Inhibición de la agregación plaquetaria por poliaminas en conejos Nueva Zelanda con hipercolesterolemia inducida
Atherosclerosis is one of the main causes of death worldwide. One of the most commonly used animal model for the study of atherogenesis and the effect of several substances on plaque formation is the New Zealand rabbit fed with a cholesterol enriched diet. In this study the formation of atherosclero...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2000 |
| País: | México |
| Institución: | Universidad Autónoma Metropolitana |
| Repositorio: | Repositorio Institucional de la UAM Iztapalapa |
| Idioma: | español |
| OAI Identifier: | oai:bindani.izt.uam.mx:kh04dq32v |
| Acceso en línea: | https://doi.org/10.24275/uami.kh04dq32v |
| Access Level: | acceso abierto |
| Palabra clave: | info:eu-repo/classification/LEM/Atherosclerosis info:eu-repo/classification/LEM/Agregación plaquetaria info:eu-repo/classification/LEM/Aterosclerosis info:eu-repo/classification/cti/3 |
| Sumario: | Atherosclerosis is one of the main causes of death worldwide. One of the most commonly used animal model for the study of atherogenesis and the effect of several substances on plaque formation is the New Zealand rabbit fed with a cholesterol enriched diet. In this study the formation of atherosclerotic plaques was studied in New Zealand rabbits fed with 1% and 10% cholesterol. Serum lipids and glucose were evaluated and the presence of atherosclerotic lesions was studied histologically. In rabbits fed with 10% cholesterol, serum total cholesterol increased from 26.3 k 8.1 mg/dl to 1485.0 k 26.8 mg/dl (p <0.05). LDL-cholesterol also increased from 15.9k5.9 to 1383.8 k 58.9 (p c0.5); and triglycerides from 88.3 * 35.6 to 411.0 * 154.5. After 10 weeks some lesions were found in the aorta of cholesterol-fed rabbits In this work we used the hypercholesterolemic rabbit to evaluate the effect of L-arginine and polyamines on platelet aggregation, which is closely associated to plaque formation. Polyamines are aliphatic molecules that present diverse citoprotective, immunoregulatory and antioxidant activities in addition to their antagonist effect on platelet aggregation in rat and human. The antiaggregating effect of polyamines putrescine, spermidine and spermine was studied in platelets obtained from normal and hypercholesterolemic rabbits (fed with 10% cholesterol). Platelet rich plasma was used for the platelet aggregation assay in the presence of 2.5 pmoledml of ADP or 2 pg/ml collagen as aggregating agents. A 75% inhibition was observed with 10 pM polyamines in control rabbits. Spermine inhibited platelet aggregation by 54% in hypercholesterolemic rabbits using ADP as agonist. The order of effectivenes was spermine> spermidine>putrescine. In addition, arginase activity and L-arginine content was evaluated in PRP. These parameters were not altered by hypercholesterolemia. Our results demonstrate that polyamines may have a role in the regulation of platelet aggregation both in normal conditions and in hypercholesterolemia. |
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