Development of an advanced biophotonic platform for studies and applications with live biological samples
A biophotonic platform incorporating various optical techniques into a single system was designed, built, and characterized. The primary goal of building this kind of system was to study living cells. The techniques incorporated as modules into an inverted optical microscope were optical trapping wi...
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| Formato: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2021 |
| País: | México |
| Recursos: | Centro de Investigación Científica y de Educación Superior de Ensenada |
| Repositorio: | Repositorio Institucional CICESE |
| Idioma: | inglés |
| OAI Identifier: | oai:cicese.repositorioinstitucional.mx:1007/3626 |
| Acesso em linha: | http://cicese.repositorioinstitucional.mx/jspui/handle/1007/3626 |
| Access Level: | acceso abierto |
| Palavra-chave: | info:eu-repo/classification/Autor/optical trapping, microalgae, photostimulus info:eu-repo/classification/Autor/atrapamiento óptico, microalgas, fotoestímulo info:eu-repo/classification/cti/7 info:eu-repo/classification/cti/33 info:eu-repo/classification/cti/3311 info:eu-repo/classification/cti/331111 |
| Resumo: | A biophotonic platform incorporating various optical techniques into a single system was designed, built, and characterized. The primary goal of building this kind of system was to study living cells. The techniques incorporated as modules into an inverted optical microscope were optical trapping with a single trap or dynamically reconfigurable multiple traps, back focal plane interferometry, and fluorescence microscopy. An integrated instrument control program with a graphical user interface was developed to maximize the system’s capabilities and simplify data acquisition. It allowed the simultaneous or independent use of the optical techniques. The system was used to study green biflagellated microalgae Dunaliella tertiolecta placed in a 50 μm thick glass chamber. Optical trapping without apparent photodamage of this highly motile microorganism was possible with a 1064 nm wavelength laser. Excitation of the chlorophyll fluorescence in the microalgae’s body was done with a 405 nm wavelength laser. Back focal plane interferometry was applied to record the cell’s motility, obtaining the rotation frequency of the body and flagella as the microalgae were held in the optical trap. One of the main results of using the developed biophotonic platform was the in vivo observation of laser-induced detachment of Dunaliella tertiolecta from a glass surface, providing some insight into the photostimulus produced by near-infrared light on this species. The biophotonic platform techniques were tested individually and imultaneously, demonstrating that the developed system can be a reliable tool to study live cells. |
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