Evaluación de la interacción de la proteína de matriz del virus sincitial respiratorio humano (VSRH) con la proteína nucleolar fibrilarina
The Human Respiratory Syncytial Virus (HRSV) is one of the main etiological agents causing respiratory infections in infants. However, reinfections can occur throughout life. Therefore, HRSV is often a public health problem that affects various age groups. That is why generating information about th...
| Autor: | |
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| Tipo de recurso: | tesis de maestría |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | México |
| Institución: | Universidad Autónoma Metropolitana |
| Repositorio: | Repositorio Institucional de la UAM Iztapalapa |
| Idioma: | español |
| OAI Identifier: | oai:bindani.izt.uam.mx:02870w212 |
| Acceso en línea: | https://doi.org/10.24275/uami.02870w212 |
| Access Level: | acceso abierto |
| Palabra clave: | info:eu-repo/classification/LEM/Respiratory syncytial virus info:eu-repo/classification/LEM/Respiratory organs -- Diseases info:eu-repo/classification/LEM/Aparato respiratorio -- Enfermedades info:eu-repo/classification/LEM/Virus sincitial respiratorio info:eu-repo/classification/cti/3 |
| Sumario: | The Human Respiratory Syncytial Virus (HRSV) is one of the main etiological agents causing respiratory infections in infants. However, reinfections can occur throughout life. Therefore, HRSV is often a public health problem that affects various age groups. That is why generating information about the mechanisms of viral infection becomes necessary in order to be able to offer pharmacological treatments that allow patients to have a moderate infection without clinical complications. One of the virus-host interaction mechanisms studied in recent years in HRSV is the translocation of viral proteins to the nucleus. In this sense, during the first hours of HRSV infection, the matrix protein and the NS1 protein can be found in the nucleus to modulate the expression of genes that allow the virus to continue its infection cycle in a more efficient way. Although there is already a mechanism by which the matrix protein can regulate cell transcription, we decided to explore another possible mechanism by which said protein could regulate the said process. Therefore, our work focused on evaluating the subcellular distribution of the matrix protein in infected A549 cells, as well as analysis of the possible interaction of the matrix protein with the fibrillary protein, a protein highly related to cell transcription. During the first hours of HRSV infection, the matrix protein presented a perinuclear and cytoplasmic distribution, not a nuclear one, as previously reported. When performing the analysis of the colocalization of the matrix protein and fibrillarin, the colocalization did not occur. Therefore it is not present. However, we observed that the subcellular distribution of fibrillarin protein was the same in mockinfected and infected cells. Finally, when performing an in-silico analysis, our data indicate that the interaction of these two mentioned proteins is possibly not viable. Although all our data were not as expected, there are still unknowns that may allow us to explore new viral mechanisms in the future. |
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