXTURE OF THE HERBICIDES ATRAZINE AND AMETRYN, USING CULTURES WITH SUSPENDED AND IMMOBILIZED CELLSDEGRADACIÓN DE LA MEZCLA COMERCIAL DE LOS HERBICIDAS ATRAZINA Y AMETRINA, UTILIZANDO CULTIVOS CON CÉLULAS EN SUSPENSIÓN E INMOVILIZADASCastillo-Martínez, Karla LizzetteAhuatzi-Chacón, DeifiliaRuiz-Ordaz, NoraSandoval-Carrasco, Carlos AlbertoJuárez-Ramírez, CleotildeGalíndez-Mayer, JuvencioSalmerón-Alcocer, Angélica Maríaquimiostatobiobarreracomunidad microbianabiopelículachemostatbiobarriermicrobial communitybiofilmThe excessive use of herbicides has caused weeds resistance to these compounds; thus, it has been implemented the use of mixtures of herbicides to ensure its effect on weeds. One of the commercial mixtures used in Mexico is that of atrazine and ametryn. Information on processes for ametryn degradation is scarce, and virtually no references to the biodegradation of the commercial mixture of atrazine and ametryn exist. In this study, the biodegradation of this commercial mixture using a microbial community in different types of cultures, and using a chemostat and biobarrier is presented. To assess the degradation capability of the microbial community, planktonic cells were used in a semi-continuous batch system. From this system, the continuous process was started. In repeated batch cultures, complete removal of both herbicides was obtained. In the continuous system, at all tested volumetric loads, the removal efficiencies of ametryn and atrazine were of 100 and 97 % respectively. During continuous operation, biofilm formation on the reactor walls was observed; thus, it was decided to install a biobarrier using volcanic rock fragments (tezontle) as biofilm support. In this system, the removal efficiencies (ŋ) and the volumetric removal rates (Rv) of the herbicide mixture were 100 % and 287 mg/L/d, respectively. At all the loading rates tested, cyanuric acid accumulation was observed; however, when the biobarrier was operated at low volumetric loading rates, considerable metabolic degradation of this intermediate was achieved.El empleo desmedido de los herbicidas ha ocasionado que las malezas generen resistencia a estos agroquímicos, por lo que se ha implementado el uso de mezclas para garantizar su efecto sobre diversos pastos indeseables. Una de las formulaciones comerciales utilizada en México es la mezcla de atrazina con ametrina. La información sobre procesos de degradación de la ametrina es escasa y prácticamente no hay referencias sobre la biodegradación de la mezcla comercial de la atrazina con ametrina. En este trabajo se presenta la biodegradación de esta mezcla comercial utilizando una comunidad microbiana en diferentes tipos de cultivo, empleando un quimiostato y una biobarrera. Para evaluar la capacidad de degradación de la comunidad microbiana se utilizaron células planctónicas en un sistema por lote semicontinuo, donde se obtuvo la remoción completa de ambos herbicidas. A partir de este sistema se inició un proceso continuo en el que, con todas las cargas volumétricas probadas, se removió el 100 % de ametrina y más del 97 % de atrazina. Durante la operación del proceso continuo se observó la formación de biopelícula en las paredes del reactor, por lo que se decidió utilizar una biobarrera con fragmentos de roca volcánica (tezontle) como soporte. En este sistema se obtuvieron eficiencias (ŋ) y velocidades volumétricas de remoción (RV) de los herbicidas, del 100 % y 287 mg/L/d, respectivamente. En todos los cultivos evaluados se observó la acumulación de ácido cianúrico; sin embargo, a bajas cargas volumétricas se alcanzó una degradación considerable de este intermediario metabólico. Instituto de Ciencias de la Atmósfera, Universidad Nacional Autónoma de México2019-07-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionARTapplication/pdfhttps://www.revistascca.unam.mx/rica/index.php/rica/article/view/RICA.2019.35.03.0510.20937/RICA.2019.35.03.05Revista Internacional de Contaminación Ambiental; Vol. 35 Núm. 3 (2019); 575-588Revista Internacional de Contaminación Ambiental; Vol. 35 No. 3 (2019); 575-5880188-4999reponame:Revista Internacional de Contaminación Ambientalinstname:UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICOinstacron:UNAMspahttps://www.revistascca.unam.mx/rica/index.php/rica/article/view/RICA.2019.35.03.05/46871Derechos de autor 2019 Revista Internacional de Contaminación Ambientalinfo:eu-repo/semantics/openAccessoai:ojs.pkp.sfu.ca:article/530912024-08-16T19:00:38Z
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