MOUSE-ERYTHROCYTE MICRONUCLEUS (Mus-EMN) ASSAY ON THE CLASTOGENICITY OF INDUSTRIAL WASTEWATER
The Mouse-Erythrocyte-Micronucleus (Mus-EMN) assay is a modified mouse-micronucleus test which conventionally scom the micronucleus frequencies in bone marrow polychromatic etythrocyíes (PCE). In the Mus-EMN assay, MN frequency is scored in mature normachromatic erythrocytes (NCE) in the circulating...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2012 |
| País: | México |
| Institución: | UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO |
| Repositorio: | Revista Internacional de Contaminación Ambiental |
| Idioma: | español |
| OAI Identifier: | oai:ojs.pkp.sfu.ca:article/30990 |
| Acceso en línea: | https://www.revistascca.unam.mx/rica/index.php/rica/article/view/30990 |
| Access Level: | acceso abierto |
| Palabra clave: | mouse erythrocytes micronucleus clastogenicity wastewater |
| Sumario: | The Mouse-Erythrocyte-Micronucleus (Mus-EMN) assay is a modified mouse-micronucleus test which conventionally scom the micronucleus frequencies in bone marrow polychromatic etythrocyíes (PCE). In the Mus-EMN assay, MN frequency is scored in mature normachromatic erythrocytes (NCE) in the circulatingblood obtained fmm the tail or the eye socket of the mouse. This simplifd in vivo Mus-EMN assay was used to monitor the chronic clastogenicity of industrial wastewater throughout one year in the cumt study. Twenty four young (2 month old) CDl white mice were divided into 3 groups of 8, with 4 males and 4 females in each group, and caged individually for this study. Two treated groups of animals were fed with industrial wastewater cdlected weekly fmm the down stm of the Arena Canal (wastewater dispoal system) from the Benito Juarez Industrial District of the Queretaro Ciíy of Mexico. In order to reduce the toxicity, wastewater samples were diluted with tapwater at 1:s ratio (wastewater: tapwater) for treated group 1 and a 1:2 ratio fortreated group 2. Animals of the control group drank the tapwater. Red blood samples were collected monthlyfrom the tail and blood smears were double stained with hernatoxylin and Giemsa, and about 10,000 mature red blood cells were scored from each of the 8 slides of the experimental groups to derive the means and standard emrs. Results of this year-mund study showed a significant increase in MN frequencies (5.08 - 9.80 MN11000 cells) in the treated groups during the months of Octuber through January of the following year, the dry season of this area of Mexico. The MN frequencies of the treated mice declined to the control level (1.29 - 3.20 MN/1000 cells) afier 6-7 months of continuous exposure. Results of this study indicate that the Mus-EMN assay is adequate for chronic clastogenicity tests of water pollutants with the maximum time limit around 6 months which is abwt 20% of the youthful life of the mouse. |
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