Molecular genetic diversity and population structure of Ethiopian white lupin landraces: implications for breeding and conservation

White lupin is one of the four economically important species of the Lupinus genus and is an important grain legume in the Ethiopian farming system. However, there has been limited research effort to characterize the Ethiopian white lupin landraces. Fifteen polymorphic simple sequence repeat (SSR) m...

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Authors: Atnaf, M., Yao, N., Kyalo, M., Kifle, D., Dagne Wegary Gissa, Kassahun Tesfaye
Format: article
Status:Published version
Publication Date:2017
Country:México
Institution:Centro Internacional de Mejoramiento de Maíz y Trigo
Repository:Repositorio Institucional de Publicaciones Multimedia del CIMMYT
OAI Identifier:oai:repository.cimmyt.org:10883/19181
Online Access:http://hdl.handle.net/10883/19181
Access Level:Open access
Keyword:AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
LUPINUS ALBUS
MOLECULAR GENETICS
PLANT BREEDING
GERMPLASM CONSERVATION
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spelling Molecular genetic diversity and population structure of Ethiopian white lupin landraces: implications for breeding and conservationAtnaf, M.Yao, N.Kyalo, M.Kifle, D.Dagne Wegary GissaKassahun TesfayeAGRICULTURAL SCIENCES AND BIOTECHNOLOGYLUPINUS ALBUSMOLECULAR GENETICSPLANT BREEDINGGERMPLASM CONSERVATIONWhite lupin is one of the four economically important species of the Lupinus genus and is an important grain legume in the Ethiopian farming system. However, there has been limited research effort to characterize the Ethiopian white lupin landraces. Fifteen polymorphic simple sequence repeat (SSR) markers were used to assess the genetic diversity and population structure of 212 Ethiopian white lupin (Lupinus albus) landraces and two genotypes from different species (Lupinus angustifolius and Lupinus mutabilis) were used as out-group. The SSR markers revealed 108 different alleles, 98 of them from 212 landraces and 10 from out-group genotypes, with an average of 6.5 alleles per locus. The average gene diversity was 0.31. Twenty eight landraces harbored one or more private alleles from the total of 28 private alleles identified in the 212 white lupin accessions. Seventy-seven rare alleles with a frequency of less than 5% were identified and accounted for 78.6% of the total alleles detected. Analysis of molecular variance (AMOVA) showed that 92% of allelic diversity was attributed to individual accessions within populations while only 8% was distributed among populations. At 70% similarity level, the UPGMA dendrogram resulted in the formation of 13 clusters comprised of 2 to 136 landraces, with the out-group genotypes and five landraces remaining distinct and ungrouped. Population differentiation and genetic distance were relatively high between Gondar and Ethiopian white lupin populations collected by Australians. A model-based population structure analysis divided the white lupin landraces into two populations. All Ethiopian white lupin landrace populations, except most of the landraces collected by Australians (77%) and about 44% from Awi, were grouped together with significant admixtures. The study also suggested that 34 accessions, as core collections, were sufficient to retain 100% of SSR diversity. These accessions (core G-34) represent 16% of the whole 212 Ethiopian white lupin accessions and populations from West Gojam, Awi and Australian collections contributed more accessions to the core collection.Public Library of Science2018-01-24T17:26:22Z2018-01-24T17:26:22Z2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePDFapplication/pdfhttp://hdl.handle.net/10883/1918110.1371/journal.pone.01886961112PLoS ONEreponame:Repositorio Institucional de Publicaciones Multimedia del CIMMYTinstname:Centro Internacional de Mejoramiento de Maíz y Trigoinstacron:CIMMYTEnglishhttps://ndownloader.figshare.com/articles/5653651/versions/1ETHIOPIAEthiopiaSan Francisco, USACIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose.Open Accessinfo:eu-repo/semantics/openAccessoai:repository.cimmyt.org:10883/191812024-10-11T19:59:11Z
dc.title.none.fl_str_mv Molecular genetic diversity and population structure of Ethiopian white lupin landraces: implications for breeding and conservation
title Molecular genetic diversity and population structure of Ethiopian white lupin landraces: implications for breeding and conservation
spellingShingle Molecular genetic diversity and population structure of Ethiopian white lupin landraces: implications for breeding and conservation
Atnaf, M.
AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
LUPINUS ALBUS
MOLECULAR GENETICS
PLANT BREEDING
GERMPLASM CONSERVATION
title_short Molecular genetic diversity and population structure of Ethiopian white lupin landraces: implications for breeding and conservation
title_full Molecular genetic diversity and population structure of Ethiopian white lupin landraces: implications for breeding and conservation
title_fullStr Molecular genetic diversity and population structure of Ethiopian white lupin landraces: implications for breeding and conservation
title_full_unstemmed Molecular genetic diversity and population structure of Ethiopian white lupin landraces: implications for breeding and conservation
title_sort Molecular genetic diversity and population structure of Ethiopian white lupin landraces: implications for breeding and conservation
dc.creator.none.fl_str_mv Atnaf, M.
Yao, N.
Kyalo, M.
Kifle, D.
Dagne Wegary Gissa
Kassahun Tesfaye
author Atnaf, M.
author_facet Atnaf, M.
Yao, N.
Kyalo, M.
Kifle, D.
Dagne Wegary Gissa
Kassahun Tesfaye
author_role author
author2 Yao, N.
Kyalo, M.
Kifle, D.
Dagne Wegary Gissa
Kassahun Tesfaye
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
LUPINUS ALBUS
MOLECULAR GENETICS
PLANT BREEDING
GERMPLASM CONSERVATION
topic AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
LUPINUS ALBUS
MOLECULAR GENETICS
PLANT BREEDING
GERMPLASM CONSERVATION
description White lupin is one of the four economically important species of the Lupinus genus and is an important grain legume in the Ethiopian farming system. However, there has been limited research effort to characterize the Ethiopian white lupin landraces. Fifteen polymorphic simple sequence repeat (SSR) markers were used to assess the genetic diversity and population structure of 212 Ethiopian white lupin (Lupinus albus) landraces and two genotypes from different species (Lupinus angustifolius and Lupinus mutabilis) were used as out-group. The SSR markers revealed 108 different alleles, 98 of them from 212 landraces and 10 from out-group genotypes, with an average of 6.5 alleles per locus. The average gene diversity was 0.31. Twenty eight landraces harbored one or more private alleles from the total of 28 private alleles identified in the 212 white lupin accessions. Seventy-seven rare alleles with a frequency of less than 5% were identified and accounted for 78.6% of the total alleles detected. Analysis of molecular variance (AMOVA) showed that 92% of allelic diversity was attributed to individual accessions within populations while only 8% was distributed among populations. At 70% similarity level, the UPGMA dendrogram resulted in the formation of 13 clusters comprised of 2 to 136 landraces, with the out-group genotypes and five landraces remaining distinct and ungrouped. Population differentiation and genetic distance were relatively high between Gondar and Ethiopian white lupin populations collected by Australians. A model-based population structure analysis divided the white lupin landraces into two populations. All Ethiopian white lupin landrace populations, except most of the landraces collected by Australians (77%) and about 44% from Awi, were grouped together with significant admixtures. The study also suggested that 34 accessions, as core collections, were sufficient to retain 100% of SSR diversity. These accessions (core G-34) represent 16% of the whole 212 Ethiopian white lupin accessions and populations from West Gojam, Awi and Australian collections contributed more accessions to the core collection.
publishDate 2017
dc.date.none.fl_str_mv 2017
2018-01-24T17:26:22Z
2018-01-24T17:26:22Z
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10883/19181
10.1371/journal.pone.0188696
url http://hdl.handle.net/10883/19181
identifier_str_mv 10.1371/journal.pone.0188696
dc.language.none.fl_str_mv English
language_invalid_str_mv English
dc.relation.none.fl_str_mv https://ndownloader.figshare.com/articles/5653651/versions/1
dc.rights.none.fl_str_mv Open Access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Open Access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv PDF
application/pdf
dc.coverage.none.fl_str_mv ETHIOPIA
Ethiopia
San Francisco, USA
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv 11
12
PLoS ONE
reponame:Repositorio Institucional de Publicaciones Multimedia del CIMMYT
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instacron:CIMMYT
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