Optimization of the diaphonization and staining technique of Piovesana (2014), applied to the fish Gymnocorymbus ternetzi

The diaphonization technique consists in making the soft tissues of an organism transparent in order to observe the bone and cartilaginous tissues. Specific dyes are used for these purposes, mainly red alizarin for bone. There are no standardized protocols for each species, since it is generalized f...

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Detalles Bibliográficos
Autores: Romero-Oliva, Oscar Jesús, González-Rodríguez , Katia Adriana
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:México
Institución:UNIVERSIDAD AUTÓNOMA DEL ESTADO DE HIDALGO
Repositorio:PÄDI Boletín Científico de Ciencias Básicas e Ingeniería del ICBI
Idioma:español
OAI Identifier:oai:repository.uaeh.edu.mx:article/3424
Acceso en línea:https://repository.uaeh.edu.mx/revistas/index.php/icbi/article/view/3424
Access Level:acceso abierto
Palabra clave:alizarin red
Diaphonization
Gymnocorymbus ternetzi
Diafanización
Rojo de alizarina
Descripción
Sumario:The diaphonization technique consists in making the soft tissues of an organism transparent in order to observe the bone and cartilaginous tissues. Specific dyes are used for these purposes, mainly red alizarin for bone. There are no standardized protocols for each species, since it is generalized for large groups, being the group of fishes one of the least defined. In this article a protocol of the technique was developed for the nun fish (Gymnocorymbus ternetzi), there are included, the necessary chemicals, and the costs in Mexican pesos.